A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.

Centrosomes comprise a pair of centrioles surrounded by an amorphous pericentriolar material (PCM). Here, we have performed a microscopy-based genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins required for centriole duplication and mitotic PCM recruitment. We analys...

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Autores principales:	Jeroen Dobbelaere, Filipe Josué, Saskia Suijkerbuijk, Buzz Baum, Nicolas Tapon, Jordan Raff
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0164a7f2fa144f4e9a1595fc7254c021
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spelling	oai:doaj.org-article:0164a7f2fa144f4e9a1595fc7254c0212021-11-25T05:33:57ZA genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.1544-91731545-788510.1371/journal.pbio.0060224https://doaj.org/article/0164a7f2fa144f4e9a1595fc7254c0212008-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18798690/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Centrosomes comprise a pair of centrioles surrounded by an amorphous pericentriolar material (PCM). Here, we have performed a microscopy-based genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins required for centriole duplication and mitotic PCM recruitment. We analysed 92% of the Drosophila genome (13,059 genes) and identified 32 genes involved in centrosome function. An extensive series of secondary screens classified these genes into four categories: (1) nine are required for centriole duplication, (2) 11 are required for centrosome maturation, (3) nine are required for both functions, and (4) three genes regulate centrosome separation. These 32 hits include several new centrosomal components, some of which have human homologs. In addition, we find that the individual depletion of only two proteins, Polo and Centrosomin (Cnn) can completely block centrosome maturation. Cnn is phosphorylated during mitosis in a Polo-dependent manner, suggesting that the Polo-dependent phosphorylation of Cnn initiates centrosome maturation in flies.Jeroen DobbelaereFilipe JosuéSaskia SuijkerbuijkBuzz BaumNicolas TaponJordan RaffPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 6, Iss 9, p e224 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Jeroen Dobbelaere Filipe Josué Saskia Suijkerbuijk Buzz Baum Nicolas Tapon Jordan Raff A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.
description	Centrosomes comprise a pair of centrioles surrounded by an amorphous pericentriolar material (PCM). Here, we have performed a microscopy-based genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins required for centriole duplication and mitotic PCM recruitment. We analysed 92% of the Drosophila genome (13,059 genes) and identified 32 genes involved in centrosome function. An extensive series of secondary screens classified these genes into four categories: (1) nine are required for centriole duplication, (2) 11 are required for centrosome maturation, (3) nine are required for both functions, and (4) three genes regulate centrosome separation. These 32 hits include several new centrosomal components, some of which have human homologs. In addition, we find that the individual depletion of only two proteins, Polo and Centrosomin (Cnn) can completely block centrosome maturation. Cnn is phosphorylated during mitosis in a Polo-dependent manner, suggesting that the Polo-dependent phosphorylation of Cnn initiates centrosome maturation in flies.
format	article
author	Jeroen Dobbelaere Filipe Josué Saskia Suijkerbuijk Buzz Baum Nicolas Tapon Jordan Raff
author_facet	Jeroen Dobbelaere Filipe Josué Saskia Suijkerbuijk Buzz Baum Nicolas Tapon Jordan Raff
author_sort	Jeroen Dobbelaere
title	A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.
title_short	A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.
title_full	A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.
title_fullStr	A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.
title_full_unstemmed	A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.
title_sort	genome-wide rnai screen to dissect centriole duplication and centrosome maturation in drosophila.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/0164a7f2fa144f4e9a1595fc7254c021
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A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila.

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