Barcode ITS2: a useful tool for identifying Trachelospermum jasminoides and a good monitor for medicine market

Abstract Trachelospermum jasminoides is commonly used in traditional Chinese medicine. However, the use of the plant’s local alternatives is frequent, causing potential clinical problems. The T. jasminoides sold in the medicine market is commonly dried and sliced, making traditional identification m...

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Autores principales:	Ning Yu, Yu-long Wei, Xin Zhang, Ning Zhu, Yan-li Wang, Yue Zhu, Hai-ping Zhang, Fen-mei Li, Lan Yang, Jia-qi Sun, Ai-dong Sun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/01b4dbdc7a9a49b5bac4ddba012d82f7
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spelling	oai:doaj.org-article:01b4dbdc7a9a49b5bac4ddba012d82f72021-12-02T11:53:12ZBarcode ITS2: a useful tool for identifying Trachelospermum jasminoides and a good monitor for medicine market10.1038/s41598-017-04674-w2045-2322https://doaj.org/article/01b4dbdc7a9a49b5bac4ddba012d82f72017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04674-whttps://doaj.org/toc/2045-2322Abstract Trachelospermum jasminoides is commonly used in traditional Chinese medicine. However, the use of the plant’s local alternatives is frequent, causing potential clinical problems. The T. jasminoides sold in the medicine market is commonly dried and sliced, making traditional identification methods difficult. In this study, the ITS2 region was evaluated on 127 sequences representing T. jasminoides and its local alternatives according to PCR and sequencing rates, intra- and inter-specific divergences, secondary structure, and discrimination capacity. Results indicated the 100% success rates of PCR and sequencing and the obvious presence of a barcoding gap. Results of BLAST 1, nearest distance and neighbor-joining tree methods showed that barcode ITS2 could successfully identify all the texted samples. The secondary structures of the ITS2 region provided another dimensionality for species identification. Two-dimensional images were obtained for better and easier identification. Previous studies on DNA barcoding concentrated more on the same family, genus, or species. However, an ideal barcode should be variable enough to identify closely related species. Meanwhile, the barcodes should also be conservative in identifying distantly related species. This study highlights the application of barcode ITS2 in solving practical problems in the distantly related local alternatives of medical plants.Ning YuYu-long WeiXin ZhangNing ZhuYan-li WangYue ZhuHai-ping ZhangFen-mei LiLan YangJia-qi SunAi-dong SunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ning Yu Yu-long Wei Xin Zhang Ning Zhu Yan-li Wang Yue Zhu Hai-ping Zhang Fen-mei Li Lan Yang Jia-qi Sun Ai-dong Sun Barcode ITS2: a useful tool for identifying Trachelospermum jasminoides and a good monitor for medicine market
description	Abstract Trachelospermum jasminoides is commonly used in traditional Chinese medicine. However, the use of the plant’s local alternatives is frequent, causing potential clinical problems. The T. jasminoides sold in the medicine market is commonly dried and sliced, making traditional identification methods difficult. In this study, the ITS2 region was evaluated on 127 sequences representing T. jasminoides and its local alternatives according to PCR and sequencing rates, intra- and inter-specific divergences, secondary structure, and discrimination capacity. Results indicated the 100% success rates of PCR and sequencing and the obvious presence of a barcoding gap. Results of BLAST 1, nearest distance and neighbor-joining tree methods showed that barcode ITS2 could successfully identify all the texted samples. The secondary structures of the ITS2 region provided another dimensionality for species identification. Two-dimensional images were obtained for better and easier identification. Previous studies on DNA barcoding concentrated more on the same family, genus, or species. However, an ideal barcode should be variable enough to identify closely related species. Meanwhile, the barcodes should also be conservative in identifying distantly related species. This study highlights the application of barcode ITS2 in solving practical problems in the distantly related local alternatives of medical plants.
format	article
author	Ning Yu Yu-long Wei Xin Zhang Ning Zhu Yan-li Wang Yue Zhu Hai-ping Zhang Fen-mei Li Lan Yang Jia-qi Sun Ai-dong Sun
author_facet	Ning Yu Yu-long Wei Xin Zhang Ning Zhu Yan-li Wang Yue Zhu Hai-ping Zhang Fen-mei Li Lan Yang Jia-qi Sun Ai-dong Sun
author_sort	Ning Yu
title	Barcode ITS2: a useful tool for identifying Trachelospermum jasminoides and a good monitor for medicine market
title_short	Barcode ITS2: a useful tool for identifying Trachelospermum jasminoides and a good monitor for medicine market
title_full	Barcode ITS2: a useful tool for identifying Trachelospermum jasminoides and a good monitor for medicine market
title_fullStr	Barcode ITS2: a useful tool for identifying Trachelospermum jasminoides and a good monitor for medicine market
title_full_unstemmed	Barcode ITS2: a useful tool for identifying Trachelospermum jasminoides and a good monitor for medicine market
title_sort	barcode its2: a useful tool for identifying trachelospermum jasminoides and a good monitor for medicine market
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/01b4dbdc7a9a49b5bac4ddba012d82f7
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Barcode ITS2: a useful tool for identifying Trachelospermum jasminoides and a good monitor for medicine market

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