Liposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch

Chia-Hung Chen1,*, Yu-Ling Lin1,*, Yen-Ku Liu1, Pei-Juin He2, Ching-Min Lin1, Yi-Han Chiu2, Chang-Jer Wu3, Tian-Lu Cheng4, Shih-Jen Liu5,6,**, Kuang-Wen Liao1,2,**1Institute of Molecular Medicine and Bioengineering, 2Department of Biological Science and Technology, National Chiao Tung University, Hs...

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Autores principales:	Chen CH, Lin YL, Liu YK, He PJ, Lin CM, Chiu YH, Wu CJ, Cheng TL, Liu SJ, Liao KW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/01084217d3e048c697c907d73a147de9
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spelling	oai:doaj.org-article:01084217d3e048c697c907d73a147de92021-12-02T07:45:07ZLiposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch1176-91141178-2013https://doaj.org/article/01084217d3e048c697c907d73a147de92012-02-01T00:00:00Zhttp://www.dovepress.com/liposome-based-polymer-complex-as-a-novel-adjuvant-enhancement-of-spec-a9208https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Chia-Hung Chen1,, Yu-Ling Lin1,, Yen-Ku Liu1, Pei-Juin He2, Ching-Min Lin1, Yi-Han Chiu2, Chang-Jer Wu3, Tian-Lu Cheng4, Shih-Jen Liu5,6,, Kuang-Wen Liao1,2,1Institute of Molecular Medicine and Bioengineering, 2Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 3Department of Food Science, National Taiwan Ocean University, Keelung, 4Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, 5National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, 6Graduate Institute of Immunology, China Medical University, Taichung, Taiwan, , Chia-Hung Chen and Yu-Ling Lin contributed equally to this work*Kuang-Wen Liao and Shih-Jen Liu contributed equally to this workAbstract: The aim of vaccination is to induce appropriate immunity against pathogens. Antibody-mediated immunity is critical for protection against many virus diseases, although it is becoming more evident that coordinated, multifunctional immune responses lead to the most effective defense. Specific antibody (Ab) isotypes are more efficient at protecting against pathogen invasion in different locations in the body. For example, compared to other Ab isotypes, immunoglobulin (Ig) A provides more protection at mucosal areas. In this study, we developed a cationic lipopolymer (liposome-polyethylene glycol-polyethyleneimine complex [LPPC]) adjuvant that strongly adsorbs antigens or immunomodulators onto its surface to enhance or switch immune responses. The results demonstrate that LPPC enhances uptake ability, surface marker expression, proinflammatory cytokine release, and antigen presentation in mouse phagocytes. In contrast to Freund&#39;s adjuvant, LPPC preferentially activates Th1-immunity against antigens in vivo. With lipopolysaccharides or CpG oligodeoxynucleotides, LPPC dramatically enhances the IgA or IgG2A proportion of total Ig, even in hosts that have developed Th2 immunities and high IgG1 serum titers. Taken together, the results demonstrate that the LPPC adjuvant not only increases the immunogenicity of antigens but also modulates host immunity to produce an appropriate Ab isotype by combining with immunomodulators.Keywords: liposome-PEG-PEI complex, adjuvant, class switch, immunomodulator, vaccineChen CHLin YLLiu YKHe PJLin CMChiu YHWu CJCheng TLLiu SJLiao KWDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 607-621 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chen CH Lin YL Liu YK He PJ Lin CM Chiu YH Wu CJ Cheng TL Liu SJ Liao KW Liposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch
description	Chia-Hung Chen1,, Yu-Ling Lin1,, Yen-Ku Liu1, Pei-Juin He2, Ching-Min Lin1, Yi-Han Chiu2, Chang-Jer Wu3, Tian-Lu Cheng4, Shih-Jen Liu5,6,, Kuang-Wen Liao1,2,1Institute of Molecular Medicine and Bioengineering, 2Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 3Department of Food Science, National Taiwan Ocean University, Keelung, 4Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, 5National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, 6Graduate Institute of Immunology, China Medical University, Taichung, Taiwan, , Chia-Hung Chen and Yu-Ling Lin contributed equally to this work*Kuang-Wen Liao and Shih-Jen Liu contributed equally to this workAbstract: The aim of vaccination is to induce appropriate immunity against pathogens. Antibody-mediated immunity is critical for protection against many virus diseases, although it is becoming more evident that coordinated, multifunctional immune responses lead to the most effective defense. Specific antibody (Ab) isotypes are more efficient at protecting against pathogen invasion in different locations in the body. For example, compared to other Ab isotypes, immunoglobulin (Ig) A provides more protection at mucosal areas. In this study, we developed a cationic lipopolymer (liposome-polyethylene glycol-polyethyleneimine complex [LPPC]) adjuvant that strongly adsorbs antigens or immunomodulators onto its surface to enhance or switch immune responses. The results demonstrate that LPPC enhances uptake ability, surface marker expression, proinflammatory cytokine release, and antigen presentation in mouse phagocytes. In contrast to Freund&#39;s adjuvant, LPPC preferentially activates Th1-immunity against antigens in vivo. With lipopolysaccharides or CpG oligodeoxynucleotides, LPPC dramatically enhances the IgA or IgG2A proportion of total Ig, even in hosts that have developed Th2 immunities and high IgG1 serum titers. Taken together, the results demonstrate that the LPPC adjuvant not only increases the immunogenicity of antigens but also modulates host immunity to produce an appropriate Ab isotype by combining with immunomodulators.Keywords: liposome-PEG-PEI complex, adjuvant, class switch, immunomodulator, vaccine
format	article
author	Chen CH Lin YL Liu YK He PJ Lin CM Chiu YH Wu CJ Cheng TL Liu SJ Liao KW
author_facet	Chen CH Lin YL Liu YK He PJ Lin CM Chiu YH Wu CJ Cheng TL Liu SJ Liao KW
author_sort	Chen CH
title	Liposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch
title_short	Liposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch
title_full	Liposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch
title_fullStr	Liposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch
title_full_unstemmed	Liposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch
title_sort	liposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/01084217d3e048c697c907d73a147de9
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Liposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch

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