Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.

The F region downstream of the mecI gene in the SCCmec element in hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) contains two bidirectionally overlapping open reading frames (ORFs), the fudoh ORF and the psm-mec ORF. The psm-mec ORF encodes a cytolysin, phenol-soluble modu...

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Autores principales:	Chikara Kaito, Yuki Saito, Gentaro Nagano, Mariko Ikuo, Yosuke Omae, Yuichi Hanada, Xiao Han, Kyoko Kuwahara-Arai, Tomomi Hishinuma, Tadashi Baba, Teruyo Ito, Keiichi Hiramatsu, Kazuhisa Sekimizu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/01181988587c4409935821e4ed22c88e
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spelling	oai:doaj.org-article:01181988587c4409935821e4ed22c88e2021-11-18T06:03:38ZTranscription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.1553-73661553-737410.1371/journal.ppat.1001267https://doaj.org/article/01181988587c4409935821e4ed22c88e2011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21304931/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The F region downstream of the mecI gene in the SCCmec element in hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) contains two bidirectionally overlapping open reading frames (ORFs), the fudoh ORF and the psm-mec ORF. The psm-mec ORF encodes a cytolysin, phenol-soluble modulin (PSM)-mec. Transformation of the F region into the Newman strain, which is a methicillin-sensitive S. aureus (MSSA) strain, or into the MW2 (USA400) and FRP3757 (USA300) strains, which are community-acquired MRSA (CA-MRSA) strains that lack the F region, attenuated their virulence in a mouse systemic infection model. Introducing the F region to these strains suppressed colony-spreading activity and PSMα production, and promoted biofilm formation. By producing mutations into the psm-mec ORF, we revealed that (i) both the transcription and translation products of the psm-mec ORF suppressed colony-spreading activity and promoted biofilm formation; and (ii) the transcription product of the psm-mec ORF, but not its translation product, decreased PSMα production. These findings suggest that both the psm-mec transcript, acting as a regulatory RNA, and the PSM-mec protein encoded by the gene on the mobile genetic element SCCmec regulate the virulence of Staphylococcus aureus.Chikara KaitoYuki SaitoGentaro NaganoMariko IkuoYosuke OmaeYuichi HanadaXiao HanKyoko Kuwahara-AraiTomomi HishinumaTadashi BabaTeruyo ItoKeiichi HiramatsuKazuhisa SekimizuPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 2, p e1001267 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Chikara Kaito Yuki Saito Gentaro Nagano Mariko Ikuo Yosuke Omae Yuichi Hanada Xiao Han Kyoko Kuwahara-Arai Tomomi Hishinuma Tadashi Baba Teruyo Ito Keiichi Hiramatsu Kazuhisa Sekimizu Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.
description	The F region downstream of the mecI gene in the SCCmec element in hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) contains two bidirectionally overlapping open reading frames (ORFs), the fudoh ORF and the psm-mec ORF. The psm-mec ORF encodes a cytolysin, phenol-soluble modulin (PSM)-mec. Transformation of the F region into the Newman strain, which is a methicillin-sensitive S. aureus (MSSA) strain, or into the MW2 (USA400) and FRP3757 (USA300) strains, which are community-acquired MRSA (CA-MRSA) strains that lack the F region, attenuated their virulence in a mouse systemic infection model. Introducing the F region to these strains suppressed colony-spreading activity and PSMα production, and promoted biofilm formation. By producing mutations into the psm-mec ORF, we revealed that (i) both the transcription and translation products of the psm-mec ORF suppressed colony-spreading activity and promoted biofilm formation; and (ii) the transcription product of the psm-mec ORF, but not its translation product, decreased PSMα production. These findings suggest that both the psm-mec transcript, acting as a regulatory RNA, and the PSM-mec protein encoded by the gene on the mobile genetic element SCCmec regulate the virulence of Staphylococcus aureus.
format	article
author	Chikara Kaito Yuki Saito Gentaro Nagano Mariko Ikuo Yosuke Omae Yuichi Hanada Xiao Han Kyoko Kuwahara-Arai Tomomi Hishinuma Tadashi Baba Teruyo Ito Keiichi Hiramatsu Kazuhisa Sekimizu
author_facet	Chikara Kaito Yuki Saito Gentaro Nagano Mariko Ikuo Yosuke Omae Yuichi Hanada Xiao Han Kyoko Kuwahara-Arai Tomomi Hishinuma Tadashi Baba Teruyo Ito Keiichi Hiramatsu Kazuhisa Sekimizu
author_sort	Chikara Kaito
title	Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.
title_short	Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.
title_full	Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.
title_fullStr	Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.
title_full_unstemmed	Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.
title_sort	transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element sccmec regulate staphylococcus aureus virulence.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/01181988587c4409935821e4ed22c88e
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Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence.

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