Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging

Abstract In contrast to hypertrophic cardiomyopathy, there has been reported no specific pattern of cardiomyocyte array in dilated cardiomyopathy (DCM), partially because lack of alignment assessment in a three-dimensional (3D) manner. Here we have established a novel method to evaluate cardiomyocyt...

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Autores principales:	Kiyoshi Masuyama, Tomoaki Higo, Jong-Kook Lee, Ryohei Matsuura, Ian Jones, Chris Bakal, Shuichiro Higo, Sachio Morimoto, Shigeru Miyagawa, Yoshiki Sawa, Yasushi Sakata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/01e05629efd64411baf626de8aae2df3
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spelling	oai:doaj.org-article:01e05629efd64411baf626de8aae2df32021-12-02T16:17:17ZHomogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging10.1038/s41598-021-94100-z2045-2322https://doaj.org/article/01e05629efd64411baf626de8aae2df32021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94100-zhttps://doaj.org/toc/2045-2322Abstract In contrast to hypertrophic cardiomyopathy, there has been reported no specific pattern of cardiomyocyte array in dilated cardiomyopathy (DCM), partially because lack of alignment assessment in a three-dimensional (3D) manner. Here we have established a novel method to evaluate cardiomyocyte alignment in 3D using intravital heart imaging and demonstrated homogeneous alignment in DCM mice. Whilst cardiomyocytes of control mice changed their alignment by every layer in 3D and position twistedly even in a single layer, termed myocyte twist, cardiomyocytes of DCM mice aligned homogeneously both in two-dimensional (2D) and in 3D and lost myocyte twist. Manipulation of cultured cardiomyocyte toward homogeneously aligned increased their contractility, suggesting that homogeneous alignment in DCM mice is due to a sort of alignment remodelling as a way to compensate cardiac dysfunction. Our findings provide the first intravital evidence of cardiomyocyte alignment and will bring new insights into understanding the mechanism of heart failure.Kiyoshi MasuyamaTomoaki HigoJong-Kook LeeRyohei MatsuuraIan JonesChris BakalShuichiro HigoSachio MorimotoShigeru MiyagawaYoshiki SawaYasushi SakataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kiyoshi Masuyama Tomoaki Higo Jong-Kook Lee Ryohei Matsuura Ian Jones Chris Bakal Shuichiro Higo Sachio Morimoto Shigeru Miyagawa Yoshiki Sawa Yasushi Sakata Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging
description	Abstract In contrast to hypertrophic cardiomyopathy, there has been reported no specific pattern of cardiomyocyte array in dilated cardiomyopathy (DCM), partially because lack of alignment assessment in a three-dimensional (3D) manner. Here we have established a novel method to evaluate cardiomyocyte alignment in 3D using intravital heart imaging and demonstrated homogeneous alignment in DCM mice. Whilst cardiomyocytes of control mice changed their alignment by every layer in 3D and position twistedly even in a single layer, termed myocyte twist, cardiomyocytes of DCM mice aligned homogeneously both in two-dimensional (2D) and in 3D and lost myocyte twist. Manipulation of cultured cardiomyocyte toward homogeneously aligned increased their contractility, suggesting that homogeneous alignment in DCM mice is due to a sort of alignment remodelling as a way to compensate cardiac dysfunction. Our findings provide the first intravital evidence of cardiomyocyte alignment and will bring new insights into understanding the mechanism of heart failure.
format	article
author	Kiyoshi Masuyama Tomoaki Higo Jong-Kook Lee Ryohei Matsuura Ian Jones Chris Bakal Shuichiro Higo Sachio Morimoto Shigeru Miyagawa Yoshiki Sawa Yasushi Sakata
author_facet	Kiyoshi Masuyama Tomoaki Higo Jong-Kook Lee Ryohei Matsuura Ian Jones Chris Bakal Shuichiro Higo Sachio Morimoto Shigeru Miyagawa Yoshiki Sawa Yasushi Sakata
author_sort	Kiyoshi Masuyama
title	Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging
title_short	Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging
title_full	Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging
title_fullStr	Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging
title_full_unstemmed	Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging
title_sort	homogeneous 2d and 3d alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/01e05629efd64411baf626de8aae2df3
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Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging

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