BrainPhys neuronal medium optimized for imaging and optogenetics in vitro

BrainPhys neuronal medium optimized for imaging and optogenetics in vitro

Current media for neuronal cell and organoid cultures are suboptimal for functional imaging and optogenetics experiments, owing to phototoxicity and unphysiological performance. Here the authors formulate an optimised neuronal medium to support live cell imaging and electrophysiological activity.

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Bibliographic Details
Main Authors: Michael Zabolocki, Kasandra McCormack, Mark van den Hurk, Bridget Milky, Andrew P. Shoubridge, Robert Adams, Jenne Tran, Anita Mahadevan-Jansen, Philipp Reineck, Jacob Thomas, Mark R. Hutchinson, Carmen K. H. Mak, Adam Añonuevo, Leon H. Chew, Adam J. Hirst, Vivian M. Lee, Erin Knock, Cedric Bardy
Format: article
Language:EN
Published: Nature Portfolio 2020
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Online Access:https://doaj.org/article/015ec3ef1ee04a14934cb7a0fabc0fe7
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Summary:Current media for neuronal cell and organoid cultures are suboptimal for functional imaging and optogenetics experiments, owing to phototoxicity and unphysiological performance. Here the authors formulate an optimised neuronal medium to support live cell imaging and electrophysiological activity.

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