Evaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes.

Whereas it is evident that a well aligned and regular sarcomeric structure in cardiomyocytes is vital for heart function, considerably less is known about the contribution of individual elements to the mechanics of the entire cell. For instance, it is unclear whether altered Z-disc elements are the...

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Autores principales:	Dominik Müller, Thorben Klamt, Lara Gentemann, Alexander Heisterkamp, Stefan Michael Klaus Kalies
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0158c4ce262b4fc39713f32ad846dde8
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spelling	oai:doaj.org-article:0158c4ce262b4fc39713f32ad846dde82021-11-25T06:23:35ZEvaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes.1932-620310.1371/journal.pone.0252346https://doaj.org/article/0158c4ce262b4fc39713f32ad846dde82021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252346https://doaj.org/toc/1932-6203Whereas it is evident that a well aligned and regular sarcomeric structure in cardiomyocytes is vital for heart function, considerably less is known about the contribution of individual elements to the mechanics of the entire cell. For instance, it is unclear whether altered Z-disc elements are the reason or the outcome of related cardiomyopathies. Therefore, it is crucial to gain more insight into this cellular organization. This study utilizes femtosecond laser-based nanosurgery to better understand sarcomeres and their repair upon damage. We investigated the influence of the extent and the location of the Z-disc damage. A single, three, five or ten Z-disc ablations were performed in neonatal rat cardiomyocytes. We employed image-based analysis using a self-written software together with different already published algorithms. We observed that cardiomyocyte survival associated with the damage extent, but not with the cell area or the total number of Z-discs per cell. The cell survival is independent of the damage position and can be compensated. However, the sarcomere alignment/orientation is changing over time after ablation. The contraction time is also independent of the extent of damage for the tested parameters. Additionally, we observed shortening rates between 6-7% of the initial sarcomere length in laser treated cardiomyocytes. This rate is an important indicator for force generation in myocytes. In conclusion, femtosecond laser-based nanosurgery together with image-based sarcomere tracking is a powerful tool to better understand the Z-disc complex and its force propagation function and role in cellular mechanisms.Dominik MüllerThorben KlamtLara GentemannAlexander HeisterkampStefan Michael Klaus KaliesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252346 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dominik Müller Thorben Klamt Lara Gentemann Alexander Heisterkamp Stefan Michael Klaus Kalies Evaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes.
description	Whereas it is evident that a well aligned and regular sarcomeric structure in cardiomyocytes is vital for heart function, considerably less is known about the contribution of individual elements to the mechanics of the entire cell. For instance, it is unclear whether altered Z-disc elements are the reason or the outcome of related cardiomyopathies. Therefore, it is crucial to gain more insight into this cellular organization. This study utilizes femtosecond laser-based nanosurgery to better understand sarcomeres and their repair upon damage. We investigated the influence of the extent and the location of the Z-disc damage. A single, three, five or ten Z-disc ablations were performed in neonatal rat cardiomyocytes. We employed image-based analysis using a self-written software together with different already published algorithms. We observed that cardiomyocyte survival associated with the damage extent, but not with the cell area or the total number of Z-discs per cell. The cell survival is independent of the damage position and can be compensated. However, the sarcomere alignment/orientation is changing over time after ablation. The contraction time is also independent of the extent of damage for the tested parameters. Additionally, we observed shortening rates between 6-7% of the initial sarcomere length in laser treated cardiomyocytes. This rate is an important indicator for force generation in myocytes. In conclusion, femtosecond laser-based nanosurgery together with image-based sarcomere tracking is a powerful tool to better understand the Z-disc complex and its force propagation function and role in cellular mechanisms.
format	article
author	Dominik Müller Thorben Klamt Lara Gentemann Alexander Heisterkamp Stefan Michael Klaus Kalies
author_facet	Dominik Müller Thorben Klamt Lara Gentemann Alexander Heisterkamp Stefan Michael Klaus Kalies
author_sort	Dominik Müller
title	Evaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes.
title_short	Evaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes.
title_full	Evaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes.
title_fullStr	Evaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes.
title_full_unstemmed	Evaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes.
title_sort	evaluation of laser induced sarcomere micro-damage: role of damage extent and location in cardiomyocytes.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0158c4ce262b4fc39713f32ad846dde8
work_keys_str_mv	AT dominikmuller evaluationoflaserinducedsarcomeremicrodamageroleofdamageextentandlocationincardiomyocytes AT thorbenklamt evaluationoflaserinducedsarcomeremicrodamageroleofdamageextentandlocationincardiomyocytes AT laragentemann evaluationoflaserinducedsarcomeremicrodamageroleofdamageextentandlocationincardiomyocytes AT alexanderheisterkamp evaluationoflaserinducedsarcomeremicrodamageroleofdamageextentandlocationincardiomyocytes AT stefanmichaelklauskalies evaluationoflaserinducedsarcomeremicrodamageroleofdamageextentandlocationincardiomyocytes
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Evaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes.

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