Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, <i>Anguilla japonica,</i> According to Sex-Related Gene Expressions

The gonochoristic feature with environmental sex determination that occurs during the yellow stage in the eel provides an interesting model to investigate the mechanisms of gonadal development. We previously studied various sex-related genes during gonadal sex differentiation in Japanese eels. In th...

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Autores principales:	Chien-Ju Lin, Shan-Ru Jeng, Zhen-Yuan Lei, Wen-Shiun Yueh, Sylvie Dufour, Guan-Chung Wu, Ching-Fong Chang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>amh</i> <i>gsdf</i> gonadal development sex differentiation fish eel Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/01156457d3564a019e7f699ac683429d
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spelling	oai:doaj.org-article:01156457d3564a019e7f699ac683429d2021-11-25T17:10:21ZInvolvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, <i>Anguilla japonica,</i> According to Sex-Related Gene Expressions10.3390/cells101130072073-4409https://doaj.org/article/01156457d3564a019e7f699ac683429d2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3007https://doaj.org/toc/2073-4409The gonochoristic feature with environmental sex determination that occurs during the yellow stage in the eel provides an interesting model to investigate the mechanisms of gonadal development. We previously studied various sex-related genes during gonadal sex differentiation in Japanese eels. In the present study, the members of transforming growth factor beta (TGF-β) superfamily were investigated. Transcript levels of anti-Müllerian hormone, its receptor, gonadal soma-derived factor (<i>amh</i>, <i>amhr2</i>, and <i>gsdf</i>, respectively) measured by real-time polymerase chain reaction (qPCR) showed a strong sexual dimorphism. Transcripts were dominantly expressed in the testis, and their levels significantly increased with testicular differentiation. In contrast, the expressions of <i>amh</i>, <i>amhr2,</i> and <i>gsdf</i> transcripts were low in the ovary of E2-feminized female eels. In situ hybridization detected <i>gsdf</i> (but not <i>amh</i>) transcript signals in undifferentiated gonads. <i>amh</i> and <i>gsdf</i> signals were localized to Sertoli cells and had increased significantly with testicular differentiation. Weak <i>gsdf</i> and no <i>amh</i> signals were detected in early ovaries of E2-feminized female eels. Transcript levels of <i>amh</i> and <i>gsdf</i> (not <i>amhr2</i>) decreased during human chorionic gonadotropin (HCG)-induced spermatogenesis in males. This study suggests that <i>amh</i>, <i>amhr2,</i> and especially <i>gsdf</i> might be involved in the gene pathway regulating testicular differentiation of Japanese eels.Chien-Ju LinShan-Ru JengZhen-Yuan LeiWen-Shiun YuehSylvie DufourGuan-Chung WuChing-Fong ChangMDPI AGarticle<i>amh</i><i>gsdf</i>gonadal developmentsex differentiationfisheelBiology (General)QH301-705.5ENCells, Vol 10, Iss 3007, p 3007 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>amh</i> <i>gsdf</i> gonadal development sex differentiation fish eel Biology (General) QH301-705.5
spellingShingle	<i>amh</i> <i>gsdf</i> gonadal development sex differentiation fish eel Biology (General) QH301-705.5 Chien-Ju Lin Shan-Ru Jeng Zhen-Yuan Lei Wen-Shiun Yueh Sylvie Dufour Guan-Chung Wu Ching-Fong Chang Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, <i>Anguilla japonica,</i> According to Sex-Related Gene Expressions
description	The gonochoristic feature with environmental sex determination that occurs during the yellow stage in the eel provides an interesting model to investigate the mechanisms of gonadal development. We previously studied various sex-related genes during gonadal sex differentiation in Japanese eels. In the present study, the members of transforming growth factor beta (TGF-β) superfamily were investigated. Transcript levels of anti-Müllerian hormone, its receptor, gonadal soma-derived factor (<i>amh</i>, <i>amhr2</i>, and <i>gsdf</i>, respectively) measured by real-time polymerase chain reaction (qPCR) showed a strong sexual dimorphism. Transcripts were dominantly expressed in the testis, and their levels significantly increased with testicular differentiation. In contrast, the expressions of <i>amh</i>, <i>amhr2,</i> and <i>gsdf</i> transcripts were low in the ovary of E2-feminized female eels. In situ hybridization detected <i>gsdf</i> (but not <i>amh</i>) transcript signals in undifferentiated gonads. <i>amh</i> and <i>gsdf</i> signals were localized to Sertoli cells and had increased significantly with testicular differentiation. Weak <i>gsdf</i> and no <i>amh</i> signals were detected in early ovaries of E2-feminized female eels. Transcript levels of <i>amh</i> and <i>gsdf</i> (not <i>amhr2</i>) decreased during human chorionic gonadotropin (HCG)-induced spermatogenesis in males. This study suggests that <i>amh</i>, <i>amhr2,</i> and especially <i>gsdf</i> might be involved in the gene pathway regulating testicular differentiation of Japanese eels.
format	article
author	Chien-Ju Lin Shan-Ru Jeng Zhen-Yuan Lei Wen-Shiun Yueh Sylvie Dufour Guan-Chung Wu Ching-Fong Chang
author_facet	Chien-Ju Lin Shan-Ru Jeng Zhen-Yuan Lei Wen-Shiun Yueh Sylvie Dufour Guan-Chung Wu Ching-Fong Chang
author_sort	Chien-Ju Lin
title	Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, <i>Anguilla japonica,</i> According to Sex-Related Gene Expressions
title_short	Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, <i>Anguilla japonica,</i> According to Sex-Related Gene Expressions
title_full	Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, <i>Anguilla japonica,</i> According to Sex-Related Gene Expressions
title_fullStr	Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, <i>Anguilla japonica,</i> According to Sex-Related Gene Expressions
title_full_unstemmed	Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, <i>Anguilla japonica,</i> According to Sex-Related Gene Expressions
title_sort	involvement of transforming growth factor beta family genes in gonadal differentiation in japanese eel, <i>anguilla japonica,</i> according to sex-related gene expressions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/01156457d3564a019e7f699ac683429d
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Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, <i>Anguilla japonica,</i> According to Sex-Related Gene Expressions

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