Cholic acid-modified polyethylenimine: in vitro and in vivo studies

Brahmanand Dube,1,2 Abhijeet Pandey,1 Ganesh Joshi,3 Rita Mulherkar,3 Krutika Sawant1 1Pharmacy Department, Faculty of Pharmacy, The Maharaja Sayajirao University of Baroda, Vadodara, 2Wockhardt Research Centre, Wockhardt Ltd, Aurangabad, India; 3Genetic Engineering Laboratory, ACTREC Tata Memorial...

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Autores principales:	Dube B, Pandey A, Joshi G, Mulherkar R, Sawant K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Polyethylenimine biodistribution tumor regression. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/017a3eba2b4440fb80e6b1a57bb7e914
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spelling	oai:doaj.org-article:017a3eba2b4440fb80e6b1a57bb7e9142021-12-02T07:45:56ZCholic acid-modified polyethylenimine: in vitro and in vivo studies1178-2013https://doaj.org/article/017a3eba2b4440fb80e6b1a57bb7e9142018-03-01T00:00:00Zhttps://www.dovepress.com/cholic-acid-modified-polyethylenimine-in-vitro-and-in-vivo-studies-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Brahmanand Dube,1,2 Abhijeet Pandey,1 Ganesh Joshi,3 Rita Mulherkar,3 Krutika Sawant1 1Pharmacy Department, Faculty of Pharmacy, The Maharaja Sayajirao University of Baroda, Vadodara, 2Wockhardt Research Centre, Wockhardt Ltd, Aurangabad, India; 3Genetic Engineering Laboratory, ACTREC Tata Memorial Centre, Kharghar, Navi Mumbai Abstract: Low-molecular-weight polyethylenimine has lower cytotoxicity than high molecular weight polyethylenimine, but it is not an efficient transfection agent because of limitations of DNA delivery into the cytoplasm. Therefore, in the present study, the hydrophobic modification of low-molecular-weight polyethylenimine (PEI 2 kDa [PEI2]) by cholic acid (ChA) was performed to form PEI2-ChA, and in vitro and in vivo studies were performed. Results indicate that the nanoplexes of PEI2-ChA with gWIZ-GFP have greater transfection efficiency (27%) in NT8e cell lines as evaluated by flow cytometry and also observed by fluorescence imaging. The present study concluded that the transferrin-containing nanoplexes of PEI2-ChA conjugates with plasmid p53 warrant clinical trials in humans after exhaustive animal studies for use as a novel gene delivery system. Keywords: polyethylenimine, biodistribution, tumor regressionDube BPandey AJoshi GMulherkar RSawant KDove Medical PressarticlePolyethyleniminebiodistributiontumor regression.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 83-85 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Polyethylenimine biodistribution tumor regression. Medicine (General) R5-920
spellingShingle	Polyethylenimine biodistribution tumor regression. Medicine (General) R5-920 Dube B Pandey A Joshi G Mulherkar R Sawant K Cholic acid-modified polyethylenimine: in vitro and in vivo studies
description	Brahmanand Dube,1,2 Abhijeet Pandey,1 Ganesh Joshi,3 Rita Mulherkar,3 Krutika Sawant1 1Pharmacy Department, Faculty of Pharmacy, The Maharaja Sayajirao University of Baroda, Vadodara, 2Wockhardt Research Centre, Wockhardt Ltd, Aurangabad, India; 3Genetic Engineering Laboratory, ACTREC Tata Memorial Centre, Kharghar, Navi Mumbai Abstract: Low-molecular-weight polyethylenimine has lower cytotoxicity than high molecular weight polyethylenimine, but it is not an efficient transfection agent because of limitations of DNA delivery into the cytoplasm. Therefore, in the present study, the hydrophobic modification of low-molecular-weight polyethylenimine (PEI 2 kDa [PEI2]) by cholic acid (ChA) was performed to form PEI2-ChA, and in vitro and in vivo studies were performed. Results indicate that the nanoplexes of PEI2-ChA with gWIZ-GFP have greater transfection efficiency (27%) in NT8e cell lines as evaluated by flow cytometry and also observed by fluorescence imaging. The present study concluded that the transferrin-containing nanoplexes of PEI2-ChA conjugates with plasmid p53 warrant clinical trials in humans after exhaustive animal studies for use as a novel gene delivery system. Keywords: polyethylenimine, biodistribution, tumor regression
format	article
author	Dube B Pandey A Joshi G Mulherkar R Sawant K
author_facet	Dube B Pandey A Joshi G Mulherkar R Sawant K
author_sort	Dube B
title	Cholic acid-modified polyethylenimine: in vitro and in vivo studies
title_short	Cholic acid-modified polyethylenimine: in vitro and in vivo studies
title_full	Cholic acid-modified polyethylenimine: in vitro and in vivo studies
title_fullStr	Cholic acid-modified polyethylenimine: in vitro and in vivo studies
title_full_unstemmed	Cholic acid-modified polyethylenimine: in vitro and in vivo studies
title_sort	cholic acid-modified polyethylenimine: in vitro and in vivo studies
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/017a3eba2b4440fb80e6b1a57bb7e914
work_keys_str_mv	AT dubeb cholicacidmodifiedpolyethylenimineinvitroandinvivostudies AT pandeya cholicacidmodifiedpolyethylenimineinvitroandinvivostudies AT joshig cholicacidmodifiedpolyethylenimineinvitroandinvivostudies AT mulherkarr cholicacidmodifiedpolyethylenimineinvitroandinvivostudies AT sawantk cholicacidmodifiedpolyethylenimineinvitroandinvivostudies
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Cholic acid-modified polyethylenimine: in vitro and in vivo studies

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