Metabolomic Characterization of Fatty Acids in Patients With Coronary Artery Ectasias

Background: We used a targeted metabolomics approach to identify fatty acid (FA) metabolites that distinguished patients with coronary artery ectasia (CAE) from healthy Controls and patients with coronary artery disease (CAD).Materials and methods: Two hundred fifty-two human subjects were enrolled...

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Autores principales:	Tianlong Liu, Yingying Sun, Hao Li, Haochen Xu, Ning Xiao, Xuliang Wang, Li Song, Congxia Bai, Hongyan Wen, Jing Ge, Yinhui Zhang, Weihua Song, Jingzhou Chen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	plasma metabolomic profiles coronary artery ectasia biomarkers coronary artery disease fatty acid metabolites Physiology QP1-981
Acceso en línea:	https://doaj.org/article/01d4cd4f5f0d46a29dd1e1fc1fd525d4
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spelling	oai:doaj.org-article:01d4cd4f5f0d46a29dd1e1fc1fd525d42021-11-19T07:01:28ZMetabolomic Characterization of Fatty Acids in Patients With Coronary Artery Ectasias1664-042X10.3389/fphys.2021.770223https://doaj.org/article/01d4cd4f5f0d46a29dd1e1fc1fd525d42021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.770223/fullhttps://doaj.org/toc/1664-042XBackground: We used a targeted metabolomics approach to identify fatty acid (FA) metabolites that distinguished patients with coronary artery ectasia (CAE) from healthy Controls and patients with coronary artery disease (CAD).Materials and methods: Two hundred fifty-two human subjects were enrolled in our study, such as patients with CAE, patients with CAD, and Controls. All the subjects were diagnosed by coronary angiography. Plasma metabolomic profiles of FAs were determined by an ultra-high-performance liquid chromatography coupled to triple quadrupole mass spectrometric (UPLC-QqQ-MS/MS).Results: Ninety-nine plasma metabolites were profiled in the discovery sets (n = 72), such as 35 metabolites of arachidonic acid (AA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), 10 FAs, and 54 phospholipids. Among these metabolites, 36 metabolites of AA, EPA, and DHA showed the largest difference between CAE and Controls or CAD. 12-hydroxyeicosatetraenoic acid (12-HETE), 17(S)-hydroxydocosahexaenoic acid (17-HDoHE), EPA, AA, and 5-HETE were defined as a biomarker panel in peripheral blood to distinguish CAE from CAD and Controls in a discovery set (n = 72) and a validation set (n = 180). This biomarker panel had a better diagnostic performance than metabolite alone in differentiating CAE from Controls and CAD. The areas under the ROC curve of the biomarker panel were 0.991 and 0.836 for CAE versus Controls and 1.00 and 0.904 for CAE versus CAD in the discovery and validation sets, respectively.Conclusions: Our findings revealed that the metabolic profiles of FAs in the plasma from patients with CAE can be distinguished from those of Controls and CAD. Differences in FAs metabolites may help to interpret pathological mechanisms of CAE.Tianlong LiuTianlong LiuYingying SunHao LiHaochen XuNing XiaoXuliang WangLi SongCongxia BaiHongyan WenJing GeYinhui ZhangWeihua SongJingzhou ChenFrontiers Media S.A.articleplasma metabolomic profilescoronary artery ectasiabiomarkerscoronary artery diseasefatty acid metabolitesPhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plasma metabolomic profiles coronary artery ectasia biomarkers coronary artery disease fatty acid metabolites Physiology QP1-981
spellingShingle	plasma metabolomic profiles coronary artery ectasia biomarkers coronary artery disease fatty acid metabolites Physiology QP1-981 Tianlong Liu Tianlong Liu Yingying Sun Hao Li Haochen Xu Ning Xiao Xuliang Wang Li Song Congxia Bai Hongyan Wen Jing Ge Yinhui Zhang Weihua Song Jingzhou Chen Metabolomic Characterization of Fatty Acids in Patients With Coronary Artery Ectasias
description	Background: We used a targeted metabolomics approach to identify fatty acid (FA) metabolites that distinguished patients with coronary artery ectasia (CAE) from healthy Controls and patients with coronary artery disease (CAD).Materials and methods: Two hundred fifty-two human subjects were enrolled in our study, such as patients with CAE, patients with CAD, and Controls. All the subjects were diagnosed by coronary angiography. Plasma metabolomic profiles of FAs were determined by an ultra-high-performance liquid chromatography coupled to triple quadrupole mass spectrometric (UPLC-QqQ-MS/MS).Results: Ninety-nine plasma metabolites were profiled in the discovery sets (n = 72), such as 35 metabolites of arachidonic acid (AA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), 10 FAs, and 54 phospholipids. Among these metabolites, 36 metabolites of AA, EPA, and DHA showed the largest difference between CAE and Controls or CAD. 12-hydroxyeicosatetraenoic acid (12-HETE), 17(S)-hydroxydocosahexaenoic acid (17-HDoHE), EPA, AA, and 5-HETE were defined as a biomarker panel in peripheral blood to distinguish CAE from CAD and Controls in a discovery set (n = 72) and a validation set (n = 180). This biomarker panel had a better diagnostic performance than metabolite alone in differentiating CAE from Controls and CAD. The areas under the ROC curve of the biomarker panel were 0.991 and 0.836 for CAE versus Controls and 1.00 and 0.904 for CAE versus CAD in the discovery and validation sets, respectively.Conclusions: Our findings revealed that the metabolic profiles of FAs in the plasma from patients with CAE can be distinguished from those of Controls and CAD. Differences in FAs metabolites may help to interpret pathological mechanisms of CAE.
format	article
author	Tianlong Liu Tianlong Liu Yingying Sun Hao Li Haochen Xu Ning Xiao Xuliang Wang Li Song Congxia Bai Hongyan Wen Jing Ge Yinhui Zhang Weihua Song Jingzhou Chen
author_facet	Tianlong Liu Tianlong Liu Yingying Sun Hao Li Haochen Xu Ning Xiao Xuliang Wang Li Song Congxia Bai Hongyan Wen Jing Ge Yinhui Zhang Weihua Song Jingzhou Chen
author_sort	Tianlong Liu
title	Metabolomic Characterization of Fatty Acids in Patients With Coronary Artery Ectasias
title_short	Metabolomic Characterization of Fatty Acids in Patients With Coronary Artery Ectasias
title_full	Metabolomic Characterization of Fatty Acids in Patients With Coronary Artery Ectasias
title_fullStr	Metabolomic Characterization of Fatty Acids in Patients With Coronary Artery Ectasias
title_full_unstemmed	Metabolomic Characterization of Fatty Acids in Patients With Coronary Artery Ectasias
title_sort	metabolomic characterization of fatty acids in patients with coronary artery ectasias
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/01d4cd4f5f0d46a29dd1e1fc1fd525d4
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Metabolomic Characterization of Fatty Acids in Patients With Coronary Artery Ectasias

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