Comparative transcriptomics between Drosophila mojavensis and D. arizonae reveals transgressive gene expression and underexpression of spermatogenesis-related genes in hybrid testes

Abstract Interspecific hybridization is a stressful condition that can lead to sterility and/or inviability through improper gene regulation in Drosophila species with a high divergence time. However, the extent of these abnormalities in hybrids of recently diverging species is not well known. Some...

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Autores principales:	Cecilia A. Banho, Vincent Mérel, Thiago Y. K. Oliveira, Claudia M. A. Carareto, Cristina Vieira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/01e5027d37944840968877a907c8fbdd
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spelling	oai:doaj.org-article:01e5027d37944840968877a907c8fbdd2021-12-02T17:01:43ZComparative transcriptomics between Drosophila mojavensis and D. arizonae reveals transgressive gene expression and underexpression of spermatogenesis-related genes in hybrid testes10.1038/s41598-021-89366-22045-2322https://doaj.org/article/01e5027d37944840968877a907c8fbdd2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89366-2https://doaj.org/toc/2045-2322Abstract Interspecific hybridization is a stressful condition that can lead to sterility and/or inviability through improper gene regulation in Drosophila species with a high divergence time. However, the extent of these abnormalities in hybrids of recently diverging species is not well known. Some studies have shown that in Drosophila, the mechanisms of postzygotic isolation may evolve more rapidly in males than in females and that the degree of viability and sterility is associated with the genetic distance between species. Here, we used transcriptomic comparisons between two Drosophila mojavensis subspecies and D. arizonae (repleta group, Drosophila) and identified greater differential gene expression in testes than in ovaries. We tested the hypothesis that the severity of the interspecies hybrid phenotype is associated with the degree of gene misregulation. We showed limited gene misregulation in fertile females and an increase in the amount of misregulation in males with more severe sterile phenotypes (motile vs. amotile sperm). In addition, for these hybrids, we identified candidate genes that were mostly associated with spermatogenesis dysfunction.Cecilia A. BanhoVincent MérelThiago Y. K. OliveiraClaudia M. A. CararetoCristina VieiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cecilia A. Banho Vincent Mérel Thiago Y. K. Oliveira Claudia M. A. Carareto Cristina Vieira Comparative transcriptomics between Drosophila mojavensis and D. arizonae reveals transgressive gene expression and underexpression of spermatogenesis-related genes in hybrid testes
description	Abstract Interspecific hybridization is a stressful condition that can lead to sterility and/or inviability through improper gene regulation in Drosophila species with a high divergence time. However, the extent of these abnormalities in hybrids of recently diverging species is not well known. Some studies have shown that in Drosophila, the mechanisms of postzygotic isolation may evolve more rapidly in males than in females and that the degree of viability and sterility is associated with the genetic distance between species. Here, we used transcriptomic comparisons between two Drosophila mojavensis subspecies and D. arizonae (repleta group, Drosophila) and identified greater differential gene expression in testes than in ovaries. We tested the hypothesis that the severity of the interspecies hybrid phenotype is associated with the degree of gene misregulation. We showed limited gene misregulation in fertile females and an increase in the amount of misregulation in males with more severe sterile phenotypes (motile vs. amotile sperm). In addition, for these hybrids, we identified candidate genes that were mostly associated with spermatogenesis dysfunction.
format	article
author	Cecilia A. Banho Vincent Mérel Thiago Y. K. Oliveira Claudia M. A. Carareto Cristina Vieira
author_facet	Cecilia A. Banho Vincent Mérel Thiago Y. K. Oliveira Claudia M. A. Carareto Cristina Vieira
author_sort	Cecilia A. Banho
title	Comparative transcriptomics between Drosophila mojavensis and D. arizonae reveals transgressive gene expression and underexpression of spermatogenesis-related genes in hybrid testes
title_short	Comparative transcriptomics between Drosophila mojavensis and D. arizonae reveals transgressive gene expression and underexpression of spermatogenesis-related genes in hybrid testes
title_full	Comparative transcriptomics between Drosophila mojavensis and D. arizonae reveals transgressive gene expression and underexpression of spermatogenesis-related genes in hybrid testes
title_fullStr	Comparative transcriptomics between Drosophila mojavensis and D. arizonae reveals transgressive gene expression and underexpression of spermatogenesis-related genes in hybrid testes
title_full_unstemmed	Comparative transcriptomics between Drosophila mojavensis and D. arizonae reveals transgressive gene expression and underexpression of spermatogenesis-related genes in hybrid testes
title_sort	comparative transcriptomics between drosophila mojavensis and d. arizonae reveals transgressive gene expression and underexpression of spermatogenesis-related genes in hybrid testes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/01e5027d37944840968877a907c8fbdd
work_keys_str_mv	AT ceciliaabanho comparativetranscriptomicsbetweendrosophilamojavensisanddarizonaerevealstransgressivegeneexpressionandunderexpressionofspermatogenesisrelatedgenesinhybridtestes AT vincentmerel comparativetranscriptomicsbetweendrosophilamojavensisanddarizonaerevealstransgressivegeneexpressionandunderexpressionofspermatogenesisrelatedgenesinhybridtestes AT thiagoykoliveira comparativetranscriptomicsbetweendrosophilamojavensisanddarizonaerevealstransgressivegeneexpressionandunderexpressionofspermatogenesisrelatedgenesinhybridtestes AT claudiamacarareto comparativetranscriptomicsbetweendrosophilamojavensisanddarizonaerevealstransgressivegeneexpressionandunderexpressionofspermatogenesisrelatedgenesinhybridtestes AT cristinavieira comparativetranscriptomicsbetweendrosophilamojavensisanddarizonaerevealstransgressivegeneexpressionandunderexpressionofspermatogenesisrelatedgenesinhybridtestes
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Comparative transcriptomics between Drosophila mojavensis and D. arizonae reveals transgressive gene expression and underexpression of spermatogenesis-related genes in hybrid testes

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