Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation

Abstract Bacterial lipopolysaccharides (LPS) activate the TRPA1 cation channels in sensory neurons, leading to acute pain and inflammation in mice and to aversive behaviors in fruit flies. However, the precise mechanisms underlying this effect remain elusive. Here we assessed the hypothesis that TRP...

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Autores principales:	Justyna B. Startek, Karel Talavera, Thomas Voets, Yeranddy A. Alpizar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/01f0e445cd9540058b3e232f789a390d
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spelling	oai:doaj.org-article:01f0e445cd9540058b3e232f789a390d2021-12-02T15:07:59ZDifferential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation10.1038/s41598-018-30534-22045-2322https://doaj.org/article/01f0e445cd9540058b3e232f789a390d2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30534-2https://doaj.org/toc/2045-2322Abstract Bacterial lipopolysaccharides (LPS) activate the TRPA1 cation channels in sensory neurons, leading to acute pain and inflammation in mice and to aversive behaviors in fruit flies. However, the precise mechanisms underlying this effect remain elusive. Here we assessed the hypothesis that TRPA1 is activated by mechanical perturbations induced upon LPS insertion in the plasma membrane. We asked whether the effects of different LPS on TRPA1 relate to their ability to induce mechanical alterations in artificial and cellular membranes. We found that LPS from E. coli, but not from S. minnesota, activates TRPA1. We then assessed the effects of these LPS on lipid membranes using dyes whose fluorescence properties change upon alteration of the local lipid environment. E. coli LPS was more effective than S. minnesota LPS in shifting Laurdan’s emission spectrum towards lower wavelengths, increasing the fluorescence anisotropy of diphenylhexatriene and reducing the fluorescence intensity of merocyanine 540. These data indicate that E. coli LPS induces stronger changes in the local lipid environment than S. minnesota LPS, paralleling its distinct ability to activate TRPA1. Our findings indicate that LPS activate TRPA1 by producing mechanical perturbations in the plasma membrane and suggest that TRPA1-mediated chemosensation may result from primary mechanosensory mechanisms.Justyna B. StartekKarel TalaveraThomas VoetsYeranddy A. AlpizarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Justyna B. Startek Karel Talavera Thomas Voets Yeranddy A. Alpizar Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation
description	Abstract Bacterial lipopolysaccharides (LPS) activate the TRPA1 cation channels in sensory neurons, leading to acute pain and inflammation in mice and to aversive behaviors in fruit flies. However, the precise mechanisms underlying this effect remain elusive. Here we assessed the hypothesis that TRPA1 is activated by mechanical perturbations induced upon LPS insertion in the plasma membrane. We asked whether the effects of different LPS on TRPA1 relate to their ability to induce mechanical alterations in artificial and cellular membranes. We found that LPS from E. coli, but not from S. minnesota, activates TRPA1. We then assessed the effects of these LPS on lipid membranes using dyes whose fluorescence properties change upon alteration of the local lipid environment. E. coli LPS was more effective than S. minnesota LPS in shifting Laurdan’s emission spectrum towards lower wavelengths, increasing the fluorescence anisotropy of diphenylhexatriene and reducing the fluorescence intensity of merocyanine 540. These data indicate that E. coli LPS induces stronger changes in the local lipid environment than S. minnesota LPS, paralleling its distinct ability to activate TRPA1. Our findings indicate that LPS activate TRPA1 by producing mechanical perturbations in the plasma membrane and suggest that TRPA1-mediated chemosensation may result from primary mechanosensory mechanisms.
format	article
author	Justyna B. Startek Karel Talavera Thomas Voets Yeranddy A. Alpizar
author_facet	Justyna B. Startek Karel Talavera Thomas Voets Yeranddy A. Alpizar
author_sort	Justyna B. Startek
title	Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation
title_short	Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation
title_full	Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation
title_fullStr	Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation
title_full_unstemmed	Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation
title_sort	differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for trpa1-mediated chemosensation
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/01f0e445cd9540058b3e232f789a390d
work_keys_str_mv	AT justynabstartek differentialinteractionsofbacteriallipopolysaccharideswithlipidmembranesimplicationsfortrpa1mediatedchemosensation AT kareltalavera differentialinteractionsofbacteriallipopolysaccharideswithlipidmembranesimplicationsfortrpa1mediatedchemosensation AT thomasvoets differentialinteractionsofbacteriallipopolysaccharideswithlipidmembranesimplicationsfortrpa1mediatedchemosensation AT yeranddyaalpizar differentialinteractionsofbacteriallipopolysaccharideswithlipidmembranesimplicationsfortrpa1mediatedchemosensation
_version_	1718388296453718016

Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation

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