Both haemagglutinin-specific antibody and T cell responses induced by a chimpanzee adenoviral vaccine confer protection against influenza H7N9 viral challenge
Abstract Since 2013, the outbreak or sporadic infection of a new reassortant H7N9 influenza virus in China has resulted in hundreds of deaths and thousands of illnesses. An H7N9 vaccine is urgently needed, as a licensed human vaccine against H7N9 influenza is currently not available. Here, we develo...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/01fc40478339433a96e030a84ab2587f |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:01fc40478339433a96e030a84ab2587f |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:01fc40478339433a96e030a84ab2587f2021-12-02T12:32:46ZBoth haemagglutinin-specific antibody and T cell responses induced by a chimpanzee adenoviral vaccine confer protection against influenza H7N9 viral challenge10.1038/s41598-017-02019-12045-2322https://doaj.org/article/01fc40478339433a96e030a84ab2587f2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02019-1https://doaj.org/toc/2045-2322Abstract Since 2013, the outbreak or sporadic infection of a new reassortant H7N9 influenza virus in China has resulted in hundreds of deaths and thousands of illnesses. An H7N9 vaccine is urgently needed, as a licensed human vaccine against H7N9 influenza is currently not available. Here, we developed a recombinant adenovirus-based vaccine, AdC68-H7HA, by cloning the H7N9 haemagglutinin (HA) gene into the chimpanzee adenoviral vector AdC68. The efficacy of AdC68-H7HA was evaluated in mice as well as guinea pigs. For comparison, an H7N9 DNA vaccine based on HA was also generated and tested in mice and guinea pigs. The results demonstrated that both AdC68-H7HA and the DNA vaccine prime-adenovirus boost regimen induced potent immune responses in animals and completely protected mice from lethal H7N9 influenza viral challenge. A post-immunization serum transfer experiment showed that antibody responses could completely protect against lethal challenge, while a T cell depletion experiment indicated that HA-specific CD8+ T cells responses also contributed to protection. Therefore, both HA-specific humoral immunity and cellular immunity play important roles in the protection. These data suggest that the chimpanzee adenovirus expressing HA is a promising vaccine candidate for H7N9 virus or other influenza viral subtypes.Xiang WangWeihui FuSonghua YuanXi YangYufeng SongLulu LiuYudan ChiTao ChengMan XingYan ZhangChao ZhangYong YangCaihong ZhuXiaoyan ZhangSidong XiongJianqing XuDongming ZhouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Xiang Wang Weihui Fu Songhua Yuan Xi Yang Yufeng Song Lulu Liu Yudan Chi Tao Cheng Man Xing Yan Zhang Chao Zhang Yong Yang Caihong Zhu Xiaoyan Zhang Sidong Xiong Jianqing Xu Dongming Zhou Both haemagglutinin-specific antibody and T cell responses induced by a chimpanzee adenoviral vaccine confer protection against influenza H7N9 viral challenge |
| description |
Abstract Since 2013, the outbreak or sporadic infection of a new reassortant H7N9 influenza virus in China has resulted in hundreds of deaths and thousands of illnesses. An H7N9 vaccine is urgently needed, as a licensed human vaccine against H7N9 influenza is currently not available. Here, we developed a recombinant adenovirus-based vaccine, AdC68-H7HA, by cloning the H7N9 haemagglutinin (HA) gene into the chimpanzee adenoviral vector AdC68. The efficacy of AdC68-H7HA was evaluated in mice as well as guinea pigs. For comparison, an H7N9 DNA vaccine based on HA was also generated and tested in mice and guinea pigs. The results demonstrated that both AdC68-H7HA and the DNA vaccine prime-adenovirus boost regimen induced potent immune responses in animals and completely protected mice from lethal H7N9 influenza viral challenge. A post-immunization serum transfer experiment showed that antibody responses could completely protect against lethal challenge, while a T cell depletion experiment indicated that HA-specific CD8+ T cells responses also contributed to protection. Therefore, both HA-specific humoral immunity and cellular immunity play important roles in the protection. These data suggest that the chimpanzee adenovirus expressing HA is a promising vaccine candidate for H7N9 virus or other influenza viral subtypes. |
| format |
article |
| author |
Xiang Wang Weihui Fu Songhua Yuan Xi Yang Yufeng Song Lulu Liu Yudan Chi Tao Cheng Man Xing Yan Zhang Chao Zhang Yong Yang Caihong Zhu Xiaoyan Zhang Sidong Xiong Jianqing Xu Dongming Zhou |
| author_facet |
Xiang Wang Weihui Fu Songhua Yuan Xi Yang Yufeng Song Lulu Liu Yudan Chi Tao Cheng Man Xing Yan Zhang Chao Zhang Yong Yang Caihong Zhu Xiaoyan Zhang Sidong Xiong Jianqing Xu Dongming Zhou |
| author_sort |
Xiang Wang |
| title |
Both haemagglutinin-specific antibody and T cell responses induced by a chimpanzee adenoviral vaccine confer protection against influenza H7N9 viral challenge |
| title_short |
Both haemagglutinin-specific antibody and T cell responses induced by a chimpanzee adenoviral vaccine confer protection against influenza H7N9 viral challenge |
| title_full |
Both haemagglutinin-specific antibody and T cell responses induced by a chimpanzee adenoviral vaccine confer protection against influenza H7N9 viral challenge |
| title_fullStr |
Both haemagglutinin-specific antibody and T cell responses induced by a chimpanzee adenoviral vaccine confer protection against influenza H7N9 viral challenge |
| title_full_unstemmed |
Both haemagglutinin-specific antibody and T cell responses induced by a chimpanzee adenoviral vaccine confer protection against influenza H7N9 viral challenge |
| title_sort |
both haemagglutinin-specific antibody and t cell responses induced by a chimpanzee adenoviral vaccine confer protection against influenza h7n9 viral challenge |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/01fc40478339433a96e030a84ab2587f |
| work_keys_str_mv |
AT xiangwang bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT weihuifu bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT songhuayuan bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT xiyang bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT yufengsong bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT lululiu bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT yudanchi bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT taocheng bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT manxing bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT yanzhang bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT chaozhang bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT yongyang bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT caihongzhu bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT xiaoyanzhang bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT sidongxiong bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT jianqingxu bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge AT dongmingzhou bothhaemagglutininspecificantibodyandtcellresponsesinducedbyachimpanzeeadenoviralvaccineconferprotectionagainstinfluenzah7n9viralchallenge |
| _version_ |
1718393957402017792 |