BrainPhys neuronal medium optimized for imaging and optogenetics in vitro

Current media for neuronal cell and organoid cultures are suboptimal for functional imaging and optogenetics experiments, owing to phototoxicity and unphysiological performance. Here the authors formulate an optimised neuronal medium to support live cell imaging and electrophysiological activity.

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Autores principales:	Michael Zabolocki, Kasandra McCormack, Mark van den Hurk, Bridget Milky, Andrew P. Shoubridge, Robert Adams, Jenne Tran, Anita Mahadevan-Jansen, Philipp Reineck, Jacob Thomas, Mark R. Hutchinson, Carmen K. H. Mak, Adam Añonuevo, Leon H. Chew, Adam J. Hirst, Vivian M. Lee, Erin Knock, Cedric Bardy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/015ec3ef1ee04a14934cb7a0fabc0fe7
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spelling	oai:doaj.org-article:015ec3ef1ee04a14934cb7a0fabc0fe72021-12-02T17:31:23ZBrainPhys neuronal medium optimized for imaging and optogenetics in vitro10.1038/s41467-020-19275-x2041-1723https://doaj.org/article/015ec3ef1ee04a14934cb7a0fabc0fe72020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19275-xhttps://doaj.org/toc/2041-1723Current media for neuronal cell and organoid cultures are suboptimal for functional imaging and optogenetics experiments, owing to phototoxicity and unphysiological performance. Here the authors formulate an optimised neuronal medium to support live cell imaging and electrophysiological activity.Michael ZabolockiKasandra McCormackMark van den HurkBridget MilkyAndrew P. ShoubridgeRobert AdamsJenne TranAnita Mahadevan-JansenPhilipp ReineckJacob ThomasMark R. HutchinsonCarmen K. H. MakAdam AñonuevoLeon H. ChewAdam J. HirstVivian M. LeeErin KnockCedric BardyNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-19 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Michael Zabolocki Kasandra McCormack Mark van den Hurk Bridget Milky Andrew P. Shoubridge Robert Adams Jenne Tran Anita Mahadevan-Jansen Philipp Reineck Jacob Thomas Mark R. Hutchinson Carmen K. H. Mak Adam Añonuevo Leon H. Chew Adam J. Hirst Vivian M. Lee Erin Knock Cedric Bardy BrainPhys neuronal medium optimized for imaging and optogenetics in vitro
description	Current media for neuronal cell and organoid cultures are suboptimal for functional imaging and optogenetics experiments, owing to phototoxicity and unphysiological performance. Here the authors formulate an optimised neuronal medium to support live cell imaging and electrophysiological activity.
format	article
author	Michael Zabolocki Kasandra McCormack Mark van den Hurk Bridget Milky Andrew P. Shoubridge Robert Adams Jenne Tran Anita Mahadevan-Jansen Philipp Reineck Jacob Thomas Mark R. Hutchinson Carmen K. H. Mak Adam Añonuevo Leon H. Chew Adam J. Hirst Vivian M. Lee Erin Knock Cedric Bardy
author_facet	Michael Zabolocki Kasandra McCormack Mark van den Hurk Bridget Milky Andrew P. Shoubridge Robert Adams Jenne Tran Anita Mahadevan-Jansen Philipp Reineck Jacob Thomas Mark R. Hutchinson Carmen K. H. Mak Adam Añonuevo Leon H. Chew Adam J. Hirst Vivian M. Lee Erin Knock Cedric Bardy
author_sort	Michael Zabolocki
title	BrainPhys neuronal medium optimized for imaging and optogenetics in vitro
title_short	BrainPhys neuronal medium optimized for imaging and optogenetics in vitro
title_full	BrainPhys neuronal medium optimized for imaging and optogenetics in vitro
title_fullStr	BrainPhys neuronal medium optimized for imaging and optogenetics in vitro
title_full_unstemmed	BrainPhys neuronal medium optimized for imaging and optogenetics in vitro
title_sort	brainphys neuronal medium optimized for imaging and optogenetics in vitro
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/015ec3ef1ee04a14934cb7a0fabc0fe7
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BrainPhys neuronal medium optimized for imaging and optogenetics in vitro

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