The long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells

Abstract In recent years, long non-coding RNA (lncRNA) research has identified essential roles of these transcripts in virtually all physiological cellular processes including tumorigenesis, but their functions and molecular mechanisms are poorly understood. In this study, we performed a high-throug...

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Autores principales:	Linda Nötzold, Lukas Frank, Minakshi Gandhi, Maria Polycarpou-Schwarz, Matthias Groß, Manuel Gunkel, Nina Beil, Holger Erfle, Nathalie Harder, Karl Rohr, Jakob Trendel, Jeroen Krijgsveld, Thomas Longerich, Peter Schirmacher, Michael Boutros, Sylvia Erhardt, Sven Diederichs
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0128d3ba3b3d4231913c4de6607365c4
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spelling	oai:doaj.org-article:0128d3ba3b3d4231913c4de6607365c42021-12-02T12:32:30ZThe long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells10.1038/s41598-017-02357-02045-2322https://doaj.org/article/0128d3ba3b3d4231913c4de6607365c42017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02357-0https://doaj.org/toc/2045-2322Abstract In recent years, long non-coding RNA (lncRNA) research has identified essential roles of these transcripts in virtually all physiological cellular processes including tumorigenesis, but their functions and molecular mechanisms are poorly understood. In this study, we performed a high-throughput siRNA screen targeting 638 lncRNAs deregulated in cancer entities to analyse their impact on cell division by using time-lapse microscopy. We identified 26 lncRNAs affecting cell morphology and cell cycle including LINC00152. This transcript was ubiquitously expressed in many human cell lines and its RNA levels were significantly upregulated in lung, liver and breast cancer tissues. A comprehensive sequence analysis of LINC00152 revealed a highly similar paralog annotated as MIR4435-2HG and several splice variants of both transcripts. The shortest and most abundant isoform preferentially localized to the cytoplasm. Cells depleted of LINC00152 arrested in prometaphase of mitosis and showed reduced cell viability. In RNA affinity purification (RAP) studies, LINC00152 interacted with a network of proteins that were associated with M phase of the cell cycle. In summary, we provide new insights into the properties and biological function of LINC00152 suggesting that this transcript is crucial for cell cycle progression through mitosis and thus, could act as a non-coding oncogene.Linda NötzoldLukas FrankMinakshi GandhiMaria Polycarpou-SchwarzMatthias GroßManuel GunkelNina BeilHolger ErfleNathalie HarderKarl RohrJakob TrendelJeroen KrijgsveldThomas LongerichPeter SchirmacherMichael BoutrosSylvia ErhardtSven DiederichsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Linda Nötzold Lukas Frank Minakshi Gandhi Maria Polycarpou-Schwarz Matthias Groß Manuel Gunkel Nina Beil Holger Erfle Nathalie Harder Karl Rohr Jakob Trendel Jeroen Krijgsveld Thomas Longerich Peter Schirmacher Michael Boutros Sylvia Erhardt Sven Diederichs The long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells
description	Abstract In recent years, long non-coding RNA (lncRNA) research has identified essential roles of these transcripts in virtually all physiological cellular processes including tumorigenesis, but their functions and molecular mechanisms are poorly understood. In this study, we performed a high-throughput siRNA screen targeting 638 lncRNAs deregulated in cancer entities to analyse their impact on cell division by using time-lapse microscopy. We identified 26 lncRNAs affecting cell morphology and cell cycle including LINC00152. This transcript was ubiquitously expressed in many human cell lines and its RNA levels were significantly upregulated in lung, liver and breast cancer tissues. A comprehensive sequence analysis of LINC00152 revealed a highly similar paralog annotated as MIR4435-2HG and several splice variants of both transcripts. The shortest and most abundant isoform preferentially localized to the cytoplasm. Cells depleted of LINC00152 arrested in prometaphase of mitosis and showed reduced cell viability. In RNA affinity purification (RAP) studies, LINC00152 interacted with a network of proteins that were associated with M phase of the cell cycle. In summary, we provide new insights into the properties and biological function of LINC00152 suggesting that this transcript is crucial for cell cycle progression through mitosis and thus, could act as a non-coding oncogene.
format	article
author	Linda Nötzold Lukas Frank Minakshi Gandhi Maria Polycarpou-Schwarz Matthias Groß Manuel Gunkel Nina Beil Holger Erfle Nathalie Harder Karl Rohr Jakob Trendel Jeroen Krijgsveld Thomas Longerich Peter Schirmacher Michael Boutros Sylvia Erhardt Sven Diederichs
author_facet	Linda Nötzold Lukas Frank Minakshi Gandhi Maria Polycarpou-Schwarz Matthias Groß Manuel Gunkel Nina Beil Holger Erfle Nathalie Harder Karl Rohr Jakob Trendel Jeroen Krijgsveld Thomas Longerich Peter Schirmacher Michael Boutros Sylvia Erhardt Sven Diederichs
author_sort	Linda Nötzold
title	The long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells
title_short	The long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells
title_full	The long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells
title_fullStr	The long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells
title_full_unstemmed	The long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells
title_sort	long non-coding rna linc00152 is essential for cell cycle progression through mitosis in hela cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0128d3ba3b3d4231913c4de6607365c4
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The long non-coding RNA LINC00152 is essential for cell cycle progression through mitosis in HeLa cells

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