An electrochemical sensor for high sensitive determination of lysozyme based on the aptamer competition approach

Protein is a kind of basic substance that constitutes a life body. The determination of protein is very important for the research of biology, medicine, and other fields. Lysozyme is relatively small and simple in structure among all kinds of proteins, so it is often used as a standard target detect...

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Autores principales:	Song Kai, Chen Wenwu
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	differential pulse voltammetry lysozyme daunomycin aptamer multi-walled carbon nanotubes electrochemical analysis Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/019a673157464e3ba2f9c9c7f57e80f1
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spelling	oai:doaj.org-article:019a673157464e3ba2f9c9c7f57e80f12021-12-05T14:10:43ZAn electrochemical sensor for high sensitive determination of lysozyme based on the aptamer competition approach2391-542010.1515/chem-2021-0026https://doaj.org/article/019a673157464e3ba2f9c9c7f57e80f12021-03-01T00:00:00Zhttps://doi.org/10.1515/chem-2021-0026https://doaj.org/toc/2391-5420Protein is a kind of basic substance that constitutes a life body. The determination of protein is very important for the research of biology, medicine, and other fields. Lysozyme is relatively small and simple in structure among all kinds of proteins, so it is often used as a standard target detector in the study of aptamer sensor for protein detection. In this paper, a lysozyme electrochemical sensor based on aptamer competition mechanism is proposed. We have successfully prepared a signal weakening electrochemical sensor based on the lysozyme aptamer competition mechanism. The carboxylated multi-walled carbon nanotubes (MWCNTs) were modified on the glassy carbon electrode, and the complementary aptamer DNA with amino group was connected to MWCNTs. Because of the complementary DNA of daunomycin into the electrode, the electrochemical signal is generated. When there is a target, the aptamer binds to lysozyme with higher binding power, and the original complementary chain breaks down, resulting in the loss of daunomycin inserted into the double chain and the weakening of electrochemical signal. Differential pulse voltammetry was used to determine lysozyme, the response range was 1–500 nM, the correlation coefficient was 0.9995, and the detection limit was 0.5 nM. In addition, the proposed sensor has good selectivity and anti-interference.Song KaiChen WenwuDe Gruyterarticledifferential pulse voltammetrylysozymedaunomycinaptamermulti-walled carbon nanotubeselectrochemical analysisChemistryQD1-999ENOpen Chemistry, Vol 19, Iss 1, Pp 299-306 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	differential pulse voltammetry lysozyme daunomycin aptamer multi-walled carbon nanotubes electrochemical analysis Chemistry QD1-999
spellingShingle	differential pulse voltammetry lysozyme daunomycin aptamer multi-walled carbon nanotubes electrochemical analysis Chemistry QD1-999 Song Kai Chen Wenwu An electrochemical sensor for high sensitive determination of lysozyme based on the aptamer competition approach
description	Protein is a kind of basic substance that constitutes a life body. The determination of protein is very important for the research of biology, medicine, and other fields. Lysozyme is relatively small and simple in structure among all kinds of proteins, so it is often used as a standard target detector in the study of aptamer sensor for protein detection. In this paper, a lysozyme electrochemical sensor based on aptamer competition mechanism is proposed. We have successfully prepared a signal weakening electrochemical sensor based on the lysozyme aptamer competition mechanism. The carboxylated multi-walled carbon nanotubes (MWCNTs) were modified on the glassy carbon electrode, and the complementary aptamer DNA with amino group was connected to MWCNTs. Because of the complementary DNA of daunomycin into the electrode, the electrochemical signal is generated. When there is a target, the aptamer binds to lysozyme with higher binding power, and the original complementary chain breaks down, resulting in the loss of daunomycin inserted into the double chain and the weakening of electrochemical signal. Differential pulse voltammetry was used to determine lysozyme, the response range was 1–500 nM, the correlation coefficient was 0.9995, and the detection limit was 0.5 nM. In addition, the proposed sensor has good selectivity and anti-interference.
format	article
author	Song Kai Chen Wenwu
author_facet	Song Kai Chen Wenwu
author_sort	Song Kai
title	An electrochemical sensor for high sensitive determination of lysozyme based on the aptamer competition approach
title_short	An electrochemical sensor for high sensitive determination of lysozyme based on the aptamer competition approach
title_full	An electrochemical sensor for high sensitive determination of lysozyme based on the aptamer competition approach
title_fullStr	An electrochemical sensor for high sensitive determination of lysozyme based on the aptamer competition approach
title_full_unstemmed	An electrochemical sensor for high sensitive determination of lysozyme based on the aptamer competition approach
title_sort	electrochemical sensor for high sensitive determination of lysozyme based on the aptamer competition approach
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/019a673157464e3ba2f9c9c7f57e80f1
work_keys_str_mv	AT songkai anelectrochemicalsensorforhighsensitivedeterminationoflysozymebasedontheaptamercompetitionapproach AT chenwenwu anelectrochemicalsensorforhighsensitivedeterminationoflysozymebasedontheaptamercompetitionapproach AT songkai electrochemicalsensorforhighsensitivedeterminationoflysozymebasedontheaptamercompetitionapproach AT chenwenwu electrochemicalsensorforhighsensitivedeterminationoflysozymebasedontheaptamercompetitionapproach
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An electrochemical sensor for high sensitive determination of lysozyme based on the aptamer competition approach

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