Phycocyanin/PEG-b-(PG-g-PEI) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality

Fei Tong,1 Xiangyuan Tang,2 Daojun Liu2 1Department of Pathology and Pathophysiology, Provincial Key Discipline of Pharmacology, Jiaxing University Medical College, Jiaxing, Zhejiang Province, PR China; 2Department of Pharmaceutical Chemistry, Medical College, Shantou University, Shantou, PR China...

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Autores principales:	Tong F, Tang X, Liu D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	phycocyanin PEG-b-(PG-g-PEI) HI/RIPII pancreatic islets Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/016bc040ae1d4539be361bb0b569315a
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spelling	oai:doaj.org-article:016bc040ae1d4539be361bb0b569315a2021-12-02T05:19:57ZPhycocyanin/PEG-b-(PG-g-PEI) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality1178-2013https://doaj.org/article/016bc040ae1d4539be361bb0b569315a2019-01-01T00:00:00Zhttps://www.dovepress.com/phycocyaninpeg-b-pg-g-pei-attenuated-hepatic-ischemiareperfusion-induc-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fei Tong,1 Xiangyuan Tang,2 Daojun Liu2 1Department of Pathology and Pathophysiology, Provincial Key Discipline of Pharmacology, Jiaxing University Medical College, Jiaxing, Zhejiang Province, PR China; 2Department of Pharmaceutical Chemistry, Medical College, Shantou University, Shantou, PR China Background: Hepatic ischemia/reperfusion-induced pancreatic islet injury (HI/RIPII) was an important pathophysiological phenomenon in clinics. In the present study, we observed the effects of phycocyanin on HI/RIPII. However, the half-life of phycocyanin was extremely short and limited its use in vivo. Materials and methods: In order to overcome this shortcoming, poly(ethylene glycol)-b-(poly(L-glutamic acid)-g-polyethylenimine) (PEG-b-(PG-g-PEI)) was synthesized and estimated as a nanocarrier for lengthening delivery of phycocyanin through the abdominal subcutaneous injection in rats. Phycocyanin (isoelectric point=4.3) was encapsulated with PEG-b-(PG-g-PEI) via electrostatic interactions at pH 7.4. Results: In vitro phycocyanin was fast and efficiently encapsulated and showing efficient loading and sustained release. In vivo the anti-HI/RIPII function of phycocyanin/PEG-b-(PG-g-PEI) complex was surveyed in rats using free phycocyanin as the controls, and the results showed that phycocyanin/PEG-b-(PG-g-PEI) complex reduced HI/RIPII property and enlarged islet functionality. Conclusion: These results suggested that PEG-b-(PG-g-PEI) might be treated as a potential phycocyanin nanocarrier. Keywords: phycocyanin, PEG-b-(PG-g-PEI), HI/RIPII, pancreatic isletsTong FTang XLiu DDove Medical PressarticlephycocyaninPEG-b-(PG-g-PEI)HI/RIPIIpancreatic isletsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 339-351 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	phycocyanin PEG-b-(PG-g-PEI) HI/RIPII pancreatic islets Medicine (General) R5-920
spellingShingle	phycocyanin PEG-b-(PG-g-PEI) HI/RIPII pancreatic islets Medicine (General) R5-920 Tong F Tang X Liu D Phycocyanin/PEG-b-(PG-g-PEI) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality
description	Fei Tong,1 Xiangyuan Tang,2 Daojun Liu2 1Department of Pathology and Pathophysiology, Provincial Key Discipline of Pharmacology, Jiaxing University Medical College, Jiaxing, Zhejiang Province, PR China; 2Department of Pharmaceutical Chemistry, Medical College, Shantou University, Shantou, PR China Background: Hepatic ischemia/reperfusion-induced pancreatic islet injury (HI/RIPII) was an important pathophysiological phenomenon in clinics. In the present study, we observed the effects of phycocyanin on HI/RIPII. However, the half-life of phycocyanin was extremely short and limited its use in vivo. Materials and methods: In order to overcome this shortcoming, poly(ethylene glycol)-b-(poly(L-glutamic acid)-g-polyethylenimine) (PEG-b-(PG-g-PEI)) was synthesized and estimated as a nanocarrier for lengthening delivery of phycocyanin through the abdominal subcutaneous injection in rats. Phycocyanin (isoelectric point=4.3) was encapsulated with PEG-b-(PG-g-PEI) via electrostatic interactions at pH 7.4. Results: In vitro phycocyanin was fast and efficiently encapsulated and showing efficient loading and sustained release. In vivo the anti-HI/RIPII function of phycocyanin/PEG-b-(PG-g-PEI) complex was surveyed in rats using free phycocyanin as the controls, and the results showed that phycocyanin/PEG-b-(PG-g-PEI) complex reduced HI/RIPII property and enlarged islet functionality. Conclusion: These results suggested that PEG-b-(PG-g-PEI) might be treated as a potential phycocyanin nanocarrier. Keywords: phycocyanin, PEG-b-(PG-g-PEI), HI/RIPII, pancreatic islets
format	article
author	Tong F Tang X Liu D
author_facet	Tong F Tang X Liu D
author_sort	Tong F
title	Phycocyanin/PEG-b-(PG-g-PEI) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality
title_short	Phycocyanin/PEG-b-(PG-g-PEI) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality
title_full	Phycocyanin/PEG-b-(PG-g-PEI) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality
title_fullStr	Phycocyanin/PEG-b-(PG-g-PEI) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality
title_full_unstemmed	Phycocyanin/PEG-b-(PG-g-PEI) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality
title_sort	phycocyanin/peg-b-(pg-g-pei) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/016bc040ae1d4539be361bb0b569315a
work_keys_str_mv	AT tongf phycocyaninpegbpggpeiattenuatedhepaticischemiareperfusioninducedpancreaticisletinjuryandenlargedisletfunctionality AT tangx phycocyaninpegbpggpeiattenuatedhepaticischemiareperfusioninducedpancreaticisletinjuryandenlargedisletfunctionality AT liud phycocyaninpegbpggpeiattenuatedhepaticischemiareperfusioninducedpancreaticisletinjuryandenlargedisletfunctionality
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Phycocyanin/PEG-b-(PG-g-PEI) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality

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