Development of a highly specific serodiagnostic ELISA for West Nile virus infection using subviral particles

Abstract West Nile virus (WNV), a member of the Japanese encephalitis virus (JEV) serocomplex group, causes lethal encephalitis in humans and horses. Because serodiagnosis of WNV and JEV is hampered by cross-reactivity, the development of a simple, secure, and WNV-specific serodiagnostic system is r...

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Autores principales:	Keisuke Maezono, Shintaro Kobayashi, Koshiro Tabata, Kentaro Yoshii, Hiroaki Kariwa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0102c8277a8b4e8e889b126c4889e409
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spelling	oai:doaj.org-article:0102c8277a8b4e8e889b126c4889e4092021-12-02T17:39:32ZDevelopment of a highly specific serodiagnostic ELISA for West Nile virus infection using subviral particles10.1038/s41598-021-88777-52045-2322https://doaj.org/article/0102c8277a8b4e8e889b126c4889e4092021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88777-5https://doaj.org/toc/2045-2322Abstract West Nile virus (WNV), a member of the Japanese encephalitis virus (JEV) serocomplex group, causes lethal encephalitis in humans and horses. Because serodiagnosis of WNV and JEV is hampered by cross-reactivity, the development of a simple, secure, and WNV-specific serodiagnostic system is required. The coexpression of prM protein and E protein leads to the secretion of subviral particles (SPs). Deletion of the C-terminal region of E protein is reported to affect the production of SPs by some flaviviruses. However, the influence of such a deletion on the properties and antigenicity of WNV E protein is unclear. We analyzed the properties of full-length E protein and E proteins lacking the C-terminal region as novel serodiagnostics for WNV infection. Deletion of the C-terminal region of E protein suppressed the formation of SPs but did not affect the production of E protein. The sensitivity of an enzyme-linked immunosorbent assay (ELISA) using the full-length E protein was higher than that using the truncated E proteins. Furthermore, in the ELISA using full-length E protein, there was little cross-reactivity with anti-JEV antibodies, and the sensitivity was similar to that of the neutralization test.Keisuke MaezonoShintaro KobayashiKoshiro TabataKentaro YoshiiHiroaki KariwaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keisuke Maezono Shintaro Kobayashi Koshiro Tabata Kentaro Yoshii Hiroaki Kariwa Development of a highly specific serodiagnostic ELISA for West Nile virus infection using subviral particles
description	Abstract West Nile virus (WNV), a member of the Japanese encephalitis virus (JEV) serocomplex group, causes lethal encephalitis in humans and horses. Because serodiagnosis of WNV and JEV is hampered by cross-reactivity, the development of a simple, secure, and WNV-specific serodiagnostic system is required. The coexpression of prM protein and E protein leads to the secretion of subviral particles (SPs). Deletion of the C-terminal region of E protein is reported to affect the production of SPs by some flaviviruses. However, the influence of such a deletion on the properties and antigenicity of WNV E protein is unclear. We analyzed the properties of full-length E protein and E proteins lacking the C-terminal region as novel serodiagnostics for WNV infection. Deletion of the C-terminal region of E protein suppressed the formation of SPs but did not affect the production of E protein. The sensitivity of an enzyme-linked immunosorbent assay (ELISA) using the full-length E protein was higher than that using the truncated E proteins. Furthermore, in the ELISA using full-length E protein, there was little cross-reactivity with anti-JEV antibodies, and the sensitivity was similar to that of the neutralization test.
format	article
author	Keisuke Maezono Shintaro Kobayashi Koshiro Tabata Kentaro Yoshii Hiroaki Kariwa
author_facet	Keisuke Maezono Shintaro Kobayashi Koshiro Tabata Kentaro Yoshii Hiroaki Kariwa
author_sort	Keisuke Maezono
title	Development of a highly specific serodiagnostic ELISA for West Nile virus infection using subviral particles
title_short	Development of a highly specific serodiagnostic ELISA for West Nile virus infection using subviral particles
title_full	Development of a highly specific serodiagnostic ELISA for West Nile virus infection using subviral particles
title_fullStr	Development of a highly specific serodiagnostic ELISA for West Nile virus infection using subviral particles
title_full_unstemmed	Development of a highly specific serodiagnostic ELISA for West Nile virus infection using subviral particles
title_sort	development of a highly specific serodiagnostic elisa for west nile virus infection using subviral particles
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0102c8277a8b4e8e889b126c4889e409
work_keys_str_mv	AT keisukemaezono developmentofahighlyspecificserodiagnosticelisaforwestnilevirusinfectionusingsubviralparticles AT shintarokobayashi developmentofahighlyspecificserodiagnosticelisaforwestnilevirusinfectionusingsubviralparticles AT koshirotabata developmentofahighlyspecificserodiagnosticelisaforwestnilevirusinfectionusingsubviralparticles AT kentaroyoshii developmentofahighlyspecificserodiagnosticelisaforwestnilevirusinfectionusingsubviralparticles AT hiroakikariwa developmentofahighlyspecificserodiagnosticelisaforwestnilevirusinfectionusingsubviralparticles
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Development of a highly specific serodiagnostic ELISA for West Nile virus infection using subviral particles

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