Nigella Sativa’s Protection Against 7,12 Dimethylbenz [A] Anthracene -Induced Colon Carcinogenesis in Rats

Colon cancer is the third most common cause of death from cancer worldwide. Recently, natural products have been widely used as an alternative therapy for colon cancer. Previous studies have reported that Nigella sativa has chemopreventive activity in vitro and in vivo.This study aimed to evaluate t...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Heny Ekowati, Firster Nugroho, Iskandar Sobri
Formato: article
Lenguaje:EN
ID
Publicado: Jenderal Soedirman University 2021
Materias:
Acceso en línea:https://doaj.org/article/0116fa20996843f596297470a2ff496c
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:0116fa20996843f596297470a2ff496c
record_format dspace
spelling oai:doaj.org-article:0116fa20996843f596297470a2ff496c2021-11-22T01:13:55ZNigella Sativa’s Protection Against 7,12 Dimethylbenz [A] Anthracene -Induced Colon Carcinogenesis in Rats1907-97612503-031010.20884/1.jm.2021.16.3.734https://doaj.org/article/0116fa20996843f596297470a2ff496c2021-11-01T00:00:00Zhttps://ojs.jmolekul.com/ojs/index.php/jm/article/view/734https://doaj.org/toc/1907-9761https://doaj.org/toc/2503-0310Colon cancer is the third most common cause of death from cancer worldwide. Recently, natural products have been widely used as an alternative therapy for colon cancer. Previous studies have reported that Nigella sativa has chemopreventive activity in vitro and in vivo.This study aimed to evaluate the effect of Nigella sativa seed (NSS) on rat-colon cell after initiation of 7,12-dimethylbenz [a] anthracene. Rats were divided into five groups, 12 rats in each group: Group I was given 7,12dimetilbenz [a] anthracene (DMBA) orally 20 mg/kgBW twice a week for five weeks, group V is the solvent control group was given corn oil. The other three groups were given DMBA + NSS, at the dosage of 250 mg/kgBW, 500 mg/kgBW and 750 mg/kgBW. NSS extract was dissolved in corn oil and administered daily per oral during the next two weeks before and during the initiation of DMBA. After 16 weeks, all rats were sacrificed. H&E staining showed that necrosis activity was lower in treated groups compared to DMBA group. AgNOR staining showed mAgNOR was significantly decrease following the increasing dose of NSS (250 mg/kgBW, 500 mg/kgBW and 750 mg/kgBW) were subsequently 1.62 ± 0.086, 1.60 ± 0.101 and 1.39 ± 0.049 (p<0.05). The results showed that NNS reduce the damage of colon cells and inhibit colon cell proliferation in DMBA induced rats.Heny EkowatiFirster NugrohoIskandar SobriJenderal Soedirman UniversityarticleChemistryQD1-999ENIDMolekul, Vol 16, Iss 3, Pp 260-266 (2021)
institution DOAJ
collection DOAJ
language EN
ID
topic Chemistry
QD1-999
spellingShingle Chemistry
QD1-999
Heny Ekowati
Firster Nugroho
Iskandar Sobri
Nigella Sativa’s Protection Against 7,12 Dimethylbenz [A] Anthracene -Induced Colon Carcinogenesis in Rats
description Colon cancer is the third most common cause of death from cancer worldwide. Recently, natural products have been widely used as an alternative therapy for colon cancer. Previous studies have reported that Nigella sativa has chemopreventive activity in vitro and in vivo.This study aimed to evaluate the effect of Nigella sativa seed (NSS) on rat-colon cell after initiation of 7,12-dimethylbenz [a] anthracene. Rats were divided into five groups, 12 rats in each group: Group I was given 7,12dimetilbenz [a] anthracene (DMBA) orally 20 mg/kgBW twice a week for five weeks, group V is the solvent control group was given corn oil. The other three groups were given DMBA + NSS, at the dosage of 250 mg/kgBW, 500 mg/kgBW and 750 mg/kgBW. NSS extract was dissolved in corn oil and administered daily per oral during the next two weeks before and during the initiation of DMBA. After 16 weeks, all rats were sacrificed. H&E staining showed that necrosis activity was lower in treated groups compared to DMBA group. AgNOR staining showed mAgNOR was significantly decrease following the increasing dose of NSS (250 mg/kgBW, 500 mg/kgBW and 750 mg/kgBW) were subsequently 1.62 ± 0.086, 1.60 ± 0.101 and 1.39 ± 0.049 (p<0.05). The results showed that NNS reduce the damage of colon cells and inhibit colon cell proliferation in DMBA induced rats.
format article
author Heny Ekowati
Firster Nugroho
Iskandar Sobri
author_facet Heny Ekowati
Firster Nugroho
Iskandar Sobri
author_sort Heny Ekowati
title Nigella Sativa’s Protection Against 7,12 Dimethylbenz [A] Anthracene -Induced Colon Carcinogenesis in Rats
title_short Nigella Sativa’s Protection Against 7,12 Dimethylbenz [A] Anthracene -Induced Colon Carcinogenesis in Rats
title_full Nigella Sativa’s Protection Against 7,12 Dimethylbenz [A] Anthracene -Induced Colon Carcinogenesis in Rats
title_fullStr Nigella Sativa’s Protection Against 7,12 Dimethylbenz [A] Anthracene -Induced Colon Carcinogenesis in Rats
title_full_unstemmed Nigella Sativa’s Protection Against 7,12 Dimethylbenz [A] Anthracene -Induced Colon Carcinogenesis in Rats
title_sort nigella sativa’s protection against 7,12 dimethylbenz [a] anthracene -induced colon carcinogenesis in rats
publisher Jenderal Soedirman University
publishDate 2021
url https://doaj.org/article/0116fa20996843f596297470a2ff496c
work_keys_str_mv AT henyekowati nigellasativasprotectionagainst712dimethylbenzaanthraceneinducedcoloncarcinogenesisinrats
AT firsternugroho nigellasativasprotectionagainst712dimethylbenzaanthraceneinducedcoloncarcinogenesisinrats
AT iskandarsobri nigellasativasprotectionagainst712dimethylbenzaanthraceneinducedcoloncarcinogenesisinrats
_version_ 1718418261475852288