Genomic and Phylogenetic Analysis of Lactiplantibacillus plantarum L125, and Evaluation of Its Anti-Proliferative and Cytotoxic Activity in Cancer Cells

Lactiplantibacillus plantarum is a diverse species that includes nomadic strains isolated from a variety of environmental niches. Several L. plantarum strains are being incorporated in fermented foodstuffs as starter cultures, while some of them have also been c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Konstantinos Tegopoulos, Odysseas Sotirios Stergiou, Despoina Eugenia Kiousi, Margaritis Tsifintaris, Ellie Koletsou, Aristotelis C. Papageorgiou, Anthoula A. Argyri, Nikos Chorianopoulos, Alex Galanis, Petros Kolovos
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Lactiplantibacillus plantarum</i> genomics whole-genome sequencing probiotics comparative genomics phylogenetic analysis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/01644b18a4004e249e6b15d630efac2e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:01644b18a4004e249e6b15d630efac2e
record_format	dspace
spelling	oai:doaj.org-article:01644b18a4004e249e6b15d630efac2e2021-11-25T16:51:16ZGenomic and Phylogenetic Analysis of <i>Lactiplantibacillus plantarum</i> L125, and Evaluation of Its Anti-Proliferative and Cytotoxic Activity in Cancer Cells10.3390/biomedicines91117182227-9059https://doaj.org/article/01644b18a4004e249e6b15d630efac2e2021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1718https://doaj.org/toc/2227-9059<i>Lactiplantibacillus plantarum</i> is a diverse species that includes nomadic strains isolated from a variety of environmental niches. Several <i>L. plantarum</i> strains are being incorporated in fermented foodstuffs as starter cultures, while some of them have also been characterized as probiotics. In this study, we present the draft genome sequence of <i>L. plantarum</i> L125, a potential probiotic strain presenting biotechnological interest, originally isolated from a traditional fermented meat product. Phylogenetic and comparative genomic analysis with other potential probiotic <i>L. plantarum</i> strains were performed to determine its evolutionary relationships. Furthermore, we located genes involved in the probiotic phenotype by whole genome annotation. Indeed, genes coding for proteins mediating host–microbe interactions and bile salt, heat and cold stress tolerance were identified. Concerning the potential health-promoting attributes of the novel strain, we determined that <i>L. plantarum</i> L125 carries an incomplete plantaricin gene cluster, in agreement with previous in vitro findings, where no bacteriocin-like activity was detected. Moreover, we showed that cell-free culture supernatant (CFCS) of <i>L. plantarum</i> L125 exerts anti-proliferative, anti-clonogenic and anti-migration activity against the human colon adenocarcinoma cell line, HT-29. Conclusively, <i>L. plantarum</i> L125 presents desirable probiotic traits. Future studies will elucidate further its biological and health-related properties.Konstantinos TegopoulosOdysseas Sotirios StergiouDespoina Eugenia KiousiMargaritis TsifintarisEllie KoletsouAristotelis C. PapageorgiouAnthoula A. ArgyriNikos ChorianopoulosAlex GalanisPetros KolovosMDPI AGarticle<i>Lactiplantibacillus plantarum</i>genomicswhole-genome sequencingprobioticscomparative genomicsphylogenetic analysisBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1718, p 1718 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Lactiplantibacillus plantarum</i> genomics whole-genome sequencing probiotics comparative genomics phylogenetic analysis Biology (General) QH301-705.5
spellingShingle	<i>Lactiplantibacillus plantarum</i> genomics whole-genome sequencing probiotics comparative genomics phylogenetic analysis Biology (General) QH301-705.5 Konstantinos Tegopoulos Odysseas Sotirios Stergiou Despoina Eugenia Kiousi Margaritis Tsifintaris Ellie Koletsou Aristotelis C. Papageorgiou Anthoula A. Argyri Nikos Chorianopoulos Alex Galanis Petros Kolovos Genomic and Phylogenetic Analysis of <i>Lactiplantibacillus plantarum</i> L125, and Evaluation of Its Anti-Proliferative and Cytotoxic Activity in Cancer Cells
description	<i>Lactiplantibacillus plantarum</i> is a diverse species that includes nomadic strains isolated from a variety of environmental niches. Several <i>L. plantarum</i> strains are being incorporated in fermented foodstuffs as starter cultures, while some of them have also been characterized as probiotics. In this study, we present the draft genome sequence of <i>L. plantarum</i> L125, a potential probiotic strain presenting biotechnological interest, originally isolated from a traditional fermented meat product. Phylogenetic and comparative genomic analysis with other potential probiotic <i>L. plantarum</i> strains were performed to determine its evolutionary relationships. Furthermore, we located genes involved in the probiotic phenotype by whole genome annotation. Indeed, genes coding for proteins mediating host–microbe interactions and bile salt, heat and cold stress tolerance were identified. Concerning the potential health-promoting attributes of the novel strain, we determined that <i>L. plantarum</i> L125 carries an incomplete plantaricin gene cluster, in agreement with previous in vitro findings, where no bacteriocin-like activity was detected. Moreover, we showed that cell-free culture supernatant (CFCS) of <i>L. plantarum</i> L125 exerts anti-proliferative, anti-clonogenic and anti-migration activity against the human colon adenocarcinoma cell line, HT-29. Conclusively, <i>L. plantarum</i> L125 presents desirable probiotic traits. Future studies will elucidate further its biological and health-related properties.
format	article
author	Konstantinos Tegopoulos Odysseas Sotirios Stergiou Despoina Eugenia Kiousi Margaritis Tsifintaris Ellie Koletsou Aristotelis C. Papageorgiou Anthoula A. Argyri Nikos Chorianopoulos Alex Galanis Petros Kolovos
author_facet	Konstantinos Tegopoulos Odysseas Sotirios Stergiou Despoina Eugenia Kiousi Margaritis Tsifintaris Ellie Koletsou Aristotelis C. Papageorgiou Anthoula A. Argyri Nikos Chorianopoulos Alex Galanis Petros Kolovos
author_sort	Konstantinos Tegopoulos
title	Genomic and Phylogenetic Analysis of <i>Lactiplantibacillus plantarum</i> L125, and Evaluation of Its Anti-Proliferative and Cytotoxic Activity in Cancer Cells
title_short	Genomic and Phylogenetic Analysis of <i>Lactiplantibacillus plantarum</i> L125, and Evaluation of Its Anti-Proliferative and Cytotoxic Activity in Cancer Cells
title_full	Genomic and Phylogenetic Analysis of <i>Lactiplantibacillus plantarum</i> L125, and Evaluation of Its Anti-Proliferative and Cytotoxic Activity in Cancer Cells
title_fullStr	Genomic and Phylogenetic Analysis of <i>Lactiplantibacillus plantarum</i> L125, and Evaluation of Its Anti-Proliferative and Cytotoxic Activity in Cancer Cells
title_full_unstemmed	Genomic and Phylogenetic Analysis of <i>Lactiplantibacillus plantarum</i> L125, and Evaluation of Its Anti-Proliferative and Cytotoxic Activity in Cancer Cells
title_sort	genomic and phylogenetic analysis of <i>lactiplantibacillus plantarum</i> l125, and evaluation of its anti-proliferative and cytotoxic activity in cancer cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/01644b18a4004e249e6b15d630efac2e
work_keys_str_mv	AT konstantinostegopoulos genomicandphylogeneticanalysisofilactiplantibacillusplantarumil125andevaluationofitsantiproliferativeandcytotoxicactivityincancercells AT odysseassotiriosstergiou genomicandphylogeneticanalysisofilactiplantibacillusplantarumil125andevaluationofitsantiproliferativeandcytotoxicactivityincancercells AT despoinaeugeniakiousi genomicandphylogeneticanalysisofilactiplantibacillusplantarumil125andevaluationofitsantiproliferativeandcytotoxicactivityincancercells AT margaritistsifintaris genomicandphylogeneticanalysisofilactiplantibacillusplantarumil125andevaluationofitsantiproliferativeandcytotoxicactivityincancercells AT elliekoletsou genomicandphylogeneticanalysisofilactiplantibacillusplantarumil125andevaluationofitsantiproliferativeandcytotoxicactivityincancercells AT aristoteliscpapageorgiou genomicandphylogeneticanalysisofilactiplantibacillusplantarumil125andevaluationofitsantiproliferativeandcytotoxicactivityincancercells AT anthoulaaargyri genomicandphylogeneticanalysisofilactiplantibacillusplantarumil125andevaluationofitsantiproliferativeandcytotoxicactivityincancercells AT nikoschorianopoulos genomicandphylogeneticanalysisofilactiplantibacillusplantarumil125andevaluationofitsantiproliferativeandcytotoxicactivityincancercells AT alexgalanis genomicandphylogeneticanalysisofilactiplantibacillusplantarumil125andevaluationofitsantiproliferativeandcytotoxicactivityincancercells AT petroskolovos genomicandphylogeneticanalysisofilactiplantibacillusplantarumil125andevaluationofitsantiproliferativeandcytotoxicactivityincancercells
_version_	1718412875372953600

Genomic and Phylogenetic Analysis of <i>Lactiplantibacillus plantarum</i> L125, and Evaluation of Its Anti-Proliferative and Cytotoxic Activity in Cancer Cells

Ejemplares similares