Calcium dependence of eugenol tolerance and toxicity in Saccharomyces cerevisiae.

Eugenol is a plant-derived phenolic compound which has recognised therapeutical potential as an antifungal agent. However little is known of either its fungicidal activity or the mechanisms employed by fungi to tolerate eugenol toxicity. A better exploitation of eugenol as a therapeutic agent will t...

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Autores principales:	Stephen K Roberts, Martin McAinsh, Hanna Cantopher, Sean Sandison
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/012cd1cf88384a8fa8116bd1d389ce72
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spelling	oai:doaj.org-article:012cd1cf88384a8fa8116bd1d389ce722021-11-25T06:07:52ZCalcium dependence of eugenol tolerance and toxicity in Saccharomyces cerevisiae.1932-620310.1371/journal.pone.0102712https://doaj.org/article/012cd1cf88384a8fa8116bd1d389ce722014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25036027/?tool=EBIhttps://doaj.org/toc/1932-6203Eugenol is a plant-derived phenolic compound which has recognised therapeutical potential as an antifungal agent. However little is known of either its fungicidal activity or the mechanisms employed by fungi to tolerate eugenol toxicity. A better exploitation of eugenol as a therapeutic agent will therefore depend on addressing this knowledge gap. Eugenol initiates increases in cytosolic Ca2+ in Saccharomyces cerevisiae which is partly dependent on the plasma membrane calcium channel, Cch1p. However, it is unclear whether a toxic cytosolic Ca2+elevation mediates the fungicidal activity of eugenol. In the present study, no significant difference in yeast survival was observed following transient eugenol treatment in the presence or absence of extracellular Ca2+. Furthermore, using yeast expressing apoaequorin to report cytosolic Ca2+ and a range of eugenol derivatives, antifungal activity did not appear to be coupled to Ca2+ influx or cytosolic Ca2+ elevation. Taken together, these results suggest that eugenol toxicity is not dependent on a toxic influx of Ca2+. In contrast, careful control of extracellular Ca2+ (using EGTA or BAPTA) revealed that tolerance of yeast to eugenol depended on Ca2+ influx via Cch1p. These findings expose significant differences between the antifungal activity of eugenol and that of azoles, amiodarone and carvacrol. This study highlights the potential to use eugenol in combination with other antifungal agents that exhibit differing modes of action as antifungal agents to combat drug resistant infections.Stephen K RobertsMartin McAinshHanna CantopherSean SandisonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e102712 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stephen K Roberts Martin McAinsh Hanna Cantopher Sean Sandison Calcium dependence of eugenol tolerance and toxicity in Saccharomyces cerevisiae.
description	Eugenol is a plant-derived phenolic compound which has recognised therapeutical potential as an antifungal agent. However little is known of either its fungicidal activity or the mechanisms employed by fungi to tolerate eugenol toxicity. A better exploitation of eugenol as a therapeutic agent will therefore depend on addressing this knowledge gap. Eugenol initiates increases in cytosolic Ca2+ in Saccharomyces cerevisiae which is partly dependent on the plasma membrane calcium channel, Cch1p. However, it is unclear whether a toxic cytosolic Ca2+elevation mediates the fungicidal activity of eugenol. In the present study, no significant difference in yeast survival was observed following transient eugenol treatment in the presence or absence of extracellular Ca2+. Furthermore, using yeast expressing apoaequorin to report cytosolic Ca2+ and a range of eugenol derivatives, antifungal activity did not appear to be coupled to Ca2+ influx or cytosolic Ca2+ elevation. Taken together, these results suggest that eugenol toxicity is not dependent on a toxic influx of Ca2+. In contrast, careful control of extracellular Ca2+ (using EGTA or BAPTA) revealed that tolerance of yeast to eugenol depended on Ca2+ influx via Cch1p. These findings expose significant differences between the antifungal activity of eugenol and that of azoles, amiodarone and carvacrol. This study highlights the potential to use eugenol in combination with other antifungal agents that exhibit differing modes of action as antifungal agents to combat drug resistant infections.
format	article
author	Stephen K Roberts Martin McAinsh Hanna Cantopher Sean Sandison
author_facet	Stephen K Roberts Martin McAinsh Hanna Cantopher Sean Sandison
author_sort	Stephen K Roberts
title	Calcium dependence of eugenol tolerance and toxicity in Saccharomyces cerevisiae.
title_short	Calcium dependence of eugenol tolerance and toxicity in Saccharomyces cerevisiae.
title_full	Calcium dependence of eugenol tolerance and toxicity in Saccharomyces cerevisiae.
title_fullStr	Calcium dependence of eugenol tolerance and toxicity in Saccharomyces cerevisiae.
title_full_unstemmed	Calcium dependence of eugenol tolerance and toxicity in Saccharomyces cerevisiae.
title_sort	calcium dependence of eugenol tolerance and toxicity in saccharomyces cerevisiae.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/012cd1cf88384a8fa8116bd1d389ce72
work_keys_str_mv	AT stephenkroberts calciumdependenceofeugenoltoleranceandtoxicityinsaccharomycescerevisiae AT martinmcainsh calciumdependenceofeugenoltoleranceandtoxicityinsaccharomycescerevisiae AT hannacantopher calciumdependenceofeugenoltoleranceandtoxicityinsaccharomycescerevisiae AT seansandison calciumdependenceofeugenoltoleranceandtoxicityinsaccharomycescerevisiae
_version_	1718414143280644096

Calcium dependence of eugenol tolerance and toxicity in Saccharomyces cerevisiae.

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