Genomic Identification and Functional Analysis of JHAMTs in the Pond Wolf Spider, <i>Pardosa pseudoannulata</i>

Juvenile hormone (JH) plays a critical role in many physiological activities of Arthropoda. Juvenile hormone acid methyltransferase (JHAMT) is involved in the last steps of JH biosynthesis as an important rate-limiting enzyme. In recent studies, an increasing number of JHAMTs were identified in arth...

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Autores principales:	Zhi-Ming Yang, Yong Wu, Fang-Fang Li, Zhang-Jin Zhou, Na Yu, Ze-Wen Liu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Pardosa pseudoannulata</i> JHAMT phylogeny RNA interference gene expansion Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/01570e5761984392a5bb3e405206c74e
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spelling	oai:doaj.org-article:01570e5761984392a5bb3e405206c74e2021-11-11T17:10:40ZGenomic Identification and Functional Analysis of JHAMTs in the Pond Wolf Spider, <i>Pardosa pseudoannulata</i>10.3390/ijms2221117211422-00671661-6596https://doaj.org/article/01570e5761984392a5bb3e405206c74e2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11721https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Juvenile hormone (JH) plays a critical role in many physiological activities of Arthropoda. Juvenile hormone acid methyltransferase (JHAMT) is involved in the last steps of JH biosynthesis as an important rate-limiting enzyme. In recent studies, an increasing number of JHAMTs were identified in arthropods, but no JHAMT was reported in spiders. Herein, eight JHAMTs were identified in the pond wolf spider, <i>Pardosa pseudoannulata</i>, all containing the well conserved S-adenosyl-L-methionine binding motif. <i>JHAMT-1</i> and the other seven <i>JHAMTs</i> were located at chromosome 13 and chromosome 1, respectively. Multiple alignment and phylogenetic analysis showed that JHAMT-1 was grouped together with insect JHAMTs independently and shared high similarities with insect JHAMTs compared to the other seven JHAMTs. In addition, <i>JHAMT-1</i>, <i>JHAMT-2</i>, and <i>JHAMT-3</i> were highly expressed in the abdomen of spiderlings and could respond to the stimulation of exogenous farnesoic acid. Meanwhile, knockdown of these three <i>JHAMTs</i> caused the overweight and accelerated molting of spiderlings. These results demonstrated the cooperation of multi-JHAMTs in spider development and provided a new evolutionary perspective of the expansion of <i>JHAMT</i> in Arachnida.Zhi-Ming YangYong WuFang-Fang LiZhang-Jin ZhouNa YuZe-Wen LiuMDPI AGarticle<i>Pardosa pseudoannulata</i>JHAMTphylogenyRNA interferencegene expansionBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11721, p 11721 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Pardosa pseudoannulata</i> JHAMT phylogeny RNA interference gene expansion Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Pardosa pseudoannulata</i> JHAMT phylogeny RNA interference gene expansion Biology (General) QH301-705.5 Chemistry QD1-999 Zhi-Ming Yang Yong Wu Fang-Fang Li Zhang-Jin Zhou Na Yu Ze-Wen Liu Genomic Identification and Functional Analysis of JHAMTs in the Pond Wolf Spider, <i>Pardosa pseudoannulata</i>
description	Juvenile hormone (JH) plays a critical role in many physiological activities of Arthropoda. Juvenile hormone acid methyltransferase (JHAMT) is involved in the last steps of JH biosynthesis as an important rate-limiting enzyme. In recent studies, an increasing number of JHAMTs were identified in arthropods, but no JHAMT was reported in spiders. Herein, eight JHAMTs were identified in the pond wolf spider, <i>Pardosa pseudoannulata</i>, all containing the well conserved S-adenosyl-L-methionine binding motif. <i>JHAMT-1</i> and the other seven <i>JHAMTs</i> were located at chromosome 13 and chromosome 1, respectively. Multiple alignment and phylogenetic analysis showed that JHAMT-1 was grouped together with insect JHAMTs independently and shared high similarities with insect JHAMTs compared to the other seven JHAMTs. In addition, <i>JHAMT-1</i>, <i>JHAMT-2</i>, and <i>JHAMT-3</i> were highly expressed in the abdomen of spiderlings and could respond to the stimulation of exogenous farnesoic acid. Meanwhile, knockdown of these three <i>JHAMTs</i> caused the overweight and accelerated molting of spiderlings. These results demonstrated the cooperation of multi-JHAMTs in spider development and provided a new evolutionary perspective of the expansion of <i>JHAMT</i> in Arachnida.
format	article
author	Zhi-Ming Yang Yong Wu Fang-Fang Li Zhang-Jin Zhou Na Yu Ze-Wen Liu
author_facet	Zhi-Ming Yang Yong Wu Fang-Fang Li Zhang-Jin Zhou Na Yu Ze-Wen Liu
author_sort	Zhi-Ming Yang
title	Genomic Identification and Functional Analysis of JHAMTs in the Pond Wolf Spider, <i>Pardosa pseudoannulata</i>
title_short	Genomic Identification and Functional Analysis of JHAMTs in the Pond Wolf Spider, <i>Pardosa pseudoannulata</i>
title_full	Genomic Identification and Functional Analysis of JHAMTs in the Pond Wolf Spider, <i>Pardosa pseudoannulata</i>
title_fullStr	Genomic Identification and Functional Analysis of JHAMTs in the Pond Wolf Spider, <i>Pardosa pseudoannulata</i>
title_full_unstemmed	Genomic Identification and Functional Analysis of JHAMTs in the Pond Wolf Spider, <i>Pardosa pseudoannulata</i>
title_sort	genomic identification and functional analysis of jhamts in the pond wolf spider, <i>pardosa pseudoannulata</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/01570e5761984392a5bb3e405206c74e
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Genomic Identification and Functional Analysis of JHAMTs in the Pond Wolf Spider, <i>Pardosa pseudoannulata</i>

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