Histones H3 and H4 require their relevant amino-tails for efficient nuclear import and replication-coupled chromatin assembly in vivo

Abstract Concomitant chromatin assembly and DNA duplication is essential for cell survival and genome integrity, and requires newly synthesized histones. Although the N-terminal domains of newly synthesized H3 and H4 present critical functions, their requirement for replication-coupled chromatin ass...

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Autores principales:	Aïda Ejlassi, Vanessa Menil-Philippot, Angélique Galvani, Christophe Thiriet
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/012a9b5448084b3aa74c975977cdf33b
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spelling	oai:doaj.org-article:012a9b5448084b3aa74c975977cdf33b2021-12-02T12:32:18ZHistones H3 and H4 require their relevant amino-tails for efficient nuclear import and replication-coupled chromatin assembly in vivo10.1038/s41598-017-03218-62045-2322https://doaj.org/article/012a9b5448084b3aa74c975977cdf33b2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03218-6https://doaj.org/toc/2045-2322Abstract Concomitant chromatin assembly and DNA duplication is essential for cell survival and genome integrity, and requires newly synthesized histones. Although the N-terminal domains of newly synthesized H3 and H4 present critical functions, their requirement for replication-coupled chromatin assembly is controversial. Using the unique capability of the spontaneous internalization of exogenous proteins in Physarum, we showed that H3 and H4 N-tails present critical functions in nuclear import during the S-phase, but are dispensable for assembly into nucleosomes. However, our data revealed that chromatin assembly in the S-phase of complexes presenting ectopic N-terminal domains occurs by a replication-independent mechanism. We found that replication-dependent chromatin assembly requires an H3/H4 complex with the relevant N-tail domains, suggesting a concomitant recognition of the two histone domains by histone chaperones.Aïda EjlassiVanessa Menil-PhilippotAngélique GalvaniChristophe ThirietNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aïda Ejlassi Vanessa Menil-Philippot Angélique Galvani Christophe Thiriet Histones H3 and H4 require their relevant amino-tails for efficient nuclear import and replication-coupled chromatin assembly in vivo
description	Abstract Concomitant chromatin assembly and DNA duplication is essential for cell survival and genome integrity, and requires newly synthesized histones. Although the N-terminal domains of newly synthesized H3 and H4 present critical functions, their requirement for replication-coupled chromatin assembly is controversial. Using the unique capability of the spontaneous internalization of exogenous proteins in Physarum, we showed that H3 and H4 N-tails present critical functions in nuclear import during the S-phase, but are dispensable for assembly into nucleosomes. However, our data revealed that chromatin assembly in the S-phase of complexes presenting ectopic N-terminal domains occurs by a replication-independent mechanism. We found that replication-dependent chromatin assembly requires an H3/H4 complex with the relevant N-tail domains, suggesting a concomitant recognition of the two histone domains by histone chaperones.
format	article
author	Aïda Ejlassi Vanessa Menil-Philippot Angélique Galvani Christophe Thiriet
author_facet	Aïda Ejlassi Vanessa Menil-Philippot Angélique Galvani Christophe Thiriet
author_sort	Aïda Ejlassi
title	Histones H3 and H4 require their relevant amino-tails for efficient nuclear import and replication-coupled chromatin assembly in vivo
title_short	Histones H3 and H4 require their relevant amino-tails for efficient nuclear import and replication-coupled chromatin assembly in vivo
title_full	Histones H3 and H4 require their relevant amino-tails for efficient nuclear import and replication-coupled chromatin assembly in vivo
title_fullStr	Histones H3 and H4 require their relevant amino-tails for efficient nuclear import and replication-coupled chromatin assembly in vivo
title_full_unstemmed	Histones H3 and H4 require their relevant amino-tails for efficient nuclear import and replication-coupled chromatin assembly in vivo
title_sort	histones h3 and h4 require their relevant amino-tails for efficient nuclear import and replication-coupled chromatin assembly in vivo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/012a9b5448084b3aa74c975977cdf33b
work_keys_str_mv	AT aidaejlassi histonesh3andh4requiretheirrelevantaminotailsforefficientnuclearimportandreplicationcoupledchromatinassemblyinvivo AT vanessamenilphilippot histonesh3andh4requiretheirrelevantaminotailsforefficientnuclearimportandreplicationcoupledchromatinassemblyinvivo AT angeliquegalvani histonesh3andh4requiretheirrelevantaminotailsforefficientnuclearimportandreplicationcoupledchromatinassemblyinvivo AT christophethiriet histonesh3andh4requiretheirrelevantaminotailsforefficientnuclearimportandreplicationcoupledchromatinassemblyinvivo
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Histones H3 and H4 require their relevant amino-tails for efficient nuclear import and replication-coupled chromatin assembly in vivo

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