Safety assessment of β-fructofuranosidase from

β-Fructofuranosidase (β-D-fructofuranoside fructohydrolase; EC 3.2.1.26) is used in the production of fructo-oligosaccharides that are commonly used by the food industry as prebiotics for their purported health benefits. The β-fructofuranosidase discussed herein is obtained from a novel source organ...

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Autores principales:	Trung Duc Vo, Jwar Meetro, Barry Lynch, Shahrzad Tafazoli, Akio Ichihara, Go Chikamatsu
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Toxicology. Poisons RA1190-1270
Acceso en línea:	https://doaj.org/article/0170d028a41545b181de2d1e41595cc2
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spelling	oai:doaj.org-article:0170d028a41545b181de2d1e41595cc22021-11-15T04:03:22ZSafety assessment of β-fructofuranosidase from 2397-847310.1177/23978473211055361https://doaj.org/article/0170d028a41545b181de2d1e41595cc22021-11-01T00:00:00Zhttps://doi.org/10.1177/23978473211055361https://doaj.org/toc/2397-8473β-Fructofuranosidase (β-D-fructofuranoside fructohydrolase; EC 3.2.1.26) is used in the production of fructo-oligosaccharides that are commonly used by the food industry as prebiotics for their purported health benefits. The β-fructofuranosidase discussed herein is obtained from a novel source organism that is a non-genetically modified strain of Aspergillus brunneoviolaceus , which phylogenetically belongs to the Aspergillus section Nigri. The safety of β-fructofuranosidase was evaluated in a series of toxicology studies as prescribed by Tier 1 toxicity testing by the European Food Safety Authority, including an evaluation of the mutagenicity and genotoxicity potential using the in vitro bacterial reverse mutation and mammalian chromosomal aberration assays, as well as systemic toxicity in a 90-day oral subchronic toxicity study in Sprague-Dawley rats. β-Fructofuranosidase was demonstrated to lack mutagenic or genotoxic potential based on the results of the in vitro assays due to absence of increased revertant colonies in the bacterial reverse mutation test and incidence of chromosome aberrations in the chromosomal aberration assay. Administration of β-fructofuranosidase by gavage at doses up to 1200 mg total organic solids (TOS)/kg body weight/day for 90 days did not elicit any systemic toxic effects in rats based on a lack of adverse effect in any study parameter, and therefore the no-observed-adverse-effect level of β-fructofuranosidase was concluded to be 1200 mg TOS/kg body weight/day, the highest dose tested. The results of the toxicology studies on β-fructofuranosidase from A. brunneoviolaceus demonstrate this species to be a safe and suitable source of enzymes for use by the food industry.Trung Duc VoJwar MeetroBarry LynchShahrzad TafazoliAkio IchiharaGo ChikamatsuSAGE PublishingarticleToxicology. PoisonsRA1190-1270ENToxicology Research and Application, Vol 5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Toxicology. Poisons RA1190-1270
spellingShingle	Toxicology. Poisons RA1190-1270 Trung Duc Vo Jwar Meetro Barry Lynch Shahrzad Tafazoli Akio Ichihara Go Chikamatsu Safety assessment of β-fructofuranosidase from
description	β-Fructofuranosidase (β-D-fructofuranoside fructohydrolase; EC 3.2.1.26) is used in the production of fructo-oligosaccharides that are commonly used by the food industry as prebiotics for their purported health benefits. The β-fructofuranosidase discussed herein is obtained from a novel source organism that is a non-genetically modified strain of Aspergillus brunneoviolaceus , which phylogenetically belongs to the Aspergillus section Nigri. The safety of β-fructofuranosidase was evaluated in a series of toxicology studies as prescribed by Tier 1 toxicity testing by the European Food Safety Authority, including an evaluation of the mutagenicity and genotoxicity potential using the in vitro bacterial reverse mutation and mammalian chromosomal aberration assays, as well as systemic toxicity in a 90-day oral subchronic toxicity study in Sprague-Dawley rats. β-Fructofuranosidase was demonstrated to lack mutagenic or genotoxic potential based on the results of the in vitro assays due to absence of increased revertant colonies in the bacterial reverse mutation test and incidence of chromosome aberrations in the chromosomal aberration assay. Administration of β-fructofuranosidase by gavage at doses up to 1200 mg total organic solids (TOS)/kg body weight/day for 90 days did not elicit any systemic toxic effects in rats based on a lack of adverse effect in any study parameter, and therefore the no-observed-adverse-effect level of β-fructofuranosidase was concluded to be 1200 mg TOS/kg body weight/day, the highest dose tested. The results of the toxicology studies on β-fructofuranosidase from A. brunneoviolaceus demonstrate this species to be a safe and suitable source of enzymes for use by the food industry.
format	article
author	Trung Duc Vo Jwar Meetro Barry Lynch Shahrzad Tafazoli Akio Ichihara Go Chikamatsu
author_facet	Trung Duc Vo Jwar Meetro Barry Lynch Shahrzad Tafazoli Akio Ichihara Go Chikamatsu
author_sort	Trung Duc Vo
title	Safety assessment of β-fructofuranosidase from
title_short	Safety assessment of β-fructofuranosidase from
title_full	Safety assessment of β-fructofuranosidase from
title_fullStr	Safety assessment of β-fructofuranosidase from
title_full_unstemmed	Safety assessment of β-fructofuranosidase from
title_sort	safety assessment of β-fructofuranosidase from
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/0170d028a41545b181de2d1e41595cc2
work_keys_str_mv	AT trungducvo safetyassessmentofbfructofuranosidasefrom AT jwarmeetro safetyassessmentofbfructofuranosidasefrom AT barrylynch safetyassessmentofbfructofuranosidasefrom AT shahrzadtafazoli safetyassessmentofbfructofuranosidasefrom AT akioichihara safetyassessmentofbfructofuranosidasefrom AT gochikamatsu safetyassessmentofbfructofuranosidasefrom
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Safety assessment of β-fructofuranosidase from

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