Protein-Based Systems for Translational Regulation of Synthetic mRNAs in Mammalian Cells

Synthetic mRNAs, which are produced by in vitro transcription, have been recently attracting attention because they can express any transgenes without the risk of insertional mutagenesis. Although current synthetic mRNA medicine is not designed for spatiotemporal or cell-selective regulation, many p...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Hideyuki Nakanishi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	synthetic biology messenger RNA RNA binding protein translation modified nucleoside Science Q
Acceso en línea:	https://doaj.org/article/012127803e3c449fb39f14f7c1190760
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:012127803e3c449fb39f14f7c1190760
record_format	dspace
spelling	oai:doaj.org-article:012127803e3c449fb39f14f7c11907602021-11-25T18:11:02ZProtein-Based Systems for Translational Regulation of Synthetic mRNAs in Mammalian Cells10.3390/life111111922075-1729https://doaj.org/article/012127803e3c449fb39f14f7c11907602021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1192https://doaj.org/toc/2075-1729Synthetic mRNAs, which are produced by in vitro transcription, have been recently attracting attention because they can express any transgenes without the risk of insertional mutagenesis. Although current synthetic mRNA medicine is not designed for spatiotemporal or cell-selective regulation, many preclinical studies have developed the systems for the translational regulation of synthetic mRNAs. Such translational regulation systems will cope with high efficacy and low adverse effects by producing the appropriate amount of therapeutic proteins, depending on the context. Protein-based regulation is one of the most promising approaches for the translational regulation of synthetic mRNAs. As synthetic mRNAs can encode not only output proteins but also regulator proteins, all components of protein-based regulation systems can be delivered as synthetic mRNAs. In addition, in the protein-based regulation systems, the output protein can be utilized as the input for the subsequent regulation to construct multi-layered gene circuits, which enable complex and sophisticated regulation. In this review, I introduce what types of proteins have been used for translational regulation, how to combine them, and how to design effective gene circuits.Hideyuki NakanishiMDPI AGarticlesynthetic biologymessenger RNARNA binding proteintranslationmodified nucleosideScienceQENLife, Vol 11, Iss 1192, p 1192 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	synthetic biology messenger RNA RNA binding protein translation modified nucleoside Science Q
spellingShingle	synthetic biology messenger RNA RNA binding protein translation modified nucleoside Science Q Hideyuki Nakanishi Protein-Based Systems for Translational Regulation of Synthetic mRNAs in Mammalian Cells
description	Synthetic mRNAs, which are produced by in vitro transcription, have been recently attracting attention because they can express any transgenes without the risk of insertional mutagenesis. Although current synthetic mRNA medicine is not designed for spatiotemporal or cell-selective regulation, many preclinical studies have developed the systems for the translational regulation of synthetic mRNAs. Such translational regulation systems will cope with high efficacy and low adverse effects by producing the appropriate amount of therapeutic proteins, depending on the context. Protein-based regulation is one of the most promising approaches for the translational regulation of synthetic mRNAs. As synthetic mRNAs can encode not only output proteins but also regulator proteins, all components of protein-based regulation systems can be delivered as synthetic mRNAs. In addition, in the protein-based regulation systems, the output protein can be utilized as the input for the subsequent regulation to construct multi-layered gene circuits, which enable complex and sophisticated regulation. In this review, I introduce what types of proteins have been used for translational regulation, how to combine them, and how to design effective gene circuits.
format	article
author	Hideyuki Nakanishi
author_facet	Hideyuki Nakanishi
author_sort	Hideyuki Nakanishi
title	Protein-Based Systems for Translational Regulation of Synthetic mRNAs in Mammalian Cells
title_short	Protein-Based Systems for Translational Regulation of Synthetic mRNAs in Mammalian Cells
title_full	Protein-Based Systems for Translational Regulation of Synthetic mRNAs in Mammalian Cells
title_fullStr	Protein-Based Systems for Translational Regulation of Synthetic mRNAs in Mammalian Cells
title_full_unstemmed	Protein-Based Systems for Translational Regulation of Synthetic mRNAs in Mammalian Cells
title_sort	protein-based systems for translational regulation of synthetic mrnas in mammalian cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/012127803e3c449fb39f14f7c1190760
work_keys_str_mv	AT hideyukinakanishi proteinbasedsystemsfortranslationalregulationofsyntheticmrnasinmammaliancells
_version_	1718411479253778432

Protein-Based Systems for Translational Regulation of Synthetic mRNAs in Mammalian Cells

Ejemplares similares