Plasma membrane-localized protein BcHIPP16 promotes the uptake of copper and cadmium in planta

Cadmium (Cd) is one of the toxic heavy metals in soil, which not only suppresses crop production but also threatens human health. In this study, we aim to clarify the biological function of Cd-related gene BcHIPP16, so as to provide potential genetic solutions to decrease the Cd levels of pak choi....

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Autores principales:	Mengyang Niu, Changjian Bao, Junyi Zhan, Xiaomeng Yue, Jianwen Zou, Nana Su, Jin Cui
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Pak choi Metal chaperone BcHIPP16 Copper Cadmium Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/01ca87323000483aba5e87613c3082dc
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spelling	oai:doaj.org-article:01ca87323000483aba5e87613c3082dc2021-11-06T04:17:23ZPlasma membrane-localized protein BcHIPP16 promotes the uptake of copper and cadmium in planta0147-651310.1016/j.ecoenv.2021.112920https://doaj.org/article/01ca87323000483aba5e87613c3082dc2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010320https://doaj.org/toc/0147-6513Cadmium (Cd) is one of the toxic heavy metals in soil, which not only suppresses crop production but also threatens human health. In this study, we aim to clarify the biological function of Cd-related gene BcHIPP16, so as to provide potential genetic solutions to decrease the Cd levels of pak choi. Tissue expression analysis showed that BcHIPP16 expressed in almost all the plant bodies. The transcriptional level of BcHIPP16 in roots was higher than that in shoots, which was significantly induced by copper (Cu) deficiency and Cd exposure conditions. Subcellular localization revealed that BcHIPP16 localized in plasma membrane. Expressing BcHIPP16 in yeast cells improved the sensitivity to Cu and Cd and improved their accumulation in yeast. Furthermore, the Cu and Cd content of Arabidopsis seedlings were increased and complemented, respectively when expressing BcHIPP16 in wild type (WT) and hip16 mutants. Non-invasive Micro-test Technology (NMT) was used to measure the real-time Cd2+ influx from the root surface of BcHIPP16 transgenic Arabidopsis lines, and the result demonstrated that BcHIPP16 promoted Cd2+ influx into Arabidopsis root cells. Taken together, our study showed that BcHIPP16 contributed to absorbing nutrient metal Cu and heavy metal Cd in planta.Mengyang NiuChangjian BaoJunyi ZhanXiaomeng YueJianwen ZouNana SuJin CuiElsevierarticlePak choiMetal chaperoneBcHIPP16CopperCadmiumEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112920- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pak choi Metal chaperone BcHIPP16 Copper Cadmium Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Pak choi Metal chaperone BcHIPP16 Copper Cadmium Environmental pollution TD172-193.5 Environmental sciences GE1-350 Mengyang Niu Changjian Bao Junyi Zhan Xiaomeng Yue Jianwen Zou Nana Su Jin Cui Plasma membrane-localized protein BcHIPP16 promotes the uptake of copper and cadmium in planta
description	Cadmium (Cd) is one of the toxic heavy metals in soil, which not only suppresses crop production but also threatens human health. In this study, we aim to clarify the biological function of Cd-related gene BcHIPP16, so as to provide potential genetic solutions to decrease the Cd levels of pak choi. Tissue expression analysis showed that BcHIPP16 expressed in almost all the plant bodies. The transcriptional level of BcHIPP16 in roots was higher than that in shoots, which was significantly induced by copper (Cu) deficiency and Cd exposure conditions. Subcellular localization revealed that BcHIPP16 localized in plasma membrane. Expressing BcHIPP16 in yeast cells improved the sensitivity to Cu and Cd and improved their accumulation in yeast. Furthermore, the Cu and Cd content of Arabidopsis seedlings were increased and complemented, respectively when expressing BcHIPP16 in wild type (WT) and hip16 mutants. Non-invasive Micro-test Technology (NMT) was used to measure the real-time Cd2+ influx from the root surface of BcHIPP16 transgenic Arabidopsis lines, and the result demonstrated that BcHIPP16 promoted Cd2+ influx into Arabidopsis root cells. Taken together, our study showed that BcHIPP16 contributed to absorbing nutrient metal Cu and heavy metal Cd in planta.
format	article
author	Mengyang Niu Changjian Bao Junyi Zhan Xiaomeng Yue Jianwen Zou Nana Su Jin Cui
author_facet	Mengyang Niu Changjian Bao Junyi Zhan Xiaomeng Yue Jianwen Zou Nana Su Jin Cui
author_sort	Mengyang Niu
title	Plasma membrane-localized protein BcHIPP16 promotes the uptake of copper and cadmium in planta
title_short	Plasma membrane-localized protein BcHIPP16 promotes the uptake of copper and cadmium in planta
title_full	Plasma membrane-localized protein BcHIPP16 promotes the uptake of copper and cadmium in planta
title_fullStr	Plasma membrane-localized protein BcHIPP16 promotes the uptake of copper and cadmium in planta
title_full_unstemmed	Plasma membrane-localized protein BcHIPP16 promotes the uptake of copper and cadmium in planta
title_sort	plasma membrane-localized protein bchipp16 promotes the uptake of copper and cadmium in planta
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/01ca87323000483aba5e87613c3082dc
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Plasma membrane-localized protein BcHIPP16 promotes the uptake of copper and cadmium in planta

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