AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines

The utility of AsCas12a can be limited to poor editing efficiency. Here the authors identify a variant, “AsCas12a Ultra”, that has high on-target specificity demonstrated through editing of clinically relevant T cell genes.

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Bibliographic Details
Main Authors:	Liyang Zhang, John A. Zuris, Ramya Viswanathan, Jasmine N. Edelstein, Rolf Turk, Bernice Thommandru, H. Tomas Rube, Steve E. Glenn, Michael A. Collingwood, Nicole M. Bode, Sarah F. Beaudoin, Swarali Lele, Sean N. Scott, Kevin M. Wasko, Steven Sexton, Christopher M. Borges, Mollie S. Schubert, Gavin L. Kurgan, Matthew S. McNeill, Cecilia A. Fernandez, Vic E. Myer, Richard A. Morgan, Mark A. Behlke, Christopher A. Vakulskas
Format:	article
Language:	EN
Published:	Nature Portfolio 2021
Subjects:	Science Q
Online Access:	https://doaj.org/article/0168163683a24415a8a5f32e5a19e09a
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spelling	oai:doaj.org-article:0168163683a24415a8a5f32e5a19e09a2021-12-02T18:02:47ZAsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines10.1038/s41467-021-24017-82041-1723https://doaj.org/article/0168163683a24415a8a5f32e5a19e09a2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24017-8https://doaj.org/toc/2041-1723The utility of AsCas12a can be limited to poor editing efficiency. Here the authors identify a variant, “AsCas12a Ultra”, that has high on-target specificity demonstrated through editing of clinically relevant T cell genes.Liyang ZhangJohn A. ZurisRamya ViswanathanJasmine N. EdelsteinRolf TurkBernice ThommandruH. Tomas RubeSteve E. GlennMichael A. CollingwoodNicole M. BodeSarah F. BeaudoinSwarali LeleSean N. ScottKevin M. WaskoSteven SextonChristopher M. BorgesMollie S. SchubertGavin L. KurganMatthew S. McNeillCecilia A. FernandezVic E. MyerRichard A. MorganMark A. BehlkeChristopher A. VakulskasNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Liyang Zhang John A. Zuris Ramya Viswanathan Jasmine N. Edelstein Rolf Turk Bernice Thommandru H. Tomas Rube Steve E. Glenn Michael A. Collingwood Nicole M. Bode Sarah F. Beaudoin Swarali Lele Sean N. Scott Kevin M. Wasko Steven Sexton Christopher M. Borges Mollie S. Schubert Gavin L. Kurgan Matthew S. McNeill Cecilia A. Fernandez Vic E. Myer Richard A. Morgan Mark A. Behlke Christopher A. Vakulskas AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines
description	The utility of AsCas12a can be limited to poor editing efficiency. Here the authors identify a variant, “AsCas12a Ultra”, that has high on-target specificity demonstrated through editing of clinically relevant T cell genes.
format	article
author	Liyang Zhang John A. Zuris Ramya Viswanathan Jasmine N. Edelstein Rolf Turk Bernice Thommandru H. Tomas Rube Steve E. Glenn Michael A. Collingwood Nicole M. Bode Sarah F. Beaudoin Swarali Lele Sean N. Scott Kevin M. Wasko Steven Sexton Christopher M. Borges Mollie S. Schubert Gavin L. Kurgan Matthew S. McNeill Cecilia A. Fernandez Vic E. Myer Richard A. Morgan Mark A. Behlke Christopher A. Vakulskas
author_facet	Liyang Zhang John A. Zuris Ramya Viswanathan Jasmine N. Edelstein Rolf Turk Bernice Thommandru H. Tomas Rube Steve E. Glenn Michael A. Collingwood Nicole M. Bode Sarah F. Beaudoin Swarali Lele Sean N. Scott Kevin M. Wasko Steven Sexton Christopher M. Borges Mollie S. Schubert Gavin L. Kurgan Matthew S. McNeill Cecilia A. Fernandez Vic E. Myer Richard A. Morgan Mark A. Behlke Christopher A. Vakulskas
author_sort	Liyang Zhang
title	AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines
title_short	AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines
title_full	AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines
title_fullStr	AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines
title_full_unstemmed	AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines
title_sort	ascas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0168163683a24415a8a5f32e5a19e09a
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AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines

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