BrainPhys neuronal medium optimized for imaging and optogenetics in vitro

BrainPhys neuronal medium optimized for imaging and optogenetics in vitro

Current media for neuronal cell and organoid cultures are suboptimal for functional imaging and optogenetics experiments, owing to phototoxicity and unphysiological performance. Here the authors formulate an optimised neuronal medium to support live cell imaging and electrophysiological activity.

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Detalles Bibliográficos
Autores principales: Michael Zabolocki, Kasandra McCormack, Mark van den Hurk, Bridget Milky, Andrew P. Shoubridge, Robert Adams, Jenne Tran, Anita Mahadevan-Jansen, Philipp Reineck, Jacob Thomas, Mark R. Hutchinson, Carmen K. H. Mak, Adam Añonuevo, Leon H. Chew, Adam J. Hirst, Vivian M. Lee, Erin Knock, Cedric Bardy
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Science
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Acceso en línea:https://doaj.org/article/015ec3ef1ee04a14934cb7a0fabc0fe7
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Descripción
Sumario:Current media for neuronal cell and organoid cultures are suboptimal for functional imaging and optogenetics experiments, owing to phototoxicity and unphysiological performance. Here the authors formulate an optimised neuronal medium to support live cell imaging and electrophysiological activity.

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