Circulatory <i>Endothelin 1-</i>Regulating RNAs Panel: Promising Biomarkers for Non-Invasive NAFLD/NASH Diagnosis and Stratification: Clinical and Molecular Pilot Study

Nonalcoholic fatty liver disease (NAFLD) is one of the major seeds of liver cirrhosis and hepatocellular carcinoma. There is no convenient reliable non-invasive early diagnostic tool available for NAFLD/NASH diagnosis and stratification. Recently, the role of cytosolic sensor, stimulator of interfer...

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Autores principales:	Reda Albadawy, Sara H. A. Agwa, Eman Khairy, Maha Saad, Naglaa El Touchy, Mohamed Othman, Mohamed El Kassas, Marwa Matboli
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	steatohepatitis NAFLD NASH TNF RNA RNAs panel Genetics QH426-470
Acceso en línea:	https://doaj.org/article/0111f9e9921d4d4d8ccfa708c7dc86cf
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spelling	oai:doaj.org-article:0111f9e9921d4d4d8ccfa708c7dc86cf2021-11-25T17:42:19ZCirculatory <i>Endothelin 1-</i>Regulating RNAs Panel: Promising Biomarkers for Non-Invasive NAFLD/NASH Diagnosis and Stratification: Clinical and Molecular Pilot Study10.3390/genes121118132073-4425https://doaj.org/article/0111f9e9921d4d4d8ccfa708c7dc86cf2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1813https://doaj.org/toc/2073-4425Nonalcoholic fatty liver disease (NAFLD) is one of the major seeds of liver cirrhosis and hepatocellular carcinoma. There is no convenient reliable non-invasive early diagnostic tool available for NAFLD/NASH diagnosis and stratification. Recently, the role of cytosolic sensor, stimulator of interferon genes (STING) signaling pathway in pathogenesis of nonalcoholic steatohepatitis (NASH) has been evidenced in research. We have selected <i>EDN1/TNF/MAPK3/EP300/hsa-miR-6888-5p/lncRNA RABGAP1L-DT-206</i> RNA panel from bioinformatics microarrays databases related to STING pathway and NAFLD/NASH pathogenesis. We have used reverse-transcriptase real-time polymerase chain reaction to assess the expression of the serum RNAs panel in NAFLD/NASH without suspicion of advanced fibrosis, NAFLD/with NASH patients with suspicion of advanced fibrosis and controls. Additionally, we have assessed the diagnostic performance of the Ribonucleic acid (RNA) panel. We have detected upregulation of the <i>EDN1</i> regulating RNAs panel expression in NAFLD/NASH cases compared to healthy controls. We concluded that this circulatory RNA panel could enable us to discriminate NAFLD/NASH cases from controls, and also NAFLD/NASH cases (F1, F2) from advanced fibrosis stages (F3, F4).Reda AlbadawySara H. A. AgwaEman KhairyMaha SaadNaglaa El TouchyMohamed OthmanMohamed El KassasMarwa MatboliMDPI AGarticlesteatohepatitisNAFLDNASHTNFRNARNAs panelGeneticsQH426-470ENGenes, Vol 12, Iss 1813, p 1813 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	steatohepatitis NAFLD NASH TNF RNA RNAs panel Genetics QH426-470
spellingShingle	steatohepatitis NAFLD NASH TNF RNA RNAs panel Genetics QH426-470 Reda Albadawy Sara H. A. Agwa Eman Khairy Maha Saad Naglaa El Touchy Mohamed Othman Mohamed El Kassas Marwa Matboli Circulatory <i>Endothelin 1-</i>Regulating RNAs Panel: Promising Biomarkers for Non-Invasive NAFLD/NASH Diagnosis and Stratification: Clinical and Molecular Pilot Study
description	Nonalcoholic fatty liver disease (NAFLD) is one of the major seeds of liver cirrhosis and hepatocellular carcinoma. There is no convenient reliable non-invasive early diagnostic tool available for NAFLD/NASH diagnosis and stratification. Recently, the role of cytosolic sensor, stimulator of interferon genes (STING) signaling pathway in pathogenesis of nonalcoholic steatohepatitis (NASH) has been evidenced in research. We have selected <i>EDN1/TNF/MAPK3/EP300/hsa-miR-6888-5p/lncRNA RABGAP1L-DT-206</i> RNA panel from bioinformatics microarrays databases related to STING pathway and NAFLD/NASH pathogenesis. We have used reverse-transcriptase real-time polymerase chain reaction to assess the expression of the serum RNAs panel in NAFLD/NASH without suspicion of advanced fibrosis, NAFLD/with NASH patients with suspicion of advanced fibrosis and controls. Additionally, we have assessed the diagnostic performance of the Ribonucleic acid (RNA) panel. We have detected upregulation of the <i>EDN1</i> regulating RNAs panel expression in NAFLD/NASH cases compared to healthy controls. We concluded that this circulatory RNA panel could enable us to discriminate NAFLD/NASH cases from controls, and also NAFLD/NASH cases (F1, F2) from advanced fibrosis stages (F3, F4).
format	article
author	Reda Albadawy Sara H. A. Agwa Eman Khairy Maha Saad Naglaa El Touchy Mohamed Othman Mohamed El Kassas Marwa Matboli
author_facet	Reda Albadawy Sara H. A. Agwa Eman Khairy Maha Saad Naglaa El Touchy Mohamed Othman Mohamed El Kassas Marwa Matboli
author_sort	Reda Albadawy
title	Circulatory <i>Endothelin 1-</i>Regulating RNAs Panel: Promising Biomarkers for Non-Invasive NAFLD/NASH Diagnosis and Stratification: Clinical and Molecular Pilot Study
title_short	Circulatory <i>Endothelin 1-</i>Regulating RNAs Panel: Promising Biomarkers for Non-Invasive NAFLD/NASH Diagnosis and Stratification: Clinical and Molecular Pilot Study
title_full	Circulatory <i>Endothelin 1-</i>Regulating RNAs Panel: Promising Biomarkers for Non-Invasive NAFLD/NASH Diagnosis and Stratification: Clinical and Molecular Pilot Study
title_fullStr	Circulatory <i>Endothelin 1-</i>Regulating RNAs Panel: Promising Biomarkers for Non-Invasive NAFLD/NASH Diagnosis and Stratification: Clinical and Molecular Pilot Study
title_full_unstemmed	Circulatory <i>Endothelin 1-</i>Regulating RNAs Panel: Promising Biomarkers for Non-Invasive NAFLD/NASH Diagnosis and Stratification: Clinical and Molecular Pilot Study
title_sort	circulatory <i>endothelin 1-</i>regulating rnas panel: promising biomarkers for non-invasive nafld/nash diagnosis and stratification: clinical and molecular pilot study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0111f9e9921d4d4d8ccfa708c7dc86cf
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Circulatory <i>Endothelin 1-</i>Regulating RNAs Panel: Promising Biomarkers for Non-Invasive NAFLD/NASH Diagnosis and Stratification: Clinical and Molecular Pilot Study

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