The Waddlia genome: a window into chlamydial biology.

Growing evidence suggests that a novel member of the Chlamydiales order, Waddlia chondrophila, is a potential agent of miscarriage in humans and abortion in ruminants. Due to the lack of genetic tools to manipulate chlamydia, genomic analysis is proving to be the most incisive tool in stimulating in...

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Autores principales:	Claire Bertelli, François Collyn, Antony Croxatto, Christian Rückert, Adam Polkinghorne, Carole Kebbi-Beghdadi, Alexander Goesmann, Lloyd Vaughan, Gilbert Greub
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/019f8c504c5f46c4adca676627acf333
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spelling	oai:doaj.org-article:019f8c504c5f46c4adca676627acf3332021-12-02T20:21:16ZThe Waddlia genome: a window into chlamydial biology.1932-620310.1371/journal.pone.0010890https://doaj.org/article/019f8c504c5f46c4adca676627acf3332010-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20531937/?tool=EBIhttps://doaj.org/toc/1932-6203Growing evidence suggests that a novel member of the Chlamydiales order, Waddlia chondrophila, is a potential agent of miscarriage in humans and abortion in ruminants. Due to the lack of genetic tools to manipulate chlamydia, genomic analysis is proving to be the most incisive tool in stimulating investigations into the biology of these obligate intracellular bacteria. 454/Roche and Solexa/Illumina technologies were thus used to sequence and assemble de novo the full genome of the first representative of the Waddliaceae family, W. chondrophila. The bacteria possesses a 2'116'312 bp chromosome and a 15'593 bp low-copy number plasmid that might integrate into the bacterial chromosome. The Waddlia genome displays numerous repeated sequences indicating different genome dynamics from classical chlamydia which almost completely lack repetitive elements. Moreover, W. chondrophila exhibits many virulence factors also present in classical chlamydia, including a functional type III secretion system, but also a large complement of specific factors for resistance to host or environmental stresses. Large families of outer membrane proteins were identified indicating that these highly immunogenic proteins are not Chlamydiaceae specific and might have been present in their last common ancestor. Enhanced metabolic capability for the synthesis of nucleotides, amino acids, lipids and other co-factors suggests that the common ancestor of the modern Chlamydiales may have been less dependent on their eukaryotic host. The fine-detailed analysis of biosynthetic pathways brings us closer to possibly developing a synthetic medium to grow W. chondrophila, a critical step in the development of genetic tools. As a whole, the availability of the W. chondrophila genome opens new possibilities in Chlamydiales research, providing new insights into the evolution of members of the order Chlamydiales and the biology of the Waddliaceae.Claire BertelliFrançois CollynAntony CroxattoChristian RückertAdam PolkinghorneAdam PolkinghorneCarole Kebbi-BeghdadiAlexander GoesmannLloyd VaughanGilbert GreubPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 5, p e10890 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claire Bertelli François Collyn Antony Croxatto Christian Rückert Adam Polkinghorne Adam Polkinghorne Carole Kebbi-Beghdadi Alexander Goesmann Lloyd Vaughan Gilbert Greub The Waddlia genome: a window into chlamydial biology.
description	Growing evidence suggests that a novel member of the Chlamydiales order, Waddlia chondrophila, is a potential agent of miscarriage in humans and abortion in ruminants. Due to the lack of genetic tools to manipulate chlamydia, genomic analysis is proving to be the most incisive tool in stimulating investigations into the biology of these obligate intracellular bacteria. 454/Roche and Solexa/Illumina technologies were thus used to sequence and assemble de novo the full genome of the first representative of the Waddliaceae family, W. chondrophila. The bacteria possesses a 2'116'312 bp chromosome and a 15'593 bp low-copy number plasmid that might integrate into the bacterial chromosome. The Waddlia genome displays numerous repeated sequences indicating different genome dynamics from classical chlamydia which almost completely lack repetitive elements. Moreover, W. chondrophila exhibits many virulence factors also present in classical chlamydia, including a functional type III secretion system, but also a large complement of specific factors for resistance to host or environmental stresses. Large families of outer membrane proteins were identified indicating that these highly immunogenic proteins are not Chlamydiaceae specific and might have been present in their last common ancestor. Enhanced metabolic capability for the synthesis of nucleotides, amino acids, lipids and other co-factors suggests that the common ancestor of the modern Chlamydiales may have been less dependent on their eukaryotic host. The fine-detailed analysis of biosynthetic pathways brings us closer to possibly developing a synthetic medium to grow W. chondrophila, a critical step in the development of genetic tools. As a whole, the availability of the W. chondrophila genome opens new possibilities in Chlamydiales research, providing new insights into the evolution of members of the order Chlamydiales and the biology of the Waddliaceae.
format	article
author	Claire Bertelli François Collyn Antony Croxatto Christian Rückert Adam Polkinghorne Adam Polkinghorne Carole Kebbi-Beghdadi Alexander Goesmann Lloyd Vaughan Gilbert Greub
author_facet	Claire Bertelli François Collyn Antony Croxatto Christian Rückert Adam Polkinghorne Adam Polkinghorne Carole Kebbi-Beghdadi Alexander Goesmann Lloyd Vaughan Gilbert Greub
author_sort	Claire Bertelli
title	The Waddlia genome: a window into chlamydial biology.
title_short	The Waddlia genome: a window into chlamydial biology.
title_full	The Waddlia genome: a window into chlamydial biology.
title_fullStr	The Waddlia genome: a window into chlamydial biology.
title_full_unstemmed	The Waddlia genome: a window into chlamydial biology.
title_sort	waddlia genome: a window into chlamydial biology.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/019f8c504c5f46c4adca676627acf333
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The Waddlia genome: a window into chlamydial biology.

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