Hyperexpression of TLR2 and TLR4 in patients with ischemic stroke in acute period of the disease

Pathogenesis of ischemic stroke is actively involved in the system of innate immunity. Under conditions of cerebral ischemia, a number of biologically active substances are released that interact with innate immunity receptors, in particular TLR2 and TLR4, which exacerbate inflammation...

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Autores principales:	L. V. Gankovskaya, L. V. Stakhovskaya, V. V. Grechenko, E. A. Koltsova, O. S. Uvarova, M. D. Demina, T. V. Gromova, O. A. Svitich
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2020
Materias:	ischemic stroke toll-like receptors tlr2 tlr4 innate immunity inflammation Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/01b082caf02c4e63b3a408965470e8c7
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spelling	oai:doaj.org-article:01b082caf02c4e63b3a408965470e8c72021-11-18T08:03:49ZHyperexpression of TLR2 and TLR4 in patients with ischemic stroke in acute period of the disease1563-06252313-741X10.15789/1563-0625-HOT-1971https://doaj.org/article/01b082caf02c4e63b3a408965470e8c72020-08-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1971https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XPathogenesis of ischemic stroke is actively involved in the system of innate immunity. Under conditions of cerebral ischemia, a number of biologically active substances are released that interact with innate immunity receptors, in particular TLR2 and TLR4, which exacerbate inflammation in brain tissue. Identification of predictor markers at the level of the innate immunity system may foresee the clinical course of ischemic stroke and ensure timely treatment. Our objective was to study expression of TLR2 and TLR4 receptors in peripheral blood leukocytes in patients with ischemic stroke in the dynamics of the disease. 27 people were included in the study. The main group consisted of patients with ischemic stroke of varying severity (n = 19). Patients of the main group were divided into two subgroups: with an NIHSS index value of < 10 (n = 10) and > 10 (n = 9). The control group included healthy donors with no history of acute and chronic inflammatory diseases (n = 8). Peripheral blood leukocytes were used as the test material. To determine expression of the TLR2 and TLR4 genes, RT-PCR in real time was used. Surface expression of TLRs was determined by flow cytometry. A study of the TLR2 and TLR4 gene expression showed that on the 1st, 3rd and 7th day post-stroke, the TLR4 gene expression in patients was significantly increased, when compared to the control group (p < 0.01), whereas TLR2 gene expression on the 3rd day of the disease was not statistically different from the control group. A study of surface expression of receptors showed that the average TLR2 fluorescence intensity on the patients’ peripheral blood monocytes was significantly increased on the 1st and 3rd day of disease when compared to the control group. The surface expression of TLR4 on monocytes has a statistically significant increase only on day 7. Assessment of surface expression of TLRs in subgroups with different severity values by NIHSS showed that patients with a NIHSS index > 10 had a significantly higher level of surface of TLR2 expression over the observation period, while the largest difference in TLR4 expression in the subgroups was observed on the 1st day of the disease (p < 0.05). Patients with ischemic stroke showed an increase in TLR2 and TLR4 expression at the gene and protein level, compared to healthy donors. These indices can be considered possible predictors for clinical prognosis of ischemic stroke.L. V. GankovskayaL. V. StakhovskayaV. V. GrechenkoE. A. KoltsovaO. S. UvarovaM. D. DeminaT. V. GromovaO. A. SvitichSPb RAACIarticleischemic stroketoll-like receptorstlr2tlr4innate immunityinflammationImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 22, Iss 4, Pp 665-674 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	ischemic stroke toll-like receptors tlr2 tlr4 innate immunity inflammation Immunologic diseases. Allergy RC581-607
spellingShingle	ischemic stroke toll-like receptors tlr2 tlr4 innate immunity inflammation Immunologic diseases. Allergy RC581-607 L. V. Gankovskaya L. V. Stakhovskaya V. V. Grechenko E. A. Koltsova O. S. Uvarova M. D. Demina T. V. Gromova O. A. Svitich Hyperexpression of TLR2 and TLR4 in patients with ischemic stroke in acute period of the disease
description	Pathogenesis of ischemic stroke is actively involved in the system of innate immunity. Under conditions of cerebral ischemia, a number of biologically active substances are released that interact with innate immunity receptors, in particular TLR2 and TLR4, which exacerbate inflammation in brain tissue. Identification of predictor markers at the level of the innate immunity system may foresee the clinical course of ischemic stroke and ensure timely treatment. Our objective was to study expression of TLR2 and TLR4 receptors in peripheral blood leukocytes in patients with ischemic stroke in the dynamics of the disease. 27 people were included in the study. The main group consisted of patients with ischemic stroke of varying severity (n = 19). Patients of the main group were divided into two subgroups: with an NIHSS index value of < 10 (n = 10) and > 10 (n = 9). The control group included healthy donors with no history of acute and chronic inflammatory diseases (n = 8). Peripheral blood leukocytes were used as the test material. To determine expression of the TLR2 and TLR4 genes, RT-PCR in real time was used. Surface expression of TLRs was determined by flow cytometry. A study of the TLR2 and TLR4 gene expression showed that on the 1st, 3rd and 7th day post-stroke, the TLR4 gene expression in patients was significantly increased, when compared to the control group (p < 0.01), whereas TLR2 gene expression on the 3rd day of the disease was not statistically different from the control group. A study of surface expression of receptors showed that the average TLR2 fluorescence intensity on the patients’ peripheral blood monocytes was significantly increased on the 1st and 3rd day of disease when compared to the control group. The surface expression of TLR4 on monocytes has a statistically significant increase only on day 7. Assessment of surface expression of TLRs in subgroups with different severity values by NIHSS showed that patients with a NIHSS index > 10 had a significantly higher level of surface of TLR2 expression over the observation period, while the largest difference in TLR4 expression in the subgroups was observed on the 1st day of the disease (p < 0.05). Patients with ischemic stroke showed an increase in TLR2 and TLR4 expression at the gene and protein level, compared to healthy donors. These indices can be considered possible predictors for clinical prognosis of ischemic stroke.
format	article
author	L. V. Gankovskaya L. V. Stakhovskaya V. V. Grechenko E. A. Koltsova O. S. Uvarova M. D. Demina T. V. Gromova O. A. Svitich
author_facet	L. V. Gankovskaya L. V. Stakhovskaya V. V. Grechenko E. A. Koltsova O. S. Uvarova M. D. Demina T. V. Gromova O. A. Svitich
author_sort	L. V. Gankovskaya
title	Hyperexpression of TLR2 and TLR4 in patients with ischemic stroke in acute period of the disease
title_short	Hyperexpression of TLR2 and TLR4 in patients with ischemic stroke in acute period of the disease
title_full	Hyperexpression of TLR2 and TLR4 in patients with ischemic stroke in acute period of the disease
title_fullStr	Hyperexpression of TLR2 and TLR4 in patients with ischemic stroke in acute period of the disease
title_full_unstemmed	Hyperexpression of TLR2 and TLR4 in patients with ischemic stroke in acute period of the disease
title_sort	hyperexpression of tlr2 and tlr4 in patients with ischemic stroke in acute period of the disease
publisher	SPb RAACI
publishDate	2020
url	https://doaj.org/article/01b082caf02c4e63b3a408965470e8c7
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Hyperexpression of TLR2 and TLR4 in patients with ischemic stroke in acute period of the disease

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