Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves

Abstract Understanding how nerves spontaneously innervate tissues or regenerate small injuries is critical to enhance material-based interventions to regenerate large scale, traumatic injuries. During embryogenesis, neural and vascular tissues form interconnected, complex networks as a result of sig...

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Autores principales:	Jonathan M. Grasman, David L. Kaplan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0152d54b71804989979bc1359d321c67
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spelling	oai:doaj.org-article:0152d54b71804989979bc1359d321c672021-12-02T15:05:33ZHuman endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves10.1038/s41598-017-04460-82045-2322https://doaj.org/article/0152d54b71804989979bc1359d321c672017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04460-8https://doaj.org/toc/2045-2322Abstract Understanding how nerves spontaneously innervate tissues or regenerate small injuries is critical to enhance material-based interventions to regenerate large scale, traumatic injuries. During embryogenesis, neural and vascular tissues form interconnected, complex networks as a result of signaling between these tissue types. Here, we report that human endothelial cells (HUVECs) secrete brain-derived neurotrophic factor (BDNF), which significantly stimulated axonal growth from chicken or rat dorsal root ganglia (DRGs). HUVEC-conditioned medium was sufficient to enhance axonal growth, demonstrating that direct cell-cell contact was not required. When BDNF was neutralized, there was a significant reduction in axonal growth when incubated in HUVEC-conditioned medium and in direct co-culture with HUVECs. These data show that HUVECs secrete neurotrophic factors that significantly enhance axonal growth, and can inform future in vivo studies to direct or pattern the angiogenic response in regenerating tissues to encourage re-innervation.Jonathan M. GrasmanDavid L. KaplanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jonathan M. Grasman David L. Kaplan Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves
description	Abstract Understanding how nerves spontaneously innervate tissues or regenerate small injuries is critical to enhance material-based interventions to regenerate large scale, traumatic injuries. During embryogenesis, neural and vascular tissues form interconnected, complex networks as a result of signaling between these tissue types. Here, we report that human endothelial cells (HUVECs) secrete brain-derived neurotrophic factor (BDNF), which significantly stimulated axonal growth from chicken or rat dorsal root ganglia (DRGs). HUVEC-conditioned medium was sufficient to enhance axonal growth, demonstrating that direct cell-cell contact was not required. When BDNF was neutralized, there was a significant reduction in axonal growth when incubated in HUVEC-conditioned medium and in direct co-culture with HUVECs. These data show that HUVECs secrete neurotrophic factors that significantly enhance axonal growth, and can inform future in vivo studies to direct or pattern the angiogenic response in regenerating tissues to encourage re-innervation.
format	article
author	Jonathan M. Grasman David L. Kaplan
author_facet	Jonathan M. Grasman David L. Kaplan
author_sort	Jonathan M. Grasman
title	Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves
title_short	Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves
title_full	Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves
title_fullStr	Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves
title_full_unstemmed	Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves
title_sort	human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0152d54b71804989979bc1359d321c67
work_keys_str_mv	AT jonathanmgrasman humanendothelialcellssecreteneurotropicfactorstodirectaxonalgrowthofperipheralnerves AT davidlkaplan humanendothelialcellssecreteneurotropicfactorstodirectaxonalgrowthofperipheralnerves
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Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves

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