Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.

Aging in organisms as diverse as yeast, nematodes, and mammals is delayed by caloric restriction, an effect mediated by the nutrient sensing TOR, RAS/cAMP, and AKT/Sch9 pathways. The transcription factor Gis1 functions downstream of these pathways in extending the lifespan of nutrient restricted yea...

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Autores principales:	Jakub Orzechowski Westholm, Susanna Tronnersjö, Niklas Nordberg, Ida Olsson, Jan Komorowski, Hans Ronne
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/01445a9169cc42c5a7c83deb59272869
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spelling	oai:doaj.org-article:01445a9169cc42c5a7c83deb592728692021-11-18T07:27:19ZGis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.1932-620310.1371/journal.pone.0031577https://doaj.org/article/01445a9169cc42c5a7c83deb592728692012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22363679/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Aging in organisms as diverse as yeast, nematodes, and mammals is delayed by caloric restriction, an effect mediated by the nutrient sensing TOR, RAS/cAMP, and AKT/Sch9 pathways. The transcription factor Gis1 functions downstream of these pathways in extending the lifespan of nutrient restricted yeast cells, but the mechanisms involved are still poorly understood. We have used gene expression microarrays to study the targets of Gis1 and the related protein Rph1 in different growth phases. Our results show that Gis1 and Rph1 act both as repressors and activators, on overlapping sets of genes as well as on distinct targets. Interestingly, both the activities and the target specificities of Gis1 and Rph1 depend on the growth phase. Thus, both proteins are associated with repression during exponential growth, targeting genes with STRE or PDS motifs in their promoters. After the diauxic shift, both become involved in activation, with Gis1 acting primarily on genes with PDS motifs, and Rph1 on genes with STRE motifs. Significantly, Gis1 and Rph1 control a number of genes involved in acetate and glycerol formation, metabolites that have been implicated in aging. Furthermore, several genes involved in acetyl-CoA metabolism are downregulated by Gis1.Jakub Orzechowski WestholmSusanna TronnersjöNiklas NordbergIda OlssonJan KomorowskiHans RonnePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31577 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jakub Orzechowski Westholm Susanna Tronnersjö Niklas Nordberg Ida Olsson Jan Komorowski Hans Ronne Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.
description	Aging in organisms as diverse as yeast, nematodes, and mammals is delayed by caloric restriction, an effect mediated by the nutrient sensing TOR, RAS/cAMP, and AKT/Sch9 pathways. The transcription factor Gis1 functions downstream of these pathways in extending the lifespan of nutrient restricted yeast cells, but the mechanisms involved are still poorly understood. We have used gene expression microarrays to study the targets of Gis1 and the related protein Rph1 in different growth phases. Our results show that Gis1 and Rph1 act both as repressors and activators, on overlapping sets of genes as well as on distinct targets. Interestingly, both the activities and the target specificities of Gis1 and Rph1 depend on the growth phase. Thus, both proteins are associated with repression during exponential growth, targeting genes with STRE or PDS motifs in their promoters. After the diauxic shift, both become involved in activation, with Gis1 acting primarily on genes with PDS motifs, and Rph1 on genes with STRE motifs. Significantly, Gis1 and Rph1 control a number of genes involved in acetate and glycerol formation, metabolites that have been implicated in aging. Furthermore, several genes involved in acetyl-CoA metabolism are downregulated by Gis1.
format	article
author	Jakub Orzechowski Westholm Susanna Tronnersjö Niklas Nordberg Ida Olsson Jan Komorowski Hans Ronne
author_facet	Jakub Orzechowski Westholm Susanna Tronnersjö Niklas Nordberg Ida Olsson Jan Komorowski Hans Ronne
author_sort	Jakub Orzechowski Westholm
title	Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.
title_short	Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.
title_full	Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.
title_fullStr	Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.
title_full_unstemmed	Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.
title_sort	gis1 and rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/01445a9169cc42c5a7c83deb59272869
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Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.

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