Induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties.

Induced endothelial cells (iECs) generated from neonatal fibroblasts via transdifferentiation have been shown to have pro-angiogenic properties and are a potential therapy for peripheral arterial disease (PAD). It is unknown if iECs can be generated from fibroblasts collected from PAD patients and w...

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Autores principales:	Jack D Hywood, Sara Sadeghipour, Zoe E Clayton, Jun Yuan, Colleen Stubbs, Jack W T Wong, John P Cooke, Sanjay Patel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0161145f479244b5965f069930afa8fb
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spelling	oai:doaj.org-article:0161145f479244b5965f069930afa8fb2021-12-02T20:15:05ZInduced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties.1932-620310.1371/journal.pone.0255075https://doaj.org/article/0161145f479244b5965f069930afa8fb2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255075https://doaj.org/toc/1932-6203Induced endothelial cells (iECs) generated from neonatal fibroblasts via transdifferentiation have been shown to have pro-angiogenic properties and are a potential therapy for peripheral arterial disease (PAD). It is unknown if iECs can be generated from fibroblasts collected from PAD patients and whether these cells are pro-angiogenic. In this study fibroblasts were collected from four PAD patients undergoing carotid endarterectomies. These cells, and neonatal fibroblasts, were transdifferentiated into iECs using modified mRNA. Endothelial phenotype and pro-angiogenic cytokine secretion were investigated. NOD-SCID mice underwent surgery to induce hindlimb ischaemia in a murine model of PAD. Mice received intramuscular injections with either control vehicle, or 1 × 106 neonatal-derived or 1 × 106 patient-derived iECs. Recovery in perfusion to the affected limb was measured using laser Doppler scanning. Perfusion recovery was enhanced in mice treated with neonatal-derived iECs and in two of the three patient-derived iEC lines investigated in vivo. Patient-derived iECs can be successfully generated from PAD patients and for specific patients display comparable pro-angiogenic properties to neonatal-derived iECs.Jack D HywoodSara SadeghipourZoe E ClaytonJun YuanColleen StubbsJack W T WongJohn P CookeSanjay PatelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255075 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jack D Hywood Sara Sadeghipour Zoe E Clayton Jun Yuan Colleen Stubbs Jack W T Wong John P Cooke Sanjay Patel Induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties.
description	Induced endothelial cells (iECs) generated from neonatal fibroblasts via transdifferentiation have been shown to have pro-angiogenic properties and are a potential therapy for peripheral arterial disease (PAD). It is unknown if iECs can be generated from fibroblasts collected from PAD patients and whether these cells are pro-angiogenic. In this study fibroblasts were collected from four PAD patients undergoing carotid endarterectomies. These cells, and neonatal fibroblasts, were transdifferentiated into iECs using modified mRNA. Endothelial phenotype and pro-angiogenic cytokine secretion were investigated. NOD-SCID mice underwent surgery to induce hindlimb ischaemia in a murine model of PAD. Mice received intramuscular injections with either control vehicle, or 1 × 106 neonatal-derived or 1 × 106 patient-derived iECs. Recovery in perfusion to the affected limb was measured using laser Doppler scanning. Perfusion recovery was enhanced in mice treated with neonatal-derived iECs and in two of the three patient-derived iEC lines investigated in vivo. Patient-derived iECs can be successfully generated from PAD patients and for specific patients display comparable pro-angiogenic properties to neonatal-derived iECs.
format	article
author	Jack D Hywood Sara Sadeghipour Zoe E Clayton Jun Yuan Colleen Stubbs Jack W T Wong John P Cooke Sanjay Patel
author_facet	Jack D Hywood Sara Sadeghipour Zoe E Clayton Jun Yuan Colleen Stubbs Jack W T Wong John P Cooke Sanjay Patel
author_sort	Jack D Hywood
title	Induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties.
title_short	Induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties.
title_full	Induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties.
title_fullStr	Induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties.
title_full_unstemmed	Induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties.
title_sort	induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0161145f479244b5965f069930afa8fb
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Induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties.

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