A Snapshot of the Genetic Diversity of <i>Salmonella</i> Enteritidis Population Involved in Human Infections in Romania Taken in the European Epidemiological Context

In the absence of consistent national molecular typing data to enhance the surveillance of <i>Salmonella</i> Enteritidis, it was considered useful to collect baseline information on the genetic diversity and antibiotic susceptibility of strains isolated in Romania between January 2016 an...

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Autores principales:	Codruta-Romanita Usein, Mihaela Oprea, Adriana Simona Ciontea, Sorin Dinu, Daniela Cristea, Lavinia Cipriana Zota, Saara Kotila
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Salmonella</i> Enteritidis salmonellosis antimicrobial resistance MLVA MLST cgMLST Medicine R
Acceso en línea:	https://doaj.org/article/0134c75747834ced829a8c3ba57a251c
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spelling	oai:doaj.org-article:0134c75747834ced829a8c3ba57a251c2021-11-25T18:38:40ZA Snapshot of the Genetic Diversity of <i>Salmonella</i> Enteritidis Population Involved in Human Infections in Romania Taken in the European Epidemiological Context10.3390/pathogens101114902076-0817https://doaj.org/article/0134c75747834ced829a8c3ba57a251c2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1490https://doaj.org/toc/2076-0817In the absence of consistent national molecular typing data to enhance the surveillance of <i>Salmonella</i> Enteritidis, it was considered useful to collect baseline information on the genetic diversity and antibiotic susceptibility of strains isolated in Romania between January 2016 and April 2020 and compare them to strains described in major international outbreaks of the same period. A collection of 245 clinical isolates were genotyped by a standardised multiple-locus variable-number of tandem repeats analysis (MLVA) 5-loci protocol and screened for antimicrobial resistance against 15 compounds. Twenty strains were further subjected to whole genome sequencing (WGS) and compared to epidemiologically relevant high-throughput sequencing data available in European databases. Twenty-seven MLVA genotypes were identified, of which three, commonly reported in Europe between 2016–2020, covered 72% of the collection. Antibiotic resistance was detected in 30% of the strains, with resistance to nalidixic acid and ciprofloxacin as the most common phenotype, and also associated with two prevalent MLVA clones. WGS-derived multilocus sequence typing (MLST) revealed a single sequence type (ST11) further resolved into 10 core-genome MLST complex types. The minimum spanning tree constructed from the cgMLST data clustered Romanian and international strains, which shared more than 95% of the core genes, revealing links with a contemporaneous multi-country outbreak. This study could be regarded as a forerunner to the advent of using this integrative approach in the public health practice at a national level and thus contribute to the concerted actions at a European level to stop outbreaks.Codruta-Romanita UseinMihaela OpreaAdriana Simona CionteaSorin DinuDaniela CristeaLavinia Cipriana ZotaSaara KotilaMDPI AGarticle<i>Salmonella</i> Enteritidissalmonellosisantimicrobial resistanceMLVAMLSTcgMLSTMedicineRENPathogens, Vol 10, Iss 1490, p 1490 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Salmonella</i> Enteritidis salmonellosis antimicrobial resistance MLVA MLST cgMLST Medicine R
spellingShingle	<i>Salmonella</i> Enteritidis salmonellosis antimicrobial resistance MLVA MLST cgMLST Medicine R Codruta-Romanita Usein Mihaela Oprea Adriana Simona Ciontea Sorin Dinu Daniela Cristea Lavinia Cipriana Zota Saara Kotila A Snapshot of the Genetic Diversity of <i>Salmonella</i> Enteritidis Population Involved in Human Infections in Romania Taken in the European Epidemiological Context
description	In the absence of consistent national molecular typing data to enhance the surveillance of <i>Salmonella</i> Enteritidis, it was considered useful to collect baseline information on the genetic diversity and antibiotic susceptibility of strains isolated in Romania between January 2016 and April 2020 and compare them to strains described in major international outbreaks of the same period. A collection of 245 clinical isolates were genotyped by a standardised multiple-locus variable-number of tandem repeats analysis (MLVA) 5-loci protocol and screened for antimicrobial resistance against 15 compounds. Twenty strains were further subjected to whole genome sequencing (WGS) and compared to epidemiologically relevant high-throughput sequencing data available in European databases. Twenty-seven MLVA genotypes were identified, of which three, commonly reported in Europe between 2016–2020, covered 72% of the collection. Antibiotic resistance was detected in 30% of the strains, with resistance to nalidixic acid and ciprofloxacin as the most common phenotype, and also associated with two prevalent MLVA clones. WGS-derived multilocus sequence typing (MLST) revealed a single sequence type (ST11) further resolved into 10 core-genome MLST complex types. The minimum spanning tree constructed from the cgMLST data clustered Romanian and international strains, which shared more than 95% of the core genes, revealing links with a contemporaneous multi-country outbreak. This study could be regarded as a forerunner to the advent of using this integrative approach in the public health practice at a national level and thus contribute to the concerted actions at a European level to stop outbreaks.
format	article
author	Codruta-Romanita Usein Mihaela Oprea Adriana Simona Ciontea Sorin Dinu Daniela Cristea Lavinia Cipriana Zota Saara Kotila
author_facet	Codruta-Romanita Usein Mihaela Oprea Adriana Simona Ciontea Sorin Dinu Daniela Cristea Lavinia Cipriana Zota Saara Kotila
author_sort	Codruta-Romanita Usein
title	A Snapshot of the Genetic Diversity of <i>Salmonella</i> Enteritidis Population Involved in Human Infections in Romania Taken in the European Epidemiological Context
title_short	A Snapshot of the Genetic Diversity of <i>Salmonella</i> Enteritidis Population Involved in Human Infections in Romania Taken in the European Epidemiological Context
title_full	A Snapshot of the Genetic Diversity of <i>Salmonella</i> Enteritidis Population Involved in Human Infections in Romania Taken in the European Epidemiological Context
title_fullStr	A Snapshot of the Genetic Diversity of <i>Salmonella</i> Enteritidis Population Involved in Human Infections in Romania Taken in the European Epidemiological Context
title_full_unstemmed	A Snapshot of the Genetic Diversity of <i>Salmonella</i> Enteritidis Population Involved in Human Infections in Romania Taken in the European Epidemiological Context
title_sort	snapshot of the genetic diversity of <i>salmonella</i> enteritidis population involved in human infections in romania taken in the european epidemiological context
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0134c75747834ced829a8c3ba57a251c
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A Snapshot of the Genetic Diversity of <i>Salmonella</i> Enteritidis Population Involved in Human Infections in Romania Taken in the European Epidemiological Context

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