Integrated Transcriptome and Multiple Activated Pathways in Endometrial Cancer

Because the incidence of endometrial cancer is notably increasing worldwide, it has become the leading gynecologic cancer in the United States. Standard treatment results in the loss of reproductive function in women of childbearing age. Furthermore, advanced cancer stages are associated with poor o...

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Autores principales:	Qi Jin, Xiaohua Jiang, Xin Du, Weiping Hu, Shun Bai, Xian Wang, Bo Xu, Weidong Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	endometrial cancer transcriptome sequencing enrichment analysis TGF- β pathway PI3K-Akt pathway Genetics QH426-470
Acceso en línea:	https://doaj.org/article/01e057bbc9314201a1fd794b45eae57e
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spelling	oai:doaj.org-article:01e057bbc9314201a1fd794b45eae57e2021-12-03T06:46:18ZIntegrated Transcriptome and Multiple Activated Pathways in Endometrial Cancer1664-802110.3389/fgene.2021.680331https://doaj.org/article/01e057bbc9314201a1fd794b45eae57e2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.680331/fullhttps://doaj.org/toc/1664-8021Because the incidence of endometrial cancer is notably increasing worldwide, it has become the leading gynecologic cancer in the United States. Standard treatment results in the loss of reproductive function in women of childbearing age. Furthermore, advanced cancer stages are associated with poor overall survival. The aim of this study was to explore the abnormal expression profile of genes during the development of endometrial cancer, which is essential to provide a better understanding of the mechanisms involved. Five pairs of endometrial cancer tissues and normal endometrial tissues were subjected to next-generation transcriptome sequencing technology. Quantitative real-time PCR (RT-qPCR) was performed to validate the expression profile of key differentially expressed genes (2.0-fold change, adj. p < 0.05) (DEGs) identified in the RNA-seq result. GO and KEGG pathways were used for bioinformatic analyses. The transcriptomic sequencing results showed 1153 DEGs, including 673 upregulated and 480 downregulated genes, in the EC specimens. Decreased expression of ID1, IGF1, GDF7, SMAD9, TGF-beta and WNT4, as well as GDF5, INHBA and ERBB4 overexpression, were confirmed in EC using RT-qPCR. Additionally, EC tissue exhibited marked enrichment in genes promoting cellular adhesion, proliferation, migration and plasma membrane. KEGG analysis revealed changes in various pathways, such as the TGF-beta, PI3K-Akt, Wnt, and estrogen pathways. Our data describe the molecular events involved in the pathogenesis of EC, which may be potential diagnostic markers and targets of therapeutic interventions.Qi JinQi JinXiaohua JiangXin DuWeiping HuShun BaiXian WangBo XuWeidong ZhaoWeidong ZhaoFrontiers Media S.A.articleendometrial cancertranscriptome sequencingenrichment analysisTGF- β pathwayPI3K-Akt pathwayGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endometrial cancer transcriptome sequencing enrichment analysis TGF- β pathway PI3K-Akt pathway Genetics QH426-470
spellingShingle	endometrial cancer transcriptome sequencing enrichment analysis TGF- β pathway PI3K-Akt pathway Genetics QH426-470 Qi Jin Qi Jin Xiaohua Jiang Xin Du Weiping Hu Shun Bai Xian Wang Bo Xu Weidong Zhao Weidong Zhao Integrated Transcriptome and Multiple Activated Pathways in Endometrial Cancer
description	Because the incidence of endometrial cancer is notably increasing worldwide, it has become the leading gynecologic cancer in the United States. Standard treatment results in the loss of reproductive function in women of childbearing age. Furthermore, advanced cancer stages are associated with poor overall survival. The aim of this study was to explore the abnormal expression profile of genes during the development of endometrial cancer, which is essential to provide a better understanding of the mechanisms involved. Five pairs of endometrial cancer tissues and normal endometrial tissues were subjected to next-generation transcriptome sequencing technology. Quantitative real-time PCR (RT-qPCR) was performed to validate the expression profile of key differentially expressed genes (2.0-fold change, adj. p < 0.05) (DEGs) identified in the RNA-seq result. GO and KEGG pathways were used for bioinformatic analyses. The transcriptomic sequencing results showed 1153 DEGs, including 673 upregulated and 480 downregulated genes, in the EC specimens. Decreased expression of ID1, IGF1, GDF7, SMAD9, TGF-beta and WNT4, as well as GDF5, INHBA and ERBB4 overexpression, were confirmed in EC using RT-qPCR. Additionally, EC tissue exhibited marked enrichment in genes promoting cellular adhesion, proliferation, migration and plasma membrane. KEGG analysis revealed changes in various pathways, such as the TGF-beta, PI3K-Akt, Wnt, and estrogen pathways. Our data describe the molecular events involved in the pathogenesis of EC, which may be potential diagnostic markers and targets of therapeutic interventions.
format	article
author	Qi Jin Qi Jin Xiaohua Jiang Xin Du Weiping Hu Shun Bai Xian Wang Bo Xu Weidong Zhao Weidong Zhao
author_facet	Qi Jin Qi Jin Xiaohua Jiang Xin Du Weiping Hu Shun Bai Xian Wang Bo Xu Weidong Zhao Weidong Zhao
author_sort	Qi Jin
title	Integrated Transcriptome and Multiple Activated Pathways in Endometrial Cancer
title_short	Integrated Transcriptome and Multiple Activated Pathways in Endometrial Cancer
title_full	Integrated Transcriptome and Multiple Activated Pathways in Endometrial Cancer
title_fullStr	Integrated Transcriptome and Multiple Activated Pathways in Endometrial Cancer
title_full_unstemmed	Integrated Transcriptome and Multiple Activated Pathways in Endometrial Cancer
title_sort	integrated transcriptome and multiple activated pathways in endometrial cancer
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/01e057bbc9314201a1fd794b45eae57e
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Integrated Transcriptome and Multiple Activated Pathways in Endometrial Cancer

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