Sexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish

Abstract Neurons expressing the neuropeptide hypocretin/orexin (Hcrt) in the hypothalamus promote reward-related behaviors including alcohol consumption and are shown in rodents and zebrafish to be stimulated by embryonic exposure to ethanol (EtOH). We used here in zebrafish three-dimensional analys...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Adam D. Collier, Nushrat Yasmin, Nailya Khalizova, Samantha Campbell, Amanda Onoichenco, Milisia Fam, Avi S. Albeg, Sarah F. Leibowitz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/01f9f23e379144fc8ee7fa1f28438a80
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:01f9f23e379144fc8ee7fa1f28438a80
record_format	dspace
spelling	oai:doaj.org-article:01f9f23e379144fc8ee7fa1f28438a802021-12-02T16:27:55ZSexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish10.1038/s41598-021-95707-y2045-2322https://doaj.org/article/01f9f23e379144fc8ee7fa1f28438a802021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95707-yhttps://doaj.org/toc/2045-2322Abstract Neurons expressing the neuropeptide hypocretin/orexin (Hcrt) in the hypothalamus promote reward-related behaviors including alcohol consumption and are shown in rodents and zebrafish to be stimulated by embryonic exposure to ethanol (EtOH). We used here in zebrafish three-dimensional analyses of the entire population of Hcrt neurons to examine how embryonic EtOH exposure at low-moderate concentrations (0.1% or 0.5% v/v) alters these neurons in relation to behavior. We found that EtOH in the water for 2 h (22–24 h post fertilization) increases the number of Hcrt neurons on the left but not right side of the brain through a stimulation of cell proliferation, this is accompanied by a decrease in locomotor activity under novel conditions but not after habituation, and these effects are evident in both larvae and adults indicating they are long lasting. Our analyses in adults revealed sexually dimorphic effects, with females consuming more EtOH-gelatin and exhibiting more freezing behavior along with an asymmetric increase in Hcrt neurons and males exhibiting increased aggression with no change in Hcrt. These findings suggest that a long lasting, asymmetric increase in Hcrt neurons induced by EtOH results from an asymmetric increase in proliferation specific to Hcrt and contributes to behavioral changes in females.Adam D. CollierNushrat YasminNailya KhalizovaSamantha CampbellAmanda OnoichencoMilisia FamAvi S. AlbegSarah F. LeibowitzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adam D. Collier Nushrat Yasmin Nailya Khalizova Samantha Campbell Amanda Onoichenco Milisia Fam Avi S. Albeg Sarah F. Leibowitz Sexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish
description	Abstract Neurons expressing the neuropeptide hypocretin/orexin (Hcrt) in the hypothalamus promote reward-related behaviors including alcohol consumption and are shown in rodents and zebrafish to be stimulated by embryonic exposure to ethanol (EtOH). We used here in zebrafish three-dimensional analyses of the entire population of Hcrt neurons to examine how embryonic EtOH exposure at low-moderate concentrations (0.1% or 0.5% v/v) alters these neurons in relation to behavior. We found that EtOH in the water for 2 h (22–24 h post fertilization) increases the number of Hcrt neurons on the left but not right side of the brain through a stimulation of cell proliferation, this is accompanied by a decrease in locomotor activity under novel conditions but not after habituation, and these effects are evident in both larvae and adults indicating they are long lasting. Our analyses in adults revealed sexually dimorphic effects, with females consuming more EtOH-gelatin and exhibiting more freezing behavior along with an asymmetric increase in Hcrt neurons and males exhibiting increased aggression with no change in Hcrt. These findings suggest that a long lasting, asymmetric increase in Hcrt neurons induced by EtOH results from an asymmetric increase in proliferation specific to Hcrt and contributes to behavioral changes in females.
format	article
author	Adam D. Collier Nushrat Yasmin Nailya Khalizova Samantha Campbell Amanda Onoichenco Milisia Fam Avi S. Albeg Sarah F. Leibowitz
author_facet	Adam D. Collier Nushrat Yasmin Nailya Khalizova Samantha Campbell Amanda Onoichenco Milisia Fam Avi S. Albeg Sarah F. Leibowitz
author_sort	Adam D. Collier
title	Sexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish
title_short	Sexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish
title_full	Sexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish
title_fullStr	Sexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish
title_full_unstemmed	Sexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish
title_sort	sexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/01f9f23e379144fc8ee7fa1f28438a80
work_keys_str_mv	AT adamdcollier sexuallydimorphicandasymmetriceffectsofembryonicethanolexposureonhypocretinorexinneuronsasrelatedtobehavioralchangesinzebrafish AT nushratyasmin sexuallydimorphicandasymmetriceffectsofembryonicethanolexposureonhypocretinorexinneuronsasrelatedtobehavioralchangesinzebrafish AT nailyakhalizova sexuallydimorphicandasymmetriceffectsofembryonicethanolexposureonhypocretinorexinneuronsasrelatedtobehavioralchangesinzebrafish AT samanthacampbell sexuallydimorphicandasymmetriceffectsofembryonicethanolexposureonhypocretinorexinneuronsasrelatedtobehavioralchangesinzebrafish AT amandaonoichenco sexuallydimorphicandasymmetriceffectsofembryonicethanolexposureonhypocretinorexinneuronsasrelatedtobehavioralchangesinzebrafish AT milisiafam sexuallydimorphicandasymmetriceffectsofembryonicethanolexposureonhypocretinorexinneuronsasrelatedtobehavioralchangesinzebrafish AT avisalbeg sexuallydimorphicandasymmetriceffectsofembryonicethanolexposureonhypocretinorexinneuronsasrelatedtobehavioralchangesinzebrafish AT sarahfleibowitz sexuallydimorphicandasymmetriceffectsofembryonicethanolexposureonhypocretinorexinneuronsasrelatedtobehavioralchangesinzebrafish
_version_	1718383972306649088

Sexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish

Ejemplares similares