Calcium-siRNA Nanocomplexes Optimized by Bovine Serum Albumin Coating Can Achieve Convenient and Efficient siRNA Delivery for Periodontitis Therapy

Yang Wang,1 Wen Song,2 Yi Cui,3 Yang Zhang,1 Shenglin Mei,4 Qintao Wang1 1State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, Air Force Medical University, Xi’an, People’s Republic of China; 2State Key Laboratory of Military Stomatol...

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Autores principales:	Wang Y, Song W, Cui Y, Zhang Y, Mei S, Wang Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	bovine serum albumin calcium ions osteogenic differentiation small interfering rna Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/010456e7f9974ef28f31c63b1750ed35
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spelling	oai:doaj.org-article:010456e7f9974ef28f31c63b1750ed352021-12-02T14:58:42ZCalcium-siRNA Nanocomplexes Optimized by Bovine Serum Albumin Coating Can Achieve Convenient and Efficient siRNA Delivery for Periodontitis Therapy1178-2013https://doaj.org/article/010456e7f9974ef28f31c63b1750ed352020-11-01T00:00:00Zhttps://www.dovepress.com/calcium-sirna-nanocomplexes-optimized-by-bovine-serum-albumin-coating--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yang Wang,1 Wen Song,2 Yi Cui,3 Yang Zhang,1 Shenglin Mei,4 Qintao Wang1 1State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, Air Force Medical University, Xi’an, People’s Republic of China; 2State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, Air Force Medical University, Xi’an, People’s Republic of China; 3Equipment Department, Xijing Hospital, Air Force Medical University, Xi’an, People’s Republic of China; 4Department of Prosthodontics, College of Stomatology, Xi’an Jiaotong University, Xi’an, People’s Republic of ChinaCorrespondence: Qintao WangDepartment of Periodontology, School of Stomatology, Air Force Medical University, No. 145 West Changle Road, Xi’an 710032, People’s Republic of ChinaTel +86 2984 776 096Fax +0086 2983 223 047Email yznmbk@fmmu.edu.cnPurpose: Reducing toxicity, immunogenicity, and costs of small interfering RNAs (siRNA) carrier materials are key goals for RNA interference (RNAi) technology transition from bench to bed. Recently, calcium ions (Ca2+) have garnered attention as a novel, alternative material for delivering siRNA to cells. However, the tolerance for Ca2+ concentration varies in different cell types, which has limited its applications in vivo. Bovine serum albumin (BSA) can bind to Ca2+ through chelation. Moreover, BSA is a favorable coating material for nanoparticles owing to its excellent biocompatibility. Therefore, we hypothesized that coating Ca2+-siRNA with BSA helps buffer Ca2+ toxicity in vivo.Methods: BSA-Ca2+-siRNA nanoparticles were prepared, and the size, shape, encapsulation, and release efficiency were characterized using atomic force microscopy, scanning electronic microcopy, and gel electrophoresis. Binding nanoparticles were evaluated using attenuated total reflection-Fourier-transform infrared spectroscopy. The cellular uptake, intracellular release, cytotoxicity, and gene knockdown of nanoparticles were evaluated in periodontal ligament stem cells (PDLSCs) using laser-scanning confocal microscope, flow cytometry, and real-time quantitative polymerase chain reaction.Results: BSA and Ca2+-siRNA could form a stable nano-scale complex (&sim; 140 nm in diameter). The nanocomplexes could maintain siRNA release for more than 1 week in neutral phosphate-buffered saline (PBS) and could induce accelerated degradation in acidic PBS (pH 5.0). The nanoparticles were taken up by the cells, primarily through macropinocytosis, and were then released intracellularly through the acidification of endosomes/lysosomes. Importantly, the BSA-Ca2+ carrier had high transfection efficiency and biocompatibility both in vitro and in vivo. To demonstrate the therapeutic potential of our BSA coating-optimized Ca2+-siRNA technology, we showed that BSA-Ca2+-siWWP1 complexes strongly enhanced the osteogenic differentiation of inflammatory PDLSCs.Conclusion: BSA-Ca2+ could potentially be used for siRNA delivery, which is not only highly efficient and cost-effective but also biocompatible to host tissues owing to the BSA coating.Keywords: bovine serum albumin, calcium ions, osteogenic differentiation, small interfering RNAWang YSong WCui YZhang YMei SWang QDove Medical Pressarticlebovine serum albumincalcium ionsosteogenic differentiationsmall interfering rnaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 9241-9253 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bovine serum albumin calcium ions osteogenic differentiation small interfering rna Medicine (General) R5-920
spellingShingle	bovine serum albumin calcium ions osteogenic differentiation small interfering rna Medicine (General) R5-920 Wang Y Song W Cui Y Zhang Y Mei S Wang Q Calcium-siRNA Nanocomplexes Optimized by Bovine Serum Albumin Coating Can Achieve Convenient and Efficient siRNA Delivery for Periodontitis Therapy
description	Yang Wang,1 Wen Song,2 Yi Cui,3 Yang Zhang,1 Shenglin Mei,4 Qintao Wang1 1State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, Air Force Medical University, Xi’an, People’s Republic of China; 2State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, Air Force Medical University, Xi’an, People’s Republic of China; 3Equipment Department, Xijing Hospital, Air Force Medical University, Xi’an, People’s Republic of China; 4Department of Prosthodontics, College of Stomatology, Xi’an Jiaotong University, Xi’an, People’s Republic of ChinaCorrespondence: Qintao WangDepartment of Periodontology, School of Stomatology, Air Force Medical University, No. 145 West Changle Road, Xi’an 710032, People’s Republic of ChinaTel +86 2984 776 096Fax +0086 2983 223 047Email yznmbk@fmmu.edu.cnPurpose: Reducing toxicity, immunogenicity, and costs of small interfering RNAs (siRNA) carrier materials are key goals for RNA interference (RNAi) technology transition from bench to bed. Recently, calcium ions (Ca2+) have garnered attention as a novel, alternative material for delivering siRNA to cells. However, the tolerance for Ca2+ concentration varies in different cell types, which has limited its applications in vivo. Bovine serum albumin (BSA) can bind to Ca2+ through chelation. Moreover, BSA is a favorable coating material for nanoparticles owing to its excellent biocompatibility. Therefore, we hypothesized that coating Ca2+-siRNA with BSA helps buffer Ca2+ toxicity in vivo.Methods: BSA-Ca2+-siRNA nanoparticles were prepared, and the size, shape, encapsulation, and release efficiency were characterized using atomic force microscopy, scanning electronic microcopy, and gel electrophoresis. Binding nanoparticles were evaluated using attenuated total reflection-Fourier-transform infrared spectroscopy. The cellular uptake, intracellular release, cytotoxicity, and gene knockdown of nanoparticles were evaluated in periodontal ligament stem cells (PDLSCs) using laser-scanning confocal microscope, flow cytometry, and real-time quantitative polymerase chain reaction.Results: BSA and Ca2+-siRNA could form a stable nano-scale complex (&sim; 140 nm in diameter). The nanocomplexes could maintain siRNA release for more than 1 week in neutral phosphate-buffered saline (PBS) and could induce accelerated degradation in acidic PBS (pH 5.0). The nanoparticles were taken up by the cells, primarily through macropinocytosis, and were then released intracellularly through the acidification of endosomes/lysosomes. Importantly, the BSA-Ca2+ carrier had high transfection efficiency and biocompatibility both in vitro and in vivo. To demonstrate the therapeutic potential of our BSA coating-optimized Ca2+-siRNA technology, we showed that BSA-Ca2+-siWWP1 complexes strongly enhanced the osteogenic differentiation of inflammatory PDLSCs.Conclusion: BSA-Ca2+ could potentially be used for siRNA delivery, which is not only highly efficient and cost-effective but also biocompatible to host tissues owing to the BSA coating.Keywords: bovine serum albumin, calcium ions, osteogenic differentiation, small interfering RNA
format	article
author	Wang Y Song W Cui Y Zhang Y Mei S Wang Q
author_facet	Wang Y Song W Cui Y Zhang Y Mei S Wang Q
author_sort	Wang Y
title	Calcium-siRNA Nanocomplexes Optimized by Bovine Serum Albumin Coating Can Achieve Convenient and Efficient siRNA Delivery for Periodontitis Therapy
title_short	Calcium-siRNA Nanocomplexes Optimized by Bovine Serum Albumin Coating Can Achieve Convenient and Efficient siRNA Delivery for Periodontitis Therapy
title_full	Calcium-siRNA Nanocomplexes Optimized by Bovine Serum Albumin Coating Can Achieve Convenient and Efficient siRNA Delivery for Periodontitis Therapy
title_fullStr	Calcium-siRNA Nanocomplexes Optimized by Bovine Serum Albumin Coating Can Achieve Convenient and Efficient siRNA Delivery for Periodontitis Therapy
title_full_unstemmed	Calcium-siRNA Nanocomplexes Optimized by Bovine Serum Albumin Coating Can Achieve Convenient and Efficient siRNA Delivery for Periodontitis Therapy
title_sort	calcium-sirna nanocomplexes optimized by bovine serum albumin coating can achieve convenient and efficient sirna delivery for periodontitis therapy
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/010456e7f9974ef28f31c63b1750ed35
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Calcium-siRNA Nanocomplexes Optimized by Bovine Serum Albumin Coating Can Achieve Convenient and Efficient siRNA Delivery for Periodontitis Therapy

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