Bacterial adhesion inhibitor prevents infection in a rodent surgical incision model

Surgical site infection risk continues to increase due to lack of efficacy in current standard of care drugs. New methods to treat or prevent antibiotic-resistant bacterial infections are needed. Multivalent Adhesion Molecules (MAM) are bacterial adhesins required for virulence. We developed a bacte...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	R. M. Huebinger, D. H. Do, D. L. Carlson, X. Yao, D. H. Stones, M. De Souza Santos, D. P. Vaz, E. Keen, S. E. Wolf, JP Minei, K. P. Francis, K. Orth, A. M. Krachler
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	surgical infection incision laceration bacterial adhesion anti-virulence pseudomonas staphylococcus Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/01dfe1cd1ef347c5835f50de064df194
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:01dfe1cd1ef347c5835f50de064df194
record_format	dspace
spelling	oai:doaj.org-article:01dfe1cd1ef347c5835f50de064df1942021-11-17T14:21:58ZBacterial adhesion inhibitor prevents infection in a rodent surgical incision model2150-55942150-560810.1080/21505594.2020.1772652https://doaj.org/article/01dfe1cd1ef347c5835f50de064df1942020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1772652https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Surgical site infection risk continues to increase due to lack of efficacy in current standard of care drugs. New methods to treat or prevent antibiotic-resistant bacterial infections are needed. Multivalent Adhesion Molecules (MAM) are bacterial adhesins required for virulence. We developed a bacterial adhesion inhibitor using recombinant MAM fragment bound to polymer scaffold, mimicking MAM7 display on the bacterial surface. Here, we test MAM7 inhibitor efficacy to prevent Gram-positive and Gram-negative infections. Using a rodent model of surgical infection, incision sites were infected with antibiotic-resistant bioluminescent strains of Staphylococcus aureus or Pseudomonas aeruginosa. Infections were treated with MAM7 inhibitor or control suspension. Bacterial abundance was quantified for nine days post infection. Inflammatory responses and histology were characterized using fixed tissue sections. MAM7 inhibitor treatment decreased burden of S. aureus and P. aeruginosa below detection threshold. Bacterial load of groups treated with control were significantly higher than MAM7 inhibitor-treated groups. Treatment with inhibitor reduced colonization of clinically-relevant pathogens in an in vivo model of surgical infection. Use of MAM7 inhibitor to block initial adhesion of bacteria to tissue in surgical incisions may reduce infection rates, presenting a strategy to mitigate overuse of antibiotics to prevent surgical site infections.R. M. HuebingerD. H. DoD. L. CarlsonX. YaoD. H. StonesM. De Souza SantosD. P. VazE. KeenS. E. WolfJP MineiK. P. FrancisK. OrthA. M. KrachlerTaylor & Francis Grouparticlesurgical infectionincisionlacerationbacterial adhesionanti-virulencepseudomonasstaphylococcusInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 695-706 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	surgical infection incision laceration bacterial adhesion anti-virulence pseudomonas staphylococcus Infectious and parasitic diseases RC109-216
spellingShingle	surgical infection incision laceration bacterial adhesion anti-virulence pseudomonas staphylococcus Infectious and parasitic diseases RC109-216 R. M. Huebinger D. H. Do D. L. Carlson X. Yao D. H. Stones M. De Souza Santos D. P. Vaz E. Keen S. E. Wolf JP Minei K. P. Francis K. Orth A. M. Krachler Bacterial adhesion inhibitor prevents infection in a rodent surgical incision model
description	Surgical site infection risk continues to increase due to lack of efficacy in current standard of care drugs. New methods to treat or prevent antibiotic-resistant bacterial infections are needed. Multivalent Adhesion Molecules (MAM) are bacterial adhesins required for virulence. We developed a bacterial adhesion inhibitor using recombinant MAM fragment bound to polymer scaffold, mimicking MAM7 display on the bacterial surface. Here, we test MAM7 inhibitor efficacy to prevent Gram-positive and Gram-negative infections. Using a rodent model of surgical infection, incision sites were infected with antibiotic-resistant bioluminescent strains of Staphylococcus aureus or Pseudomonas aeruginosa. Infections were treated with MAM7 inhibitor or control suspension. Bacterial abundance was quantified for nine days post infection. Inflammatory responses and histology were characterized using fixed tissue sections. MAM7 inhibitor treatment decreased burden of S. aureus and P. aeruginosa below detection threshold. Bacterial load of groups treated with control were significantly higher than MAM7 inhibitor-treated groups. Treatment with inhibitor reduced colonization of clinically-relevant pathogens in an in vivo model of surgical infection. Use of MAM7 inhibitor to block initial adhesion of bacteria to tissue in surgical incisions may reduce infection rates, presenting a strategy to mitigate overuse of antibiotics to prevent surgical site infections.
format	article
author	R. M. Huebinger D. H. Do D. L. Carlson X. Yao D. H. Stones M. De Souza Santos D. P. Vaz E. Keen S. E. Wolf JP Minei K. P. Francis K. Orth A. M. Krachler
author_facet	R. M. Huebinger D. H. Do D. L. Carlson X. Yao D. H. Stones M. De Souza Santos D. P. Vaz E. Keen S. E. Wolf JP Minei K. P. Francis K. Orth A. M. Krachler
author_sort	R. M. Huebinger
title	Bacterial adhesion inhibitor prevents infection in a rodent surgical incision model
title_short	Bacterial adhesion inhibitor prevents infection in a rodent surgical incision model
title_full	Bacterial adhesion inhibitor prevents infection in a rodent surgical incision model
title_fullStr	Bacterial adhesion inhibitor prevents infection in a rodent surgical incision model
title_full_unstemmed	Bacterial adhesion inhibitor prevents infection in a rodent surgical incision model
title_sort	bacterial adhesion inhibitor prevents infection in a rodent surgical incision model
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/01dfe1cd1ef347c5835f50de064df194
work_keys_str_mv	AT rmhuebinger bacterialadhesioninhibitorpreventsinfectioninarodentsurgicalincisionmodel AT dhdo bacterialadhesioninhibitorpreventsinfectioninarodentsurgicalincisionmodel AT dlcarlson bacterialadhesioninhibitorpreventsinfectioninarodentsurgicalincisionmodel AT xyao bacterialadhesioninhibitorpreventsinfectioninarodentsurgicalincisionmodel AT dhstones bacterialadhesioninhibitorpreventsinfectioninarodentsurgicalincisionmodel AT mdesouzasantos bacterialadhesioninhibitorpreventsinfectioninarodentsurgicalincisionmodel AT dpvaz bacterialadhesioninhibitorpreventsinfectioninarodentsurgicalincisionmodel AT ekeen bacterialadhesioninhibitorpreventsinfectioninarodentsurgicalincisionmodel AT sewolf bacterialadhesioninhibitorpreventsinfectioninarodentsurgicalincisionmodel AT jpminei bacterialadhesioninhibitorpreventsinfectioninarodentsurgicalincisionmodel AT kpfrancis bacterialadhesioninhibitorpreventsinfectioninarodentsurgicalincisionmodel AT korth bacterialadhesioninhibitorpreventsinfectioninarodentsurgicalincisionmodel AT amkrachler bacterialadhesioninhibitorpreventsinfectioninarodentsurgicalincisionmodel
_version_	1718425459298926592

Bacterial adhesion inhibitor prevents infection in a rodent surgical incision model

Ejemplares similares