Development and Optimization of Solanum Lycocarpum Polyphenol Oxidase-Based Biosensor and Application towards Paracetamol Detection

Purpose: The development biosensing technologies capable of delivering fast and reliable analysis is a growing trend in drug quality control. Considering the emerging use of plant-based polyphenol oxidases (PPO) as biological component of electrochemical biosensors, this work reports the first Solan...

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Autores principales:	Rafael Souza Antunes, Douglas Vieira Thomaz, Luane Ferreira Garcia, Eric de Souza Gil, Flavio Marques Lopes
Formato:	article
Lenguaje:	EN
Publicado:	Tabriz University of Medical Sciences 2021
Materias:	phenolic drugs biosensing technology enzymatic extract carbon paste electrode vegetal enzyme Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/0161013a61924da59588a7bb48713df9
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spelling	oai:doaj.org-article:0161013a61924da59588a7bb48713df92021-12-05T09:40:32ZDevelopment and Optimization of Solanum Lycocarpum Polyphenol Oxidase-Based Biosensor and Application towards Paracetamol Detection2228-58812251-730810.34172/apb.2021.054https://doaj.org/article/0161013a61924da59588a7bb48713df92021-06-01T00:00:00Zhttps://apb.tbzmed.ac.ir/PDF/apb-11-469.pdfhttps://doaj.org/toc/2228-5881https://doaj.org/toc/2251-7308Purpose: The development biosensing technologies capable of delivering fast and reliable analysis is a growing trend in drug quality control. Considering the emerging use of plant-based polyphenol oxidases (PPO) as biological component of electrochemical biosensors, this work reports the first Solanum lycocarpum PPO biosensor and its use in the pharmaceutical analysis of paracetamol in tablet formulations. Methods: The biosensor was optimized regarding fruit maturation (immature and mature-ripe), vegetal extract volume to be used in biosensor construction as well as optimal pH of electrochemical cell fluid. Results: Results evidenced that the extract which rendered the biosensor with best analytical performance was from immature fruits, and the biosensor produced using 100 µL of crude plant extract promoted better faradaic signal gathering. Moreover, when neutral pH media was used in the electrochemical cell, the biosensor showcased best faradaic signal output from the used redox probe (catechol), suggesting thence that the method presents high sensibility for phenolic compounds detection. Furthermore, the biosensor was able to quantify paracetamol in a linear range from 50 to 300 μM, showcasing LoD and LoQ of 3 μM and 10 μM, respectively. Conclusion: after careful evaluation, this biosensor might be a low-cost alternative for conventional pharmaceutical quality control methods.Rafael Souza AntunesDouglas Vieira ThomazLuane Ferreira GarciaEric de Souza GilFlavio Marques LopesTabriz University of Medical Sciences articlephenolic drugsbiosensing technologyenzymatic extractcarbon paste electrodevegetal enzymeTherapeutics. PharmacologyRM1-950ENAdvanced Pharmaceutical Bulletin, Vol 11, Iss 3, Pp 469-476 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	phenolic drugs biosensing technology enzymatic extract carbon paste electrode vegetal enzyme Therapeutics. Pharmacology RM1-950
spellingShingle	phenolic drugs biosensing technology enzymatic extract carbon paste electrode vegetal enzyme Therapeutics. Pharmacology RM1-950 Rafael Souza Antunes Douglas Vieira Thomaz Luane Ferreira Garcia Eric de Souza Gil Flavio Marques Lopes Development and Optimization of Solanum Lycocarpum Polyphenol Oxidase-Based Biosensor and Application towards Paracetamol Detection
description	Purpose: The development biosensing technologies capable of delivering fast and reliable analysis is a growing trend in drug quality control. Considering the emerging use of plant-based polyphenol oxidases (PPO) as biological component of electrochemical biosensors, this work reports the first Solanum lycocarpum PPO biosensor and its use in the pharmaceutical analysis of paracetamol in tablet formulations. Methods: The biosensor was optimized regarding fruit maturation (immature and mature-ripe), vegetal extract volume to be used in biosensor construction as well as optimal pH of electrochemical cell fluid. Results: Results evidenced that the extract which rendered the biosensor with best analytical performance was from immature fruits, and the biosensor produced using 100 µL of crude plant extract promoted better faradaic signal gathering. Moreover, when neutral pH media was used in the electrochemical cell, the biosensor showcased best faradaic signal output from the used redox probe (catechol), suggesting thence that the method presents high sensibility for phenolic compounds detection. Furthermore, the biosensor was able to quantify paracetamol in a linear range from 50 to 300 μM, showcasing LoD and LoQ of 3 μM and 10 μM, respectively. Conclusion: after careful evaluation, this biosensor might be a low-cost alternative for conventional pharmaceutical quality control methods.
format	article
author	Rafael Souza Antunes Douglas Vieira Thomaz Luane Ferreira Garcia Eric de Souza Gil Flavio Marques Lopes
author_facet	Rafael Souza Antunes Douglas Vieira Thomaz Luane Ferreira Garcia Eric de Souza Gil Flavio Marques Lopes
author_sort	Rafael Souza Antunes
title	Development and Optimization of Solanum Lycocarpum Polyphenol Oxidase-Based Biosensor and Application towards Paracetamol Detection
title_short	Development and Optimization of Solanum Lycocarpum Polyphenol Oxidase-Based Biosensor and Application towards Paracetamol Detection
title_full	Development and Optimization of Solanum Lycocarpum Polyphenol Oxidase-Based Biosensor and Application towards Paracetamol Detection
title_fullStr	Development and Optimization of Solanum Lycocarpum Polyphenol Oxidase-Based Biosensor and Application towards Paracetamol Detection
title_full_unstemmed	Development and Optimization of Solanum Lycocarpum Polyphenol Oxidase-Based Biosensor and Application towards Paracetamol Detection
title_sort	development and optimization of solanum lycocarpum polyphenol oxidase-based biosensor and application towards paracetamol detection
publisher	Tabriz University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/0161013a61924da59588a7bb48713df9
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Development and Optimization of Solanum Lycocarpum Polyphenol Oxidase-Based Biosensor and Application towards Paracetamol Detection

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