In vitro studies on oxidative stress-independent, Ag nanoparticles-induced cell toxicity of Candida albicans, an opportunistic pathogen

Venkatraman Srinivasan Radhakrishnan,1 Surya Prakash Dwivedi,2 Mohammed Haris Siddiqui,3 Tulika Prasad1 1Advanced Instrumentation Research Facility (AIRF), Jawaharlal Nehru University, New Delhi, 2School of Biotechnology, IFTM University, Moradabad, 3Department of Bioengineering, Integral Universit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Radhakrishnan VS, Dwivedi SP, Siddiqui MH, Prasad T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Silver Nanoparticles (AgNp) Candida albicans Multidrug resistance ergosterol Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0162e83f574b4bd6b67c971943c7bdb2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0162e83f574b4bd6b67c971943c7bdb2
record_format	dspace
spelling	oai:doaj.org-article:0162e83f574b4bd6b67c971943c7bdb22021-12-02T00:48:21ZIn vitro studies on oxidative stress-independent, Ag nanoparticles-induced cell toxicity of Candida albicans, an opportunistic pathogen1178-2013https://doaj.org/article/0162e83f574b4bd6b67c971943c7bdb22018-03-01T00:00:00Zhttps://www.dovepress.com/in-vitro-studies-on-oxidative-stress-independent-ag-nanoparticles-indu-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Venkatraman Srinivasan Radhakrishnan,1 Surya Prakash Dwivedi,2 Mohammed Haris Siddiqui,3 Tulika Prasad1 1Advanced Instrumentation Research Facility (AIRF), Jawaharlal Nehru University, New Delhi, 2School of Biotechnology, IFTM University, Moradabad, 3Department of Bioengineering, Integral University, Lucknow, Uttar Pradesh, India Abstract: Silver nanoparticles (AgNps) have attracted maximal attention among all metal nanoparticles, and the study of their biological properties has gained impetus for further medical adoption. This study evaluated the cellular and molecular mechanisms associated with the action of AgNps against an opportunistic pathogen, Candida albicans. Spherical, stable AgNp (average size 21.6 nm) prepared by a chemical reduction method showed minimum inhibitory concentration (required to inhibit the growth of 90% of organisms) at 40 µg/mL. AgNps have been reported to induce oxidative stress-mediated programmed cell death through the accumulation of intracellular reactive oxygen species (ROS). However, this study demonstrated that intracellular levels of AgNp-induced ROS could be reversed by using antioxidant ascorbic acid, but the sensitivity of AgNp-treated Candida cells could not be completely reversed. Moreover, in addition to the generation of ROS, the AgNps were found to affect other cellular targets resulting in altered membrane fluidity, membrane microenvironment, ergosterol content, cellular morphology, and ultrastructure. Thus, the generation of ROS does not seem to be the sole major cause of AgNp-mediated cell toxicity in Candida. Rather, the multitargeted action of AgNps, generation of ROS, alterations in ergosterol content, and membrane fluidity together seem to have potentiated anti-Candida action. Thus, this “nano-based drug therapy” is likely to favor broad-spectrum activity, multiple cellular targets, and minimum host toxicity. AgNps, therefore, appear to have the potential to address the challenges in multidrug resistance and fungal therapeutics. Keywords: antifungal, nanomedicine, ROS, drug resistanceRadhakrishnan VSDwivedi SPSiddiqui MHPrasad TDove Medical PressarticleSilver Nanoparticles (AgNp)Candida albicansMultidrug resistanceergosterolMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 91-96 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Silver Nanoparticles (AgNp) Candida albicans Multidrug resistance ergosterol Medicine (General) R5-920
spellingShingle	Silver Nanoparticles (AgNp) Candida albicans Multidrug resistance ergosterol Medicine (General) R5-920 Radhakrishnan VS Dwivedi SP Siddiqui MH Prasad T In vitro studies on oxidative stress-independent, Ag nanoparticles-induced cell toxicity of Candida albicans, an opportunistic pathogen
description	Venkatraman Srinivasan Radhakrishnan,1 Surya Prakash Dwivedi,2 Mohammed Haris Siddiqui,3 Tulika Prasad1 1Advanced Instrumentation Research Facility (AIRF), Jawaharlal Nehru University, New Delhi, 2School of Biotechnology, IFTM University, Moradabad, 3Department of Bioengineering, Integral University, Lucknow, Uttar Pradesh, India Abstract: Silver nanoparticles (AgNps) have attracted maximal attention among all metal nanoparticles, and the study of their biological properties has gained impetus for further medical adoption. This study evaluated the cellular and molecular mechanisms associated with the action of AgNps against an opportunistic pathogen, Candida albicans. Spherical, stable AgNp (average size 21.6 nm) prepared by a chemical reduction method showed minimum inhibitory concentration (required to inhibit the growth of 90% of organisms) at 40 µg/mL. AgNps have been reported to induce oxidative stress-mediated programmed cell death through the accumulation of intracellular reactive oxygen species (ROS). However, this study demonstrated that intracellular levels of AgNp-induced ROS could be reversed by using antioxidant ascorbic acid, but the sensitivity of AgNp-treated Candida cells could not be completely reversed. Moreover, in addition to the generation of ROS, the AgNps were found to affect other cellular targets resulting in altered membrane fluidity, membrane microenvironment, ergosterol content, cellular morphology, and ultrastructure. Thus, the generation of ROS does not seem to be the sole major cause of AgNp-mediated cell toxicity in Candida. Rather, the multitargeted action of AgNps, generation of ROS, alterations in ergosterol content, and membrane fluidity together seem to have potentiated anti-Candida action. Thus, this “nano-based drug therapy” is likely to favor broad-spectrum activity, multiple cellular targets, and minimum host toxicity. AgNps, therefore, appear to have the potential to address the challenges in multidrug resistance and fungal therapeutics. Keywords: antifungal, nanomedicine, ROS, drug resistance
format	article
author	Radhakrishnan VS Dwivedi SP Siddiqui MH Prasad T
author_facet	Radhakrishnan VS Dwivedi SP Siddiqui MH Prasad T
author_sort	Radhakrishnan VS
title	In vitro studies on oxidative stress-independent, Ag nanoparticles-induced cell toxicity of Candida albicans, an opportunistic pathogen
title_short	In vitro studies on oxidative stress-independent, Ag nanoparticles-induced cell toxicity of Candida albicans, an opportunistic pathogen
title_full	In vitro studies on oxidative stress-independent, Ag nanoparticles-induced cell toxicity of Candida albicans, an opportunistic pathogen
title_fullStr	In vitro studies on oxidative stress-independent, Ag nanoparticles-induced cell toxicity of Candida albicans, an opportunistic pathogen
title_full_unstemmed	In vitro studies on oxidative stress-independent, Ag nanoparticles-induced cell toxicity of Candida albicans, an opportunistic pathogen
title_sort	in vitro studies on oxidative stress-independent, ag nanoparticles-induced cell toxicity of candida albicans, an opportunistic pathogen
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/0162e83f574b4bd6b67c971943c7bdb2
work_keys_str_mv	AT radhakrishnanvs invitrostudiesonoxidativestressindependentagnanoparticlesinducedcelltoxicityofcandidaalbicansanopportunisticpathogen AT dwivedisp invitrostudiesonoxidativestressindependentagnanoparticlesinducedcelltoxicityofcandidaalbicansanopportunisticpathogen AT siddiquimh invitrostudiesonoxidativestressindependentagnanoparticlesinducedcelltoxicityofcandidaalbicansanopportunisticpathogen AT prasadt invitrostudiesonoxidativestressindependentagnanoparticlesinducedcelltoxicityofcandidaalbicansanopportunisticpathogen
_version_	1718403474891210752

In vitro studies on oxidative stress-independent, Ag nanoparticles-induced cell toxicity of Candida albicans, an opportunistic pathogen

Ejemplares similares