Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line

Abstract Compact myelin comprises most of the dry weight of myelin, and its insulative nature is the basis for saltatory conduction of nerve impulses. The major dense line (MDL) is a 3-nm compartment between two cytoplasmic leaflets of stacked myelin membranes, mostly occupied by a myelin basic prot...

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Autores principales:	Arne Raasakka, Salla Ruskamo, Julia Kowal, Robert Barker, Anne Baumann, Anne Martel, Jussi Tuusa, Matti Myllykoski, Jochen Bürck, Anne S. Ulrich, Henning Stahlberg, Petri Kursula
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/01e8e8607aeb49f7a8030653f1e6cce9
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spelling	oai:doaj.org-article:01e8e8607aeb49f7a8030653f1e6cce92021-12-02T12:31:54ZMembrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line10.1038/s41598-017-05364-32045-2322https://doaj.org/article/01e8e8607aeb49f7a8030653f1e6cce92017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05364-3https://doaj.org/toc/2045-2322Abstract Compact myelin comprises most of the dry weight of myelin, and its insulative nature is the basis for saltatory conduction of nerve impulses. The major dense line (MDL) is a 3-nm compartment between two cytoplasmic leaflets of stacked myelin membranes, mostly occupied by a myelin basic protein (MBP) phase. MBP is an abundant myelin protein involved in demyelinating diseases, such as multiple sclerosis. The association of MBP with lipid membranes has been studied for decades, but the MBP-driven formation of the MDL remains elusive at the biomolecular level. We employed complementary biophysical methods, including atomic force microscopy, cryo-electron microscopy, and neutron scattering, to investigate the formation of membrane stacks all the way from MBP binding onto a single membrane leaflet to the organisation of a stable MDL. Our results support the formation of an amorphous protein phase of MBP between two membrane bilayers and provide a molecular model for MDL formation during myelination, which is of importance when understanding myelin assembly and demyelinating conditions.Arne RaasakkaSalla RuskamoJulia KowalRobert BarkerAnne BaumannAnne MartelJussi TuusaMatti MyllykoskiJochen BürckAnne S. UlrichHenning StahlbergPetri KursulaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-18 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arne Raasakka Salla Ruskamo Julia Kowal Robert Barker Anne Baumann Anne Martel Jussi Tuusa Matti Myllykoski Jochen Bürck Anne S. Ulrich Henning Stahlberg Petri Kursula Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line
description	Abstract Compact myelin comprises most of the dry weight of myelin, and its insulative nature is the basis for saltatory conduction of nerve impulses. The major dense line (MDL) is a 3-nm compartment between two cytoplasmic leaflets of stacked myelin membranes, mostly occupied by a myelin basic protein (MBP) phase. MBP is an abundant myelin protein involved in demyelinating diseases, such as multiple sclerosis. The association of MBP with lipid membranes has been studied for decades, but the MBP-driven formation of the MDL remains elusive at the biomolecular level. We employed complementary biophysical methods, including atomic force microscopy, cryo-electron microscopy, and neutron scattering, to investigate the formation of membrane stacks all the way from MBP binding onto a single membrane leaflet to the organisation of a stable MDL. Our results support the formation of an amorphous protein phase of MBP between two membrane bilayers and provide a molecular model for MDL formation during myelination, which is of importance when understanding myelin assembly and demyelinating conditions.
format	article
author	Arne Raasakka Salla Ruskamo Julia Kowal Robert Barker Anne Baumann Anne Martel Jussi Tuusa Matti Myllykoski Jochen Bürck Anne S. Ulrich Henning Stahlberg Petri Kursula
author_facet	Arne Raasakka Salla Ruskamo Julia Kowal Robert Barker Anne Baumann Anne Martel Jussi Tuusa Matti Myllykoski Jochen Bürck Anne S. Ulrich Henning Stahlberg Petri Kursula
author_sort	Arne Raasakka
title	Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line
title_short	Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line
title_full	Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line
title_fullStr	Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line
title_full_unstemmed	Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line
title_sort	membrane association landscape of myelin basic protein portrays formation of the myelin major dense line
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/01e8e8607aeb49f7a8030653f1e6cce9
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Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line

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