The histone replacement gene His4r is involved in heat stress induced chromatin rearrangement

Abstract His4r is the only known variant of histone H4 in Drosophila. It is encoded by the His4r single-copy gene that is located outside of the histone gene cluster and expressed in a different pattern than H4, although the encoded polypeptides are identical. We generated a null mutant (His4r Δ42 )...

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Autores principales:	Anikó Faragó, Adél Ürmösi, Anita Farkas, László Bodai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/016c556be0be46c59193be82f96ab16f
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spelling	oai:doaj.org-article:016c556be0be46c59193be82f96ab16f2021-12-02T13:34:32ZThe histone replacement gene His4r is involved in heat stress induced chromatin rearrangement10.1038/s41598-021-84413-42045-2322https://doaj.org/article/016c556be0be46c59193be82f96ab16f2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84413-4https://doaj.org/toc/2045-2322Abstract His4r is the only known variant of histone H4 in Drosophila. It is encoded by the His4r single-copy gene that is located outside of the histone gene cluster and expressed in a different pattern than H4, although the encoded polypeptides are identical. We generated a null mutant (His4r Δ42 ) which is homozygous viable and fertile without any apparent morphological defects. Heterozygous His4r Δ42 is a mild suppressor of position-effect variegation, suggesting that His4r has a role in the formation or maintenance of condensed chromatin. Under standard conditions loss of His4r has a modest effect on gene expression. Upon heat-stress the induction of the Heat shock protein (HSP) genes Hsp27 and Hsp68 is stronger in His4r Δ42 mutants with concordantly increased survival rate. Analysis of chromatin accessibility after heat shock at a Hsp27 regulatory region showed less condensed chromatin in the absence of His4r while there was no difference at the gene body. Interestingly, preconditioning before heat shock led to increased chromatin accessibility, HSP gene transcription and survival rate in control flies while it did not cause notable changes in His4r Δ42 . Thus, our results suggest that His4r might play a role in fine tuning chromatin structure at inducible gene promoters upon environmental stress conditions.Anikó FaragóAdél ÜrmösiAnita FarkasLászló BodaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anikó Faragó Adél Ürmösi Anita Farkas László Bodai The histone replacement gene His4r is involved in heat stress induced chromatin rearrangement
description	Abstract His4r is the only known variant of histone H4 in Drosophila. It is encoded by the His4r single-copy gene that is located outside of the histone gene cluster and expressed in a different pattern than H4, although the encoded polypeptides are identical. We generated a null mutant (His4r Δ42 ) which is homozygous viable and fertile without any apparent morphological defects. Heterozygous His4r Δ42 is a mild suppressor of position-effect variegation, suggesting that His4r has a role in the formation or maintenance of condensed chromatin. Under standard conditions loss of His4r has a modest effect on gene expression. Upon heat-stress the induction of the Heat shock protein (HSP) genes Hsp27 and Hsp68 is stronger in His4r Δ42 mutants with concordantly increased survival rate. Analysis of chromatin accessibility after heat shock at a Hsp27 regulatory region showed less condensed chromatin in the absence of His4r while there was no difference at the gene body. Interestingly, preconditioning before heat shock led to increased chromatin accessibility, HSP gene transcription and survival rate in control flies while it did not cause notable changes in His4r Δ42 . Thus, our results suggest that His4r might play a role in fine tuning chromatin structure at inducible gene promoters upon environmental stress conditions.
format	article
author	Anikó Faragó Adél Ürmösi Anita Farkas László Bodai
author_facet	Anikó Faragó Adél Ürmösi Anita Farkas László Bodai
author_sort	Anikó Faragó
title	The histone replacement gene His4r is involved in heat stress induced chromatin rearrangement
title_short	The histone replacement gene His4r is involved in heat stress induced chromatin rearrangement
title_full	The histone replacement gene His4r is involved in heat stress induced chromatin rearrangement
title_fullStr	The histone replacement gene His4r is involved in heat stress induced chromatin rearrangement
title_full_unstemmed	The histone replacement gene His4r is involved in heat stress induced chromatin rearrangement
title_sort	histone replacement gene his4r is involved in heat stress induced chromatin rearrangement
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/016c556be0be46c59193be82f96ab16f
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The histone replacement gene His4r is involved in heat stress induced chromatin rearrangement

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