Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids

Abstract. Sukma D, Handini AS, Sudarsono. 2020. Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids. Biodiversitas 21: 5054-5064. Chalcone synthase (CHS) is a key enzyme in flavonoid biosynthesis. The research aims to isolate and characterize th...

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Autores principales:	Dewi Sukma, Aline Sisi Handini, Sudarsono Sudarsono
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	chs gene, dna sequencing, genetic diversity analysis, multiple sequence alignment, phylogenetic analysis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/01885fd4c6ce495c93312cc4ab3fc108
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spelling	oai:doaj.org-article:01885fd4c6ce495c93312cc4ab3fc1082021-11-22T00:44:11ZIsolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids1412-033X2085-472210.13057/biodiv/d211109https://doaj.org/article/01885fd4c6ce495c93312cc4ab3fc1082020-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6689https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Sukma D, Handini AS, Sudarsono. 2020. Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids. Biodiversitas 21: 5054-5064. Chalcone synthase (CHS) is a key enzyme in flavonoid biosynthesis. The research aims to isolate and characterize the CHS gene nucleotide sequence diversity of nine Phalaenopsis and one Doritaenopsis genotypes. Genomic DNA was isolated from young leaves and used to PCR amplify the gene using CHS specific degenerate primers. Results of PCR amplification yielded DNA fragments of 700 bp. Upon sequencing and nucleotide sequence analysis, we found that the genomic fragments were partial CHS gene from Phalaenopsis and Doritaenopsis genotypes and deposited the sequences in the NCBI Genebank Database accession numbers of KR184089-KR184098. More analysis of the sequences confirmed they shared ranged from 81-99% sequence identities to known CHS genes from Phalaenopsis deposited in the NCBI Database. They also shared 84-100% amino acid residues identity to CHS polypeptides. Multiple sequence alignment (MSA) and phylogenetic analysis further revealed the determined CHS nucleotide sequences from Phalaenopsis and Doritaenopsis genotypes were closely related to CHS genes from other orchid species.Dewi SukmaAline Sisi HandiniSudarsono SudarsonoMBI & UNS Soloarticlechs gene, dna sequencing, genetic diversity analysis, multiple sequence alignment, phylogenetic analysisBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	chs gene, dna sequencing, genetic diversity analysis, multiple sequence alignment, phylogenetic analysis Biology (General) QH301-705.5
spellingShingle	chs gene, dna sequencing, genetic diversity analysis, multiple sequence alignment, phylogenetic analysis Biology (General) QH301-705.5 Dewi Sukma Aline Sisi Handini Sudarsono Sudarsono Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids
description	Abstract. Sukma D, Handini AS, Sudarsono. 2020. Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids. Biodiversitas 21: 5054-5064. Chalcone synthase (CHS) is a key enzyme in flavonoid biosynthesis. The research aims to isolate and characterize the CHS gene nucleotide sequence diversity of nine Phalaenopsis and one Doritaenopsis genotypes. Genomic DNA was isolated from young leaves and used to PCR amplify the gene using CHS specific degenerate primers. Results of PCR amplification yielded DNA fragments of 700 bp. Upon sequencing and nucleotide sequence analysis, we found that the genomic fragments were partial CHS gene from Phalaenopsis and Doritaenopsis genotypes and deposited the sequences in the NCBI Genebank Database accession numbers of KR184089-KR184098. More analysis of the sequences confirmed they shared ranged from 81-99% sequence identities to known CHS genes from Phalaenopsis deposited in the NCBI Database. They also shared 84-100% amino acid residues identity to CHS polypeptides. Multiple sequence alignment (MSA) and phylogenetic analysis further revealed the determined CHS nucleotide sequences from Phalaenopsis and Doritaenopsis genotypes were closely related to CHS genes from other orchid species.
format	article
author	Dewi Sukma Aline Sisi Handini Sudarsono Sudarsono
author_facet	Dewi Sukma Aline Sisi Handini Sudarsono Sudarsono
author_sort	Dewi Sukma
title	Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids
title_short	Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids
title_full	Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids
title_fullStr	Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids
title_full_unstemmed	Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids
title_sort	isolation and characterization of chalcone synthase (chs) gene from phalaenopsis and doritaenopsis orchids
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/01885fd4c6ce495c93312cc4ab3fc108
work_keys_str_mv	AT dewisukma isolationandcharacterizationofchalconesynthasechsgenefromphalaenopsisanddoritaenopsisorchids AT alinesisihandini isolationandcharacterizationofchalconesynthasechsgenefromphalaenopsisanddoritaenopsisorchids AT sudarsonosudarsono isolationandcharacterizationofchalconesynthasechsgenefromphalaenopsisanddoritaenopsisorchids
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Isolation and characterization of chalcone synthase (CHS) gene from Phalaenopsis and Doritaenopsis orchids

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