Development and Characterization of Magnetic SARS-CoV-2 Peptide-Imprinted Polymers

The development of new methods for the rapid, sensitive, and selective detection of SARS-CoV-2 is a key factor in overcoming the global pandemic that we have been facing for over a year. In this work, we focused on the preparation of magnetic molecularly imprinted polymers (MMIPs) based on the self-...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Beatriz Fresco-Cala, Soumya Rajpal, Tamara Rudolf, Benedikt Keitel, Rüdiger Groß, Jan Münch, Alex D. Batista, Boris Mizaikoff
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dopamine molecularly imprinted polymers magnetic imprinted particles peptide imprinting epitope imprinting surrogate imprinting Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/0101b1c968d94897a2528f1af421ea96
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0101b1c968d94897a2528f1af421ea96
record_format	dspace
spelling	oai:doaj.org-article:0101b1c968d94897a2528f1af421ea962021-11-25T18:31:25ZDevelopment and Characterization of Magnetic SARS-CoV-2 Peptide-Imprinted Polymers10.3390/nano111129852079-4991https://doaj.org/article/0101b1c968d94897a2528f1af421ea962021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2985https://doaj.org/toc/2079-4991The development of new methods for the rapid, sensitive, and selective detection of SARS-CoV-2 is a key factor in overcoming the global pandemic that we have been facing for over a year. In this work, we focused on the preparation of magnetic molecularly imprinted polymers (MMIPs) based on the self-polymerization of dopamine at the surface of magnetic nanoparticles (MNPs). Instead of using the whole SARS-CoV-2 virion as a template, a peptide of the viral spike protein, which is present at the viral surface, was innovatively used for the imprinting step. Thus, problems associated with the infectious nature of the virus along with its potential instability when used as a template and under the polymerization conditions were avoided. Dopamine was selected as a functional monomer following a rational computational screening approach that revealed not only a high binding energy of the dopamine–peptide complex but also multi-point interactions across the entire peptide template surface as opposed to other monomers with similar binding affinity. Moreover, variables affecting the imprinting efficiency including polymerization time and amount of peptide and dopamine were experimentally evaluated. Finally, the selectivity of the prepared MMIPs vs. other peptide sequences (i.e., from Zika virus) was evaluated, demonstrating that the developed MMIPs were only specific for the target SARS-CoV-2 peptide.Beatriz Fresco-CalaSoumya RajpalTamara RudolfBenedikt KeitelRüdiger GroßJan MünchAlex D. BatistaBoris MizaikoffMDPI AGarticledopaminemolecularly imprinted polymersmagnetic imprinted particlespeptide imprintingepitope imprintingsurrogate imprintingChemistryQD1-999ENNanomaterials, Vol 11, Iss 2985, p 2985 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dopamine molecularly imprinted polymers magnetic imprinted particles peptide imprinting epitope imprinting surrogate imprinting Chemistry QD1-999
spellingShingle	dopamine molecularly imprinted polymers magnetic imprinted particles peptide imprinting epitope imprinting surrogate imprinting Chemistry QD1-999 Beatriz Fresco-Cala Soumya Rajpal Tamara Rudolf Benedikt Keitel Rüdiger Groß Jan Münch Alex D. Batista Boris Mizaikoff Development and Characterization of Magnetic SARS-CoV-2 Peptide-Imprinted Polymers
description	The development of new methods for the rapid, sensitive, and selective detection of SARS-CoV-2 is a key factor in overcoming the global pandemic that we have been facing for over a year. In this work, we focused on the preparation of magnetic molecularly imprinted polymers (MMIPs) based on the self-polymerization of dopamine at the surface of magnetic nanoparticles (MNPs). Instead of using the whole SARS-CoV-2 virion as a template, a peptide of the viral spike protein, which is present at the viral surface, was innovatively used for the imprinting step. Thus, problems associated with the infectious nature of the virus along with its potential instability when used as a template and under the polymerization conditions were avoided. Dopamine was selected as a functional monomer following a rational computational screening approach that revealed not only a high binding energy of the dopamine–peptide complex but also multi-point interactions across the entire peptide template surface as opposed to other monomers with similar binding affinity. Moreover, variables affecting the imprinting efficiency including polymerization time and amount of peptide and dopamine were experimentally evaluated. Finally, the selectivity of the prepared MMIPs vs. other peptide sequences (i.e., from Zika virus) was evaluated, demonstrating that the developed MMIPs were only specific for the target SARS-CoV-2 peptide.
format	article
author	Beatriz Fresco-Cala Soumya Rajpal Tamara Rudolf Benedikt Keitel Rüdiger Groß Jan Münch Alex D. Batista Boris Mizaikoff
author_facet	Beatriz Fresco-Cala Soumya Rajpal Tamara Rudolf Benedikt Keitel Rüdiger Groß Jan Münch Alex D. Batista Boris Mizaikoff
author_sort	Beatriz Fresco-Cala
title	Development and Characterization of Magnetic SARS-CoV-2 Peptide-Imprinted Polymers
title_short	Development and Characterization of Magnetic SARS-CoV-2 Peptide-Imprinted Polymers
title_full	Development and Characterization of Magnetic SARS-CoV-2 Peptide-Imprinted Polymers
title_fullStr	Development and Characterization of Magnetic SARS-CoV-2 Peptide-Imprinted Polymers
title_full_unstemmed	Development and Characterization of Magnetic SARS-CoV-2 Peptide-Imprinted Polymers
title_sort	development and characterization of magnetic sars-cov-2 peptide-imprinted polymers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0101b1c968d94897a2528f1af421ea96
work_keys_str_mv	AT beatrizfrescocala developmentandcharacterizationofmagneticsarscov2peptideimprintedpolymers AT soumyarajpal developmentandcharacterizationofmagneticsarscov2peptideimprintedpolymers AT tamararudolf developmentandcharacterizationofmagneticsarscov2peptideimprintedpolymers AT benediktkeitel developmentandcharacterizationofmagneticsarscov2peptideimprintedpolymers AT rudigergroß developmentandcharacterizationofmagneticsarscov2peptideimprintedpolymers AT janmunch developmentandcharacterizationofmagneticsarscov2peptideimprintedpolymers AT alexdbatista developmentandcharacterizationofmagneticsarscov2peptideimprintedpolymers AT borismizaikoff developmentandcharacterizationofmagneticsarscov2peptideimprintedpolymers
_version_	1718410994629214208

Development and Characterization of Magnetic SARS-CoV-2 Peptide-Imprinted Polymers

Ejemplares similares