Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions

Abstract The mouse is the most important mammalian model in life science research and the behavior of the mouse is a key read-out of experimental interventions and genetic manipulations. To serve this purpose a solid understanding of the mouse normal behavior is a prerequisite. Using 14–19 months of...

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Autores principales:	Karin Pernold, Eric Rullman, Brun Ulfhake
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0133101a7c794ac68e523eef0dd24c5b
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spelling	oai:doaj.org-article:0133101a7c794ac68e523eef0dd24c5b2021-12-02T13:19:31ZMajor oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions10.1038/s41598-021-84141-92045-2322https://doaj.org/article/0133101a7c794ac68e523eef0dd24c5b2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84141-9https://doaj.org/toc/2045-2322Abstract The mouse is the most important mammalian model in life science research and the behavior of the mouse is a key read-out of experimental interventions and genetic manipulations. To serve this purpose a solid understanding of the mouse normal behavior is a prerequisite. Using 14–19 months of cumulative 24/7 home-cage activity recorded with a non-intrusive technique, evidence is here provided for a highly significant circannual oscillation in spontaneous activity (1–2 SD of the mean, on average 65% higher during peak of highs than lows; P = 7E−50) of male and female C57BL/6 mice held under constant conditions. The periodicity of this hitherto not recognized oscillation is in the range of 2–4 months (average estimate was 97 days across cohorts of cages). It off-sets responses to environmental stimuli and co-varies with the feeding behavior but does not significantly alter the preference for being active during the dark hours. The absence of coordination of this rhythmicity between cages with mice or seasons of the year suggest that the oscillation of physical activity is generated by a free-running intrinsic oscillator devoid of external timer. Due to the magnitude of this rhythmic variation it may be a serious confounder in experiments on mice if left unrecognized.Karin PernoldEric RullmanBrun UlfhakeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karin Pernold Eric Rullman Brun Ulfhake Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions
description	Abstract The mouse is the most important mammalian model in life science research and the behavior of the mouse is a key read-out of experimental interventions and genetic manipulations. To serve this purpose a solid understanding of the mouse normal behavior is a prerequisite. Using 14–19 months of cumulative 24/7 home-cage activity recorded with a non-intrusive technique, evidence is here provided for a highly significant circannual oscillation in spontaneous activity (1–2 SD of the mean, on average 65% higher during peak of highs than lows; P = 7E−50) of male and female C57BL/6 mice held under constant conditions. The periodicity of this hitherto not recognized oscillation is in the range of 2–4 months (average estimate was 97 days across cohorts of cages). It off-sets responses to environmental stimuli and co-varies with the feeding behavior but does not significantly alter the preference for being active during the dark hours. The absence of coordination of this rhythmicity between cages with mice or seasons of the year suggest that the oscillation of physical activity is generated by a free-running intrinsic oscillator devoid of external timer. Due to the magnitude of this rhythmic variation it may be a serious confounder in experiments on mice if left unrecognized.
format	article
author	Karin Pernold Eric Rullman Brun Ulfhake
author_facet	Karin Pernold Eric Rullman Brun Ulfhake
author_sort	Karin Pernold
title	Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions
title_short	Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions
title_full	Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions
title_fullStr	Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions
title_full_unstemmed	Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions
title_sort	major oscillations in spontaneous home-cage activity in c57bl/6 mice housed under constant conditions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0133101a7c794ac68e523eef0dd24c5b
work_keys_str_mv	AT karinpernold majoroscillationsinspontaneoushomecageactivityinc57bl6micehousedunderconstantconditions AT ericrullman majoroscillationsinspontaneoushomecageactivityinc57bl6micehousedunderconstantconditions AT brunulfhake majoroscillationsinspontaneoushomecageactivityinc57bl6micehousedunderconstantconditions
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Major oscillations in spontaneous home-cage activity in C57BL/6 mice housed under constant conditions

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