Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1

Azo dyes are widely used in textile industries. A significant portion of these recalcitrant dyes are being discharged to the natural waters. Due to their low biodegradability they pose serious pollution problems if untreated. In this work, decolourization studies of Acid Red 1 (AR1) by laccase enzym...

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Autores principales: G. Ranimol, Chinju Paul, Swetha Sunkar
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Publicado: IWA Publishing 2021
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spelling oai:doaj.org-article:01c2c8601702443daea717f51532f8102021-12-02T07:40:11ZOptimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 10273-12231996-973210.2166/wst.2021.200https://doaj.org/article/01c2c8601702443daea717f51532f8102021-11-01T00:00:00Zhttp://wst.iwaponline.com/content/84/10-11/2703https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732Azo dyes are widely used in textile industries. A significant portion of these recalcitrant dyes are being discharged to the natural waters. Due to their low biodegradability they pose serious pollution problems if untreated. In this work, decolourization studies of Acid Red 1 (AR1) by laccase enzyme immobilized onto zein–polyvinyl pyrrolidone (PVP) composite nanofiber is done. The nanofibers were characterized by scanning electron microscopy (SEM) and Fourier-transform infrared (FTIR) analysis. pH and temperature profiles of immobilized enzyme were found to be broader than its free counterpart. The Km value was found to be 0.243 mM for free laccase and 0.958 mM for immobilized laccase. Similarly, Vmax for the free enzyme was 3.572 U/mg compared to 2.48 U/mg of immobilized laccase. The relative activity of immobilized laccase was 64.91% after storage for 30 days at room temperature while it was 28.64% for free laccase. The temperature and pH for AR 1 decolorization were optimized and was found to be 60 °C and 5, respectively. Also, decolorization percentage was found to be 91.67% for immobilized laccase and 72.03% of free laccase in the presence of natural mediators like vanillin. From phytotoxicity studies it was found that the germination rate, shoot and root length was increased compared to untreated dye. Therefore, zein–PVP nanofiber immobilized laccase could be an ideal candidate for the textile dye decolorization. HIGHLIGHTS Textile effluents cause serious pollution problem in the environment.; Effectiveness of laccase enzyme in the decolorization of Acid Red-1 is studied.; Laccase was used either in the free form as well as immobilized in the zein–polyvinyl pyrrolidone nanofiber membrane.; The immobilized enzyme showed greater capacity for decolorization.;G. RanimolChinju PaulSwetha SunkarIWA Publishingarticleazo dyelaccasemediatornanofiberspvpzeinEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 84, Iss 10-11, Pp 2703-2717 (2021)
institution DOAJ
collection DOAJ
language EN
topic azo dye
laccase
mediator
nanofibers
pvp
zein
Environmental technology. Sanitary engineering
TD1-1066
spellingShingle azo dye
laccase
mediator
nanofibers
pvp
zein
Environmental technology. Sanitary engineering
TD1-1066
G. Ranimol
Chinju Paul
Swetha Sunkar
Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1
description Azo dyes are widely used in textile industries. A significant portion of these recalcitrant dyes are being discharged to the natural waters. Due to their low biodegradability they pose serious pollution problems if untreated. In this work, decolourization studies of Acid Red 1 (AR1) by laccase enzyme immobilized onto zein–polyvinyl pyrrolidone (PVP) composite nanofiber is done. The nanofibers were characterized by scanning electron microscopy (SEM) and Fourier-transform infrared (FTIR) analysis. pH and temperature profiles of immobilized enzyme were found to be broader than its free counterpart. The Km value was found to be 0.243 mM for free laccase and 0.958 mM for immobilized laccase. Similarly, Vmax for the free enzyme was 3.572 U/mg compared to 2.48 U/mg of immobilized laccase. The relative activity of immobilized laccase was 64.91% after storage for 30 days at room temperature while it was 28.64% for free laccase. The temperature and pH for AR 1 decolorization were optimized and was found to be 60 °C and 5, respectively. Also, decolorization percentage was found to be 91.67% for immobilized laccase and 72.03% of free laccase in the presence of natural mediators like vanillin. From phytotoxicity studies it was found that the germination rate, shoot and root length was increased compared to untreated dye. Therefore, zein–PVP nanofiber immobilized laccase could be an ideal candidate for the textile dye decolorization. HIGHLIGHTS Textile effluents cause serious pollution problem in the environment.; Effectiveness of laccase enzyme in the decolorization of Acid Red-1 is studied.; Laccase was used either in the free form as well as immobilized in the zein–polyvinyl pyrrolidone nanofiber membrane.; The immobilized enzyme showed greater capacity for decolorization.;
format article
author G. Ranimol
Chinju Paul
Swetha Sunkar
author_facet G. Ranimol
Chinju Paul
Swetha Sunkar
author_sort G. Ranimol
title Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1
title_short Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1
title_full Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1
title_fullStr Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1
title_full_unstemmed Optimization and efficacy studies of Laccase immobilized on Zein-Polyvinyl pyrrolidone nano fibrous membrane in decolorization of Acid Red 1
title_sort optimization and efficacy studies of laccase immobilized on zein-polyvinyl pyrrolidone nano fibrous membrane in decolorization of acid red 1
publisher IWA Publishing
publishDate 2021
url https://doaj.org/article/01c2c8601702443daea717f51532f810
work_keys_str_mv AT granimol optimizationandefficacystudiesoflaccaseimmobilizedonzeinpolyvinylpyrrolidonenanofibrousmembraneindecolorizationofacidred1
AT chinjupaul optimizationandefficacystudiesoflaccaseimmobilizedonzeinpolyvinylpyrrolidonenanofibrousmembraneindecolorizationofacidred1
AT swethasunkar optimizationandefficacystudiesoflaccaseimmobilizedonzeinpolyvinylpyrrolidonenanofibrousmembraneindecolorizationofacidred1
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