In vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages

Jian Li,1 Sia Lee Yoong,2 Wei Jiang Goh,2 Bertrand Czarny,1 Zhi Yang,1 Kingshuk Poddar,2,3 Michal M Dykas,2,3 Abhijeet Patra,2,3 T Venkatesan,2,3 Tomasz Panczyk,4 Chengkuo Lee,5 Giorgia Pastorin1–3 1Department of Pharmacy, National University of Singapore, 2NUS Graduate School for Integra...

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Autores principales:	Li J, Yoong SL, Goh WJ, Czarny B, Yang Z, Poddar K, Dykas MM, Patra A, Venkatesan T, Panczyk T, Lee C, Pastorin G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	carbon nanotubes surface functionalization cleavable bonds cisplatin drug delivery Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0172a7898815413e97bca65bcc282eda
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spelling	oai:doaj.org-article:0172a7898815413e97bca65bcc282eda2021-12-02T03:58:33ZIn vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages1178-2013https://doaj.org/article/0172a7898815413e97bca65bcc282eda2015-12-01T00:00:00Zhttps://www.dovepress.com/in-vitro-controlled-release-of-cisplatin-from-gold-carbon-nanobottles--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jian Li,1 Sia Lee Yoong,2 Wei Jiang Goh,2 Bertrand Czarny,1 Zhi Yang,1 Kingshuk Poddar,2,3 Michal M Dykas,2,3 Abhijeet Patra,2,3 T Venkatesan,2,3 Tomasz Panczyk,4 Chengkuo Lee,5 Giorgia Pastorin1–3 1Department of Pharmacy, National University of Singapore, 2NUS Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences (CeLS), 3NUSNNI-NanoCore, National University of Singapore, Singapore; 4Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Cracow, Poland; 5Department of Electrical and Computer Engineering, National University of Singapore, Singapore Abstract: Carbon nanotubes’ (CNTs) hollow interior space has been explored for biomedical applications, such as drug repository against undesirable inactivation. To further devise CNTs as smart material for controlled release of cargo molecules, we propose the concept of “gold-carbon nanobottles”. After encapsulating cis-diammineplatinum(II) dichloride (cisplatin, CDDP) in CNTs, we covalently attached gold nanoparticles (AuNPs) at the open-tips of CNTs via different cleavable linkages, namely hydrazine, ester, and disulfide-containing linkages. Compared with our previous study in which more than 80% of CDDP leaked from CNTs in 2 hours, AuNPs were found to significantly decrease such spontaneous release to <40%. In addition, CDDP release from AuNP-capped CNTs via disulfide linkage was selectively enhanced by twofolds in reducing conditions (namely with 1 mM dithiothreitol [DTT]), which mimic the intracellular environment. We treated human colon adenocarcinoma cells HCT116 with our CDDP-loaded gold-carbon nanobottles and examined the cell viability using lactate dehydrogenase assay. Interestingly, we found that our nanobottles with cleavable disulfide linkage exerted stronger cytotoxic effect in HCT116 compared with normal human fetal lung fibroblast cells IMR-90. Therefore, we infer that our nanobottles strategy with inbuilt disulfide linkage could attain selective release of payload in highly reductive tumor tissues while avoiding collateral damage to normal tissues. Keywords: carbon nanotubes, surface functionalization, cleavable bonds, cisplatin, drug deliveryLi JYoong SLGoh WJCzarny BYang ZPoddar KDykas MMPatra AVenkatesan TPanczyk TLee CPastorin GDove Medical Pressarticlecarbon nanotubessurface functionalizationcleavable bondscisplatindrug deliveryMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 7425-7441 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbon nanotubes surface functionalization cleavable bonds cisplatin drug delivery Medicine (General) R5-920
spellingShingle	carbon nanotubes surface functionalization cleavable bonds cisplatin drug delivery Medicine (General) R5-920 Li J Yoong SL Goh WJ Czarny B Yang Z Poddar K Dykas MM Patra A Venkatesan T Panczyk T Lee C Pastorin G In vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages
description	Jian Li,1 Sia Lee Yoong,2 Wei Jiang Goh,2 Bertrand Czarny,1 Zhi Yang,1 Kingshuk Poddar,2,3 Michal M Dykas,2,3 Abhijeet Patra,2,3 T Venkatesan,2,3 Tomasz Panczyk,4 Chengkuo Lee,5 Giorgia Pastorin1–3 1Department of Pharmacy, National University of Singapore, 2NUS Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences (CeLS), 3NUSNNI-NanoCore, National University of Singapore, Singapore; 4Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Cracow, Poland; 5Department of Electrical and Computer Engineering, National University of Singapore, Singapore Abstract: Carbon nanotubes’ (CNTs) hollow interior space has been explored for biomedical applications, such as drug repository against undesirable inactivation. To further devise CNTs as smart material for controlled release of cargo molecules, we propose the concept of “gold-carbon nanobottles”. After encapsulating cis-diammineplatinum(II) dichloride (cisplatin, CDDP) in CNTs, we covalently attached gold nanoparticles (AuNPs) at the open-tips of CNTs via different cleavable linkages, namely hydrazine, ester, and disulfide-containing linkages. Compared with our previous study in which more than 80% of CDDP leaked from CNTs in 2 hours, AuNPs were found to significantly decrease such spontaneous release to <40%. In addition, CDDP release from AuNP-capped CNTs via disulfide linkage was selectively enhanced by twofolds in reducing conditions (namely with 1 mM dithiothreitol [DTT]), which mimic the intracellular environment. We treated human colon adenocarcinoma cells HCT116 with our CDDP-loaded gold-carbon nanobottles and examined the cell viability using lactate dehydrogenase assay. Interestingly, we found that our nanobottles with cleavable disulfide linkage exerted stronger cytotoxic effect in HCT116 compared with normal human fetal lung fibroblast cells IMR-90. Therefore, we infer that our nanobottles strategy with inbuilt disulfide linkage could attain selective release of payload in highly reductive tumor tissues while avoiding collateral damage to normal tissues. Keywords: carbon nanotubes, surface functionalization, cleavable bonds, cisplatin, drug delivery
format	article
author	Li J Yoong SL Goh WJ Czarny B Yang Z Poddar K Dykas MM Patra A Venkatesan T Panczyk T Lee C Pastorin G
author_facet	Li J Yoong SL Goh WJ Czarny B Yang Z Poddar K Dykas MM Patra A Venkatesan T Panczyk T Lee C Pastorin G
author_sort	Li J
title	In vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages
title_short	In vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages
title_full	In vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages
title_fullStr	In vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages
title_full_unstemmed	In vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages
title_sort	in vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/0172a7898815413e97bca65bcc282eda
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In vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages

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