Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update

Abstract Background The Borrelia burgdorferi sensu lato (s.l.) genogroup is the causative agent responsible for Lyme borreliosis, a common tick-borne infectious disease in some temperate regions of the Northern Hemisphere. In humans, the clinical manifestations of Lyme borreliosis vary from dermatol...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zsuzsa Kalmár, Violeta Briciu, Mircea Coroian, Mirela Flonta, Amanda-Lelia Rădulescu, Adriana Topan, Andrei Daniel Mihalca, Mihaela Lupșe
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Lyme disease Seroprevalence Borrelia burgdorferi Antibodies Ixodes ricinus Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/0134d86374a24303ae241406afa0126a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0134d86374a24303ae241406afa0126a
record_format	dspace
spelling	oai:doaj.org-article:0134d86374a24303ae241406afa0126a2021-12-05T12:17:02ZSeroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update10.1186/s13071-021-05099-11756-3305https://doaj.org/article/0134d86374a24303ae241406afa0126a2021-12-01T00:00:00Zhttps://doi.org/10.1186/s13071-021-05099-1https://doaj.org/toc/1756-3305Abstract Background The Borrelia burgdorferi sensu lato (s.l.) genogroup is the causative agent responsible for Lyme borreliosis, a common tick-borne infectious disease in some temperate regions of the Northern Hemisphere. In humans, the clinical manifestations of Lyme borreliosis vary from dermatological infection to severe systemic manifestations. In Romania, data on the seroprevalence of Lyme borreliosis and associated risk factors are scarce and outdated, as the only seroprevalence study with a large dataset was published more than 20 years ago. Therefore, the aim of the present study was to evaluate the seroprevalence for Borrelia burgdorferi s.l. in healthy blood donors from six Romanian counties and identify the associated risk factors. Methods The study was conducted among 1200 healthy blood donors aged between 18 and 65 years during November 2019 and September 2020 from six counties in the northwestern and central parts of Romania. A two-tiered testing strategy was applied. Positive and equivocal immunoenzymatic test results for IgG and IgM antibodies were further confirmed by Western blot. Results Serum samples from 20% of the blood donors had positive or equivocal IgG and IgM ELISA index values. In total, 2.3% of the serum samples for IgG and 1.8% for IgM were positive by Western blot. The seroprevalence for both antibodies varied between 1.5% (Satu-Mare) and 6.5% (Bistrița-Năsăud) in the six counties investigated. The highest seroprevalence was observed in men (4.7%), in blood donors performing their professional activities outdoors (4.2%), and in those aged ≥ 56 years (8%). Conclusions These findings confirm the presence of specific IgG and IgM antibodies to B. burgdorferi s.l. among healthy blood donors from Romania. Furthermore, potential risk factors, such as gender, age, and behavior, associated with the presence of positive B. burgdorferi s.l. antibodies among healthy blood donors were identified. Graphical AbstractZsuzsa KalmárVioleta BriciuMircea CoroianMirela FlontaAmanda-Lelia RădulescuAdriana TopanAndrei Daniel MihalcaMihaela LupșeBMCarticleLyme diseaseSeroprevalenceBorrelia burgdorferiAntibodiesIxodes ricinusInfectious and parasitic diseasesRC109-216ENParasites & Vectors, Vol 14, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lyme disease Seroprevalence Borrelia burgdorferi Antibodies Ixodes ricinus Infectious and parasitic diseases RC109-216
spellingShingle	Lyme disease Seroprevalence Borrelia burgdorferi Antibodies Ixodes ricinus Infectious and parasitic diseases RC109-216 Zsuzsa Kalmár Violeta Briciu Mircea Coroian Mirela Flonta Amanda-Lelia Rădulescu Adriana Topan Andrei Daniel Mihalca Mihaela Lupșe Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update
description	Abstract Background The Borrelia burgdorferi sensu lato (s.l.) genogroup is the causative agent responsible for Lyme borreliosis, a common tick-borne infectious disease in some temperate regions of the Northern Hemisphere. In humans, the clinical manifestations of Lyme borreliosis vary from dermatological infection to severe systemic manifestations. In Romania, data on the seroprevalence of Lyme borreliosis and associated risk factors are scarce and outdated, as the only seroprevalence study with a large dataset was published more than 20 years ago. Therefore, the aim of the present study was to evaluate the seroprevalence for Borrelia burgdorferi s.l. in healthy blood donors from six Romanian counties and identify the associated risk factors. Methods The study was conducted among 1200 healthy blood donors aged between 18 and 65 years during November 2019 and September 2020 from six counties in the northwestern and central parts of Romania. A two-tiered testing strategy was applied. Positive and equivocal immunoenzymatic test results for IgG and IgM antibodies were further confirmed by Western blot. Results Serum samples from 20% of the blood donors had positive or equivocal IgG and IgM ELISA index values. In total, 2.3% of the serum samples for IgG and 1.8% for IgM were positive by Western blot. The seroprevalence for both antibodies varied between 1.5% (Satu-Mare) and 6.5% (Bistrița-Năsăud) in the six counties investigated. The highest seroprevalence was observed in men (4.7%), in blood donors performing their professional activities outdoors (4.2%), and in those aged ≥ 56 years (8%). Conclusions These findings confirm the presence of specific IgG and IgM antibodies to B. burgdorferi s.l. among healthy blood donors from Romania. Furthermore, potential risk factors, such as gender, age, and behavior, associated with the presence of positive B. burgdorferi s.l. antibodies among healthy blood donors were identified. Graphical Abstract
format	article
author	Zsuzsa Kalmár Violeta Briciu Mircea Coroian Mirela Flonta Amanda-Lelia Rădulescu Adriana Topan Andrei Daniel Mihalca Mihaela Lupșe
author_facet	Zsuzsa Kalmár Violeta Briciu Mircea Coroian Mirela Flonta Amanda-Lelia Rădulescu Adriana Topan Andrei Daniel Mihalca Mihaela Lupșe
author_sort	Zsuzsa Kalmár
title	Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update
title_short	Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update
title_full	Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update
title_fullStr	Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update
title_full_unstemmed	Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update
title_sort	seroprevalence of antibodies against borrelia burgdorferi sensu lato in healthy blood donors in romania: an update
publisher	BMC
publishDate	2021
url	https://doaj.org/article/0134d86374a24303ae241406afa0126a
work_keys_str_mv	AT zsuzsakalmar seroprevalenceofantibodiesagainstborreliaburgdorferisensulatoinhealthyblooddonorsinromaniaanupdate AT violetabriciu seroprevalenceofantibodiesagainstborreliaburgdorferisensulatoinhealthyblooddonorsinromaniaanupdate AT mirceacoroian seroprevalenceofantibodiesagainstborreliaburgdorferisensulatoinhealthyblooddonorsinromaniaanupdate AT mirelaflonta seroprevalenceofantibodiesagainstborreliaburgdorferisensulatoinhealthyblooddonorsinromaniaanupdate AT amandaleliaradulescu seroprevalenceofantibodiesagainstborreliaburgdorferisensulatoinhealthyblooddonorsinromaniaanupdate AT adrianatopan seroprevalenceofantibodiesagainstborreliaburgdorferisensulatoinhealthyblooddonorsinromaniaanupdate AT andreidanielmihalca seroprevalenceofantibodiesagainstborreliaburgdorferisensulatoinhealthyblooddonorsinromaniaanupdate AT mihaelalupse seroprevalenceofantibodiesagainstborreliaburgdorferisensulatoinhealthyblooddonorsinromaniaanupdate
_version_	1718372055250894848

Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update

Ejemplares similares