Inflammatory gene mRNA expression in human peripheral blood and its association with colorectal cancer

Reem Abdullah Alamro,1 Mohammad Mustafa,2 Abdulrahman K Al-Asmari2 1Deparment of Gastroenterology, Prince Sultan Military Medical City, Riyadh, Saudi Arabia; 2Research Centre, Prince Sultan Military Medical City, Riyadh, Saudi Arabia Objective: The present study planned to investigate the changes in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alamro RA, Mustafa M, Al-Asmari AK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	colorectal cancer mRNA expression COX-2 TNF-α NF-kB IL-6 Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/0191de01655c4550b52aa22006e6b664
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0191de01655c4550b52aa22006e6b664
record_format	dspace
spelling	oai:doaj.org-article:0191de01655c4550b52aa22006e6b6642021-12-02T05:36:41ZInflammatory gene mRNA expression in human peripheral blood and its association with colorectal cancer1178-7031https://doaj.org/article/0191de01655c4550b52aa22006e6b6642018-09-01T00:00:00Zhttps://www.dovepress.com/inflammatory-gene-mrna-expression-in-human-peripheral-blood-and-its-as-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Reem Abdullah Alamro,1 Mohammad Mustafa,2 Abdulrahman K Al-Asmari2 1Deparment of Gastroenterology, Prince Sultan Military Medical City, Riyadh, Saudi Arabia; 2Research Centre, Prince Sultan Military Medical City, Riyadh, Saudi Arabia Objective: The present study planned to investigate the changes in the mRNA expression of inflammatory genes and their association with colorectal cancer (CRC). Our findings could be useful for noninvasive early screening of CRC patients. Patients and methods: Venous blood of 20 CRC cases and 15 healthy controls was collected. The mRNA expression of COX-2, TNF-α, NF-κB and IL-6 genes was carried out by using real-time polymerase chain reaction. Relative quantification was done to find out the fold change of these genes. Results: The mean age of cases and controls was 55 and 50 years, respectively. The ΔCt of COX-2, TNF-α, NF-κB and IL-6 genes was significantly (p < 0.05) lower in cases as compared to controls. Subsequently, the mRNA expression of these genes was, respectively, 3.56-, 3.4-, 1.71- and 3.86-fold higher in CRC cases as compared to controls. Positive correlation of ΔCt of COX-2 was found with ΔCt of TNF-α (r = 0.461, p = 0.041) and NF-κB (r = 0.536, p = 0.015) in CRC cases. The mRNA expression of COX-2 was significantly lower in T2 stage, while mRNA expression of NF-κB was significantly lower in both T2 and T3 stages of CRC as compared to T4 stage. Conclusion: The increased mRNA expression of COX-2 along with the high mRNA expression of TNF-α, NF-κB and IL-6 genes may be associative risk factors for CRC. COX-2 and NF-κB genes were more expressed in advanced stages of CRC indicating their role in tumor progression. Our findings support the possible role of blood biomarker in the screening of CRC patients in the early stages. Keywords: colorectal cancer, mRNA expression, COX-2, TNF-α, NF-κB, IL-6, blood biomarkerAlamro RAMustafa MAl-Asmari AKDove Medical Pressarticlecolorectal cancermRNA expressionCOX-2TNF-αNF-kBIL-6PathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 11, Pp 351-357 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	colorectal cancer mRNA expression COX-2 TNF-α NF-kB IL-6 Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	colorectal cancer mRNA expression COX-2 TNF-α NF-kB IL-6 Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Alamro RA Mustafa M Al-Asmari AK Inflammatory gene mRNA expression in human peripheral blood and its association with colorectal cancer
description	Reem Abdullah Alamro,1 Mohammad Mustafa,2 Abdulrahman K Al-Asmari2 1Deparment of Gastroenterology, Prince Sultan Military Medical City, Riyadh, Saudi Arabia; 2Research Centre, Prince Sultan Military Medical City, Riyadh, Saudi Arabia Objective: The present study planned to investigate the changes in the mRNA expression of inflammatory genes and their association with colorectal cancer (CRC). Our findings could be useful for noninvasive early screening of CRC patients. Patients and methods: Venous blood of 20 CRC cases and 15 healthy controls was collected. The mRNA expression of COX-2, TNF-α, NF-κB and IL-6 genes was carried out by using real-time polymerase chain reaction. Relative quantification was done to find out the fold change of these genes. Results: The mean age of cases and controls was 55 and 50 years, respectively. The ΔCt of COX-2, TNF-α, NF-κB and IL-6 genes was significantly (p < 0.05) lower in cases as compared to controls. Subsequently, the mRNA expression of these genes was, respectively, 3.56-, 3.4-, 1.71- and 3.86-fold higher in CRC cases as compared to controls. Positive correlation of ΔCt of COX-2 was found with ΔCt of TNF-α (r = 0.461, p = 0.041) and NF-κB (r = 0.536, p = 0.015) in CRC cases. The mRNA expression of COX-2 was significantly lower in T2 stage, while mRNA expression of NF-κB was significantly lower in both T2 and T3 stages of CRC as compared to T4 stage. Conclusion: The increased mRNA expression of COX-2 along with the high mRNA expression of TNF-α, NF-κB and IL-6 genes may be associative risk factors for CRC. COX-2 and NF-κB genes were more expressed in advanced stages of CRC indicating their role in tumor progression. Our findings support the possible role of blood biomarker in the screening of CRC patients in the early stages. Keywords: colorectal cancer, mRNA expression, COX-2, TNF-α, NF-κB, IL-6, blood biomarker
format	article
author	Alamro RA Mustafa M Al-Asmari AK
author_facet	Alamro RA Mustafa M Al-Asmari AK
author_sort	Alamro RA
title	Inflammatory gene mRNA expression in human peripheral blood and its association with colorectal cancer
title_short	Inflammatory gene mRNA expression in human peripheral blood and its association with colorectal cancer
title_full	Inflammatory gene mRNA expression in human peripheral blood and its association with colorectal cancer
title_fullStr	Inflammatory gene mRNA expression in human peripheral blood and its association with colorectal cancer
title_full_unstemmed	Inflammatory gene mRNA expression in human peripheral blood and its association with colorectal cancer
title_sort	inflammatory gene mrna expression in human peripheral blood and its association with colorectal cancer
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/0191de01655c4550b52aa22006e6b664
work_keys_str_mv	AT alamrora inflammatorygenemrnaexpressioninhumanperipheralbloodanditsassociationwithcolorectalcancer AT mustafam inflammatorygenemrnaexpressioninhumanperipheralbloodanditsassociationwithcolorectalcancer AT alasmariak inflammatorygenemrnaexpressioninhumanperipheralbloodanditsassociationwithcolorectalcancer
_version_	1718400272470900736

Inflammatory gene mRNA expression in human peripheral blood and its association with colorectal cancer

Ejemplares similares